Modified colorimetric assay for uricase activity and a screen for mutant Bacillus subtilis uricase genes following StEP mutagenesis

European Journal of Biochemistry

Volumn 271, Issue 3, 2004, Pages 517-523

  Huang, Su Hua ^a   Wu, Tung Kung ^a  

^a NATIONAL CHIAO TUNG UNIVERSITY   (Taiwan)

Author keywords

Bacillus subtilis uricase gene; Horseradish peroxidase; Maltose binding protein; Modified colorimetric assay; Staggered extension process (StEP)

Indexed keywords

3,5 DICHLORO 2 HYDROXYBENZENE SULFONATE; 4 AMINOPHENAZONE; BENZENE DERIVATIVE; HORSERADISH PEROXIDASE; UNCLASSIFIED DRUG; URATE OXIDASE; URIC ACID;

ARTICLE; BACILLUS SUBTILIS; COLORIMETRY; CONTROLLED STUDY; ENZYME ACTIVITY; ESCHERICHIA COLI; EUKARYOTE; MICROTITER PLATE ASSAY; MOLECULAR CLONING; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROKARYOTE; THERMOPHILIC BACTERIUM;

AMINO ACID SEQUENCE; BACILLUS SUBTILIS; BASE SEQUENCE; COLORIMETRY; DNA PRIMERS; MOLECULAR SEQUENCE DATA; MUTAGENESIS; SEQUENCE HOMOLOGY, AMINO ACID; URATE OXIDASE;

ARMORACIA RUSTICANA; BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; EUKARYOTA; POSIBACTERIA; PROKARYOTA;

EID: 0442310668     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1033.2003.03951.x     Document Type: Article

References (24)

1. Keilin, J. (1959) The biological significance of uric acid and guanine excretion. Biol. Rev. 34, 265-296.
2. Wallrath, L.L. & Friedman, T.B. (1991) Species differences in the temporal pattern of Drosophila urate oxidase gene expression are attributed to trans-acting regulatory changes. Proc. Natl Acad. Sci. USA 88, 5489-5493.
3. Montalbini, P., Redondo, J., Caballero, J.L., Cárdenas, J. & Pineda, M. (1997) Uricase from leaves: its purification and characterization from three different higher plants. Planta 202, 277-283.
4. Montalbini, P., Aguilar, M. & Pineda, M. (1999) Isolation and characterization of uricase from bean leaves and its comparison with uredospore enzyme. Plant Sci. 147, 139-147.
5. Yuichi, H., Tetsuhiko, S. & Hajime, I. (2000) Cloning, sequence analysis and expression in Escherichia coli of the gene encoding a uricase from the yeast-like symbiont of the brown planthopper, Nilaparvata lugens. Insect Biochem. Mol Biol. 30, 173-182.
6. Koyama, Y., Ichikawa, T. & Nakano, E. (1996) Cloning, sequence analysis and expression in Escherichia coli of the gene encoding the Candida utilis urate oxidase. J. Biochem. 120, 969-973.
7. Adamek, V., Suchova, M., Demnerova, K., Kralova, B., Fort, I. & Morava, P. (1990) Fermentation of Candida utilis for uricase production. J. Indu. Microbiol. 6, 85-90.
8. Yamamoto, K., Kojima, Y., Kikuchi, T., Shigyo, T., Sugihara, K. & Takashio, M. (1996) Nucleotide sequence of the uricase gene from Bacillus sp. TB-90. J. Biochem. 119, 80-84.
9. Eswara, U.S., Dutt, H. & Mottola, A. (1974) Determination of uric acid at the microgram level by a kinetic procedure based on a pseudo-induction period. Anal. Chem. 46, 1777.
10. Burtic, C.A. & Ashwood, E.R. (1994) Teitz Textbook of Clinical Chemistry, 2nd edn. WB Saunders Co. Philadelphia.
11. Yutaka, A., Hiroshi, I., Hiroomi, N., Tsugutoshi, A. & Mitsutatk, Y. (1992) Effects of serum bilirubin on determination of uric acid by the uricase-peroxidase coupled reaction. Clin. Chem. 38, 1350-1352.
12. Bhargava, A.K., Lal, H. & Pundir, C.S. (1999) Discrete analysis of serum uric acid with immobilized uricase and peroxidase. J. Biochem. Biophys. Methods 39, 125-136.
13. Nanjo, M. & Guilbault, G.G. (1974) Enzyme electrode sensing oxygen for uric acid in serum and urine. Anal. Chem. 46, 1769-1772.
14. Uchiyama, S., Shimizu, H. & Hasebe, Y. (1991) Chemical amplification of uric acid sensor responses by dithiothreitol. Anal. Chem. 66, 1873-1876.
15. Miland, E., Ordieres, A.J.M., Blanco, P.T. & Smyth, C.Ó. (1996) Poly (o-aminophenol)-modified bienzyme carbon paste electrode for the detection of uric acid. Talanta 43, 785-796.
16. Schiavon, O., Calicati, P., Ferruti, P. & Veronese, F.M. (2000) Therapeutic proteins: a comparison of chemical and biological properties of uricase conjugated to linear or branched poly (ethylene glycol) and poly (N-acryloylmorpholine). Il Farmaco 55, 264-269.
17. Huimin, Z., Lori, G., Zhixin, S., Joseph, A.A. & Frances, H.A. (1998) Molecular evolution by staggered extension process (StEP) mutagenesis in vitro recombination. Nat. Biotechnol. 16, 258-261.
18. Zhao, H. & Amold, F.H. (1997) Functional and nonfunctional mutations distinguished by random recombination of homologous genes. Proc. Natl Acad. Sci. USA 94, 7997-8000.
19. Guan, C., Li, P., Riggs, P.D. & Lnouye, H. (1988) Vectors that facilitate the expression and purification of foreign peptides in E. coli by fusion to maltose-binding protein. Gene 67, 21-30.
20. Maina, C.V., Riggs, P.D., Grandea, A.G., Slatko, B.E., Moran, L.S. & Tagliamonte, J.A. (1988) An E. coli vector to express and purify foreign proteins by fusion to and separation from maltose-binding protein. Gene 74, 365-373.
21. Kellerman, O.K. & Ferenci, Y. (1982) Maltose-binding protein from Escherichia coli. Methods Enzymol. 90, 459-467.
22. Bairoch, A. (1991) Prosite: a dictionary of sites and patterns in proteins. Nucleic Acids Res. 19, 2241-2246.
23. Legoux, R., Delpech, B., Dumont, X., Guillemot, J.C., Ramond, P., Shire, D., Caput, D., Ferrara, P. & Loison, G. (1992) Cloning and expression in Escherichia coli of the gene encoding Aspergillus flavus urate oxidase. J. Biol. Chem. 267, 8565-8570.
24. Huang, S.H., Shih, Y.C., Wu, C.Y. & Wu, T.K. (2003) Detection of serum uric acid using optical polymeric biochip detection system. Biosens. Bioelectron. in press.