Early osteoblastic differentiation induced by dexamethasone enhances adenoviral gene delivery to marrow stromal cells

Journal of Orthopaedic Research

Volumn 22, Issue 2, 2004, Pages 411-416

  Blum, Jeremy S ^a   Parrott, M Brandon ^b   Mikos, Antonios G ^a   Barry, Michael A ^a,b,c,d  

^a Rice University   (United States)

^b BAYLOR COLLEGE OF MEDICINE   (United States)

^c CENTER FOR CELL AND GENE THERAPY   (United States)

^d NONE   (United States)

Author keywords

Dexamethasone; Gene therapy; hBMP 2; Marrow stromal cells; Osteodifferentiation

Indexed keywords

ADENOVIRUS VECTOR; ASCORBIC ACID 2 PHOSPHATE; BONE MORPHOGENETIC PROTEIN 2; DEXAMETHASONE; GLYCEROL 2 PHOSPHATE; LIPOSOME; RETROVIRUS VECTOR;

ANIMAL CELL; ARTICLE; BONE MARROW CELL; CELL CULTURE; CELL DIFFERENTIATION; CONCENTRATION RESPONSE; CONTROLLED STUDY; CULTURE MEDIUM; GENE DELIVERY SYSTEM; GENE EXPRESSION; GENETIC MANIPULATION; GENETIC TRANSDUCTION; INCUBATION TIME; MULTIPOTENT STEM CELL; NONHUMAN; OSTEOBLAST; PRIORITY JOURNAL; PROTEIN FUNCTION; RAT; STROMA CELL; TRANSGENE; VIRAL GENE THERAPY;

ADENOVIRIDAE; ALKALINE PHOSPHATASE; ANIMALS; ASCORBIC ACID; BONE MARROW CELLS; BONE MORPHOGENETIC PROTEINS; CELL DIFFERENTIATION; DEXAMETHASONE; DOSE-RESPONSE RELATIONSHIP, DRUG; DRUG COMBINATIONS; GENE EXPRESSION; GENE TRANSFER TECHNIQUES; GENETIC VECTORS; GLYCEROPHOSPHATES; LUCIFERASES; OSTEOBLASTS; RATS; RATS, WISTAR; STROMAL CELLS; TRANSFORMING GROWTH FACTOR BETA;

EID: 0742319024     PISSN: 07360266     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.orthres.2003.08.006     Document Type: Article

References (26)

1. Blum J.S. Li R.H. Mikos A.G. Barry M.A. An optimized method for the chemiluminescent detection of alkaline phosphatase levels during osteodifferentiation by bone morphogenetic protein 2 J. Cell. Biochem. 80 2001 532-537
2. Bruder S.P. Jaiswal N. Haynesworth S.E. Growth kinetics, self-renewal, and the osteogenic potential of purified human mesenchymal stem cells during extensive subcultivation and following cryopreservation J. Cell. Biochem. 64 1997 278-294
3. Bruder S.P. Kraus K.H. Goldberg V.M. Kadiyala S. The effect of implants loaded with autologous mesenchymal stem cells on the healing of canine segmental bone defects J. Bone Joint Surg. Am. 80 1998 985-996
4. Bruder S.P. Kurth A.A. Shea M. Hayes W.C. Jaiswal N. Kadiyala S. Bone regeneration by implantation of purified, culture-expanded human mesenchymal stem cells J. Orthop. Res. 16 1998 155-162
5. Cheng S.L. Lai C.F. Fausto A. Chellaiah M. Feng X. McHugh K.P. et al. Regulation of alphaVbeta3 and alphaVbeta5 integrins by dexamethasone in normal human osteoblastic cells J. Cell. Biochem. 77 2000 265-276
6. Cheng S.L. Zhang S.F. Avioli L.V. Expression of bone matrix proteins during dexamethasone-induced mineralization of human bone marrow stromal cells J. Cell. Biochem. 61 1996 182-193
7. Goshima J. Goldberg V.M. Caplan A.I. The osteogenic potential of culture-expanded rat marrow mesenchymal cells assayed in vivo in calcium phosphate ceramic blocks Clin. Orthop. 1991 298-311
8. Gysin R. Wergedal J.E. Sheng M.H. Kasukawa Y. Miyakoshi N. Chen S.T. et al. Ex vivo gene therapy with stromal cells transduced with a retroviral vector containing the BMP4gene completely heals critical size calvarial defect in rats Gene. Ther. 9 2002 991-999
9. Jaiswal N. Haynesworth S.E. Caplan A.I. Bruder S.P. Osteogenic differentiation of purified, culture-expanded human mesenchymal stem cells in vitro J. Cell. Biochem. 64 1997 295-312
10. Kadiyala S. Young R.G. Thiede M.A. Bruder S.P. Culture expanded canine mesenchymal stem cells possess osteochondrogenic potential in vivo and in vitro Cell Transplant. 6 1997 125-134
11. Kirker-Head C.A. Potential applications and delivery strategies for bone morphogenetic proteins Adv. Drug Deliv. Rev. 43 2000 65-92
12. Kon E. Muraglia A. Corsi A. Bianco P. Marcacci M. Martin I. et al. Autologous bone marrow stromal cells loaded onto porous hydroxyapatite ceramic accelerate bone repair in critical-size defects of sheep long bones J. Biomed. Mater. Res. 49 2000 328-337
13. Krebsbach P.H. Gu K. Franceschi R.T. Rutherford R.B. Gene therapy-directed osteogenesis: BMP-7-transduced human fibroblasts form bone in vivo Hum. Gene Ther. 11 2000 1201-1210
14. Krebsbach P.H. Mankani M.H. Satomura K. Kuznetsov S.A. Robey P.G. Repair of craniotomy defects using bone marrow stromal cells Transplantation 66 1998 1272-1278
15. Lieberman J.R. Daluiski A. Stevenson S. Wu L. McAllister P. Lee Y.P. et al. The effect of regional gene therapy with bone morphogenetic protein-2-producing bone-marrow cells on the repair of segmental femoral defects in rats J. Bone Joint Surg. Am. 81 1999 905-917
16. Malaval L. Modrowski D. Gupta A.K. Aubin J.E. Cellular expression of bone-related proteins during in vitro osteogenesis in rat bone marrow stromal cell cultures J. Cell. Physiol. 158 1994 555-572
17. Maniatopoulos C. Sodek J. Melcher A.H. Bone formation in vitro by stromal cells obtained from bone marrow of young adult rats Cell Tissue Res. 254 1988 317-330
18. Mathias P. Wickham T. Moore M. Nemerow G. Multiple adenovirus serotypes use alpha v integrins for infection J. Virol. 68 1994 6811-6814
19. Ohgushi H. Okumura M. Osteogenic capacity of rat and human marrow cells in porous ceramics. Experiments in athymic (nude) mice Acta Orthop. Scand. 61 1990 431-434
20. Olmsted E.A. Blum J.S. Rill D. Yotnda P. Gugala Z. Lindsey R.W. et al. Adenovirus-mediated BMP2 expression in human bone marrow stromal cells J. Cell. Biochem. 82 2001 11-21
21. Peter S.J. Liang C.R. Kim D.J. Widmer M.S. Mikos A.G. Osteoblastic phenotype of rat marrow stromal cells cultured in the presence of dexamethasone, beta-glycerolphosphate, and l-ascorbic acid J. Cell. Biochem. 71 1998 55-62
22. Reddi A.H. Role of morphogenetic proteins in skeletal tissue engineering and regeneration Nat. Biotechnol. 16 1998 247-252
23. Schmitt J.M. Hwang K. Winn S.R. Hollinger J.O. Bone morphogenetic proteins: an update on basic biology and clinical relevance J. Orthop. Res. 17 1999 269-278
24. Thies R.S. Bauduy M. Ashton B.A. Kurtzberg L. Wozney J.M. Rosen V. Recombinant human bone morphogenetic protein-2 induces osteoblastic differentiation in W-20-17 stromal cells Endocrinology 130 1992 1318-1324
25. Volek-Smith H. Urist M.R. Recombinant human bone morphogenetic protein (rhBMP) induced heterotopic bone development in vivo and in vitro Proc. Soc. Exp. Biol. Med. 211 1996 265-272
26. Yoshikawa T. Ohgushi H. Akahane M. Tamai S. Ichijima K. Analysis of gene expression in osteogenic cultured marrow/hydroxyapatite construct implanted at ectopic sites: a comparison with the osteogenic ability of cancellous bone J. Biomed. Mater. Res. 41 1998 568-573