The development of a real-time quantitative-PCR method for characterisation of a Cryptosporidium parvum in vitro culturing system and assessment of drug efficacy

Molecular and Biochemical Parasitology

Volumn 121, Issue 2, 2002, Pages 279-282

  MacDonald, Louisa M ^a   Sargent, Keith ^a   Armson, Anthony ^a   Thompson, R C Andrew ^a   Reynoldson, James A ^a  

^a MURDOCH UNIVERSITY   (Australia)

Author keywords

Cryptosporidium parvum; Efficacy; In vitro culturing; Quantitative PCR

Indexed keywords

6 CARBOXYFLUORESCEIN; DYE; PROTOZOAL DNA; TRIFLURALIN;

ANALYTIC METHOD; ARTICLE; CANCER CELL CULTURE; CRYPTOSPORIDIUM PARVUM; DNA DETERMINATION; DNA EXTRACTION; DNA PROBE; DRUG EFFECT; DRUG EFFICACY; GENE FREQUENCY; GENE IDENTIFICATION; HUMAN; NONHUMAN; OOCYST; PARASITE CULTIVATION; PARASITOSIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; VALIDATION PROCESS;

ANIMALS; COCCIDIOSTATS; CRYPTOSPORIDIUM PARVUM; DNA, PROTOZOAN; HUMANS; PARASITIC SENSITIVITY TESTS; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TUMOR CELLS, CULTURED;

CRYPTOSPORIDIUM; CRYPTOSPORIDIUM PARVUM;

EID: 0036270034     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-6851(02)00041-5     Document Type: Article

References (20)

1. Development of a microtitre ELISA to quantify development of Cryptosporidium parvum in vitro
2. Method detection limits of PCR and immunofluorescence assay for Cryptosporidium parvum in soil
3. A chemiluminescence immunoassay for evaluation of Cryptosporidium parvum growth in vitro
4. Development and application of an improved semiquantitative technique for detecting low-level Cryptosporidium parvum infections in mouse tissue using polymerase chain reaction
5. A comparison of the effects of two dinitroanilines against Cryptosporidium parvum in vitro and in vivo in neonatal mice and rats
6. Simplified methods for obtaining purified oocysts from mice and for growing Cryptosporidium parvum in vitro
7. Cryptosporidiosis: An emerging, highly infectious threat
8. Efficacy of 101 antimicrobials and other agents on the development of Cryptosporidium parvum
9. In vitro anticryptosporidial activity of dinitroaniline herbicides
10. Synthesis and evaluation of dinitroanilines for treatment of cryptosporidiosis
11. Improved efficacy of dinitroaniline analogs for use as anti-cryptosporidial drugs
12. Polymerase chain reaction (PCR) as a laboratory tool for the evaluation of the parasitological cure in Chagas disease after specific treatment
13. Comparison of the ABI 7700 system (TaqMan) and competitive PCR for quantification of IS6110 DNA in sputum during treatment of tuberculosis
14. Zwischenmolekulare energie-wanderung and fluoreszenz
15. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridisation
16. Detection of specific polymerase chain reaction product by utilising the 5′ to 3′ exonuclease activity of Thermus aquaticus DNA polymerase
17. Real time quantitative PCR
18. Molecular characterisation of Cryptosporidium isolates from humans and other animals using random amplified polymorphic DNA analysis
19. The development of diagnostic PCR primers for Cryptosporidium using RAPD-PCR
20. Allelic discrimination using fluorogenic probes and the 5′ nuclease assay