Development of a TaqMan quantitative PCR assay specific for Cryptosporidium parvum

FEMS Microbiology Letters

Volumn 214, Issue 1, 2002, Pages 13-17

  Fontaine, Melanie ^a   Guillot, Emmanuelle ^a  

^a Ctr Tech et de Rech d'ONDEO Serv   (France)

Author keywords

Cryptosporidium parvum; Quantitative; Real time polymerase chain reaction; TaqMan

Indexed keywords

GENOMIC DNA;

ANALYTIC METHOD; ARTICLE; CRYPTOSPORIDIUM PARVUM; DILUTION; DNA SEQUENCE; EMBRYO; ENZYMATIC ASSAY; FLUORESCENCE; GENE TARGETING; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OOCYST; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; TAQMAN TECHNIQUE;

ANIMALS; CRYPTOSPORIDIUM PARVUM; DNA, PROTOZOAN; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SPECIES SPECIFICITY; TAQ POLYMERASE; WATER;

CRYPTOSPORIDIUM; CRYPTOSPORIDIUM PARVUM;

EID: 0037183388     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(02)00839-X     Document Type: Article

References (18)

1. Current W.L., Garcia L.S. Cryptosporidiosis. Clin. Lab. Med. 11:1991;873-897.
2. EPA (1999) Method 1622. Cryptosporidium in Water by Filtration/IMS/FA. EPA-821-R-99-006, US Environmental Protection Agency-Office of Water, Washington, DC.
3. Laxer M.A., Timblin B.K., Patel R.J. DNA sequences for the specific detection of Cryptosporidium parvum by the polymerase chain reaction. Am. J. Trop. Med. Hyg. 45:1991;688-694.
4. Johnson D.W., Pieniazek N.J., Griffin D.W., Misener L., Rose J.B. Development of a PCR protocol for sensitive detection of Cryptosporidium oocysts in water samples. Appl. Environ. Microbiol. 61:1995;3849-3855.
5. Hallier-Soulier S., Guillot E. Detection of cryptosporidia and Cryptosporidium parvum oocysts in environmental water samples by immunomagnetic separation-polymerase chain reaction. J. Appl. Microbiol. 89:2000;5-10.
6. Hallier-Soulier S., Guillot E. An immunomagnetic separation polymerase chain reaction assay for rapid and ultra-sensitive detection of Cryptosporidium parvum in drinking water. FEMS Microbiol. Lett. 176:1999;285-289.
7. Heid C.A., Stevens J., Livak K.J., Williams P.M. Real time quantitative PCR. Genome Res. 6:1996;986-994.
8. Hermansson A., Lindgren P.E. Quantification of ammonia-oxidizing bacteria in arable soil by real-time PCR. Appl. Environ. Microbiol. 67:2001;972-976.
9. Gruntzig V., Nold S.C., Zhou J., Tiedje J.M. Pseudomonas stutzeri nitrite reductase gene abundance in environmental samples measured by real-time PCR. Appl. Environ. Microbiol. 67:2001;760-768.
10. Martell M., Gomez J., Esteban J.I., Sauleda S., Quer J., Cabot B., Esteban R., Guardia J. High-throughput real-time reverse transcription-PCR quantitation of hepatitis C virus RNA. J. Clin. Microbiol. 37:1999;327-332.
11. Lyons S.R., Griffen A.L., Leys E.J. Quantitative real-time PCR for Porphyromonas gingivalis and total bacteria. J. Clin. Microbiol. 38:2000;2362-2365.
12. Di Giovanni, G.D., Denhart, M., Lechevallier, M.W. and Abbaszadegan, M. (1999) Quantitative Sequence Detection (QSD) of Intact and Infectious Cryptosporidium parvum Oocysts. Ninety-ninth general meeting.
13. Moss D.M., Arrowood M.J. Quantification of Cryptosporidium parvum oocysts in mouse fecal specimens using immunomagnetic particles and two-color flow cytometry. J. Parasitol. 87:2001;406-412.
14. Arrowood M.J., Hurd M.R., Mead J.R. A new method for evaluating experimental cryptosporidial parasite loads using immunofluorescent flow cytometry. J. Parasitol. 81:1995;404-409.
15. Rochelle P.A., De Leon R., Johnson A., Stewart M.H., Wolfe R.L. Evaluation of immunomagnetic separation for recovery of infectious Cryptosporidium parvum oocysts from environmental samples. Appl. Environ. Microbiol. 65:1999;841-845.
16. Stinear T., Matusan A., Hines K., Sandery M. Detection of a single viable Cryptosporidium parvum oocyst in environmental water concentrates by reverse transcription-PCR. Appl. Environ. Microbiol. 62:1996;3385-3390.
17. Livak K.J., Flood S.J.A., Marmaro J., Giusti W., Deetz K. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic and hybridization. PCR Methods Appl. 4:1995;357-362.
18. Morgan U., Weber R., Xiao L., Sulaiman I., Thompson R.C., Ndiritu W., Lal A., Moore A., Deplazes P. Molecular characterization of Cryptosporidium isolates obtained from human immunodeficiency virus-infected individuals living in Switzerland, Kenya, and the United States. J. Clin. Microbiol. 38:2000;1180-1183.