Transgenic RNAi Reveals Essential Function for CTCF in H19 Gene Imprinting

Science

Volumn 303, Issue 5655, 2004, Pages 238-240

  Fedoriw, Andrew M ^a   Stein, Paula ^a   Svoboda, Petr ^a   Schultz, Richard M ^a   Bartolomei, Marisa S ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING ENERGY; GENETIC ENGINEERING; GROWTH KINETICS; PROTEINS; RNA;

GENE IMPRINTING; METHYLATION;

BIOMEDICAL ENGINEERING;

BINDING PROTEIN; PROTEIN CTCF; SOMATOMEDIN B; UNCLASSIFIED DRUG;

GENE; PROTEIN; RNA;

ARTICLE; GENE; GENE EXPRESSION; GENE FUNCTION; GENOME IMPRINTING; H19 GENE; METHYLATION; OOCYTE DEVELOPMENT; PREIMPLANTATION EMBRYO; PRIORITY JOURNAL; PROTEIN DOMAIN; RNA INTERFERENCE; TRANSGENE;

ACTINS; ANIMALS; DNA METHYLATION; DNA-BINDING PROTEINS; EMBRYONIC AND FETAL DEVELOPMENT; FEMALE; FERTILIZATION IN VITRO; GENE TARGETING; GENOMIC IMPRINTING; LITTER SIZE; MICE; MICE, TRANSGENIC; NUCLEAR PROTEINS; OOCYTES; REPRESSOR PROTEINS; RNA INTERFERENCE; RNA, MESSENGER; RNA, UNTRANSLATED; ZYGOTE;

VERTEBRATA;

EID: 0347986844     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1090934     Document Type: Article

References (27)

1. A. C. Ferguson-Smith, M. A. Surani, Science 293, 1086 (2001).
2. S. Zemel, M. S. Bartolomei, S. M. Tilghman, Nature Genet. 2, 61 (1992).
3. K. D. Tremblay, K. L. Duran, H. S. Bartolomei, Mol. Cell. Biol. 17, 4322 (1997).
4. J. L. Thorvaldsen, K. L. Duran, M. S. Bartolomei, Genes Dev. 12, 3693 (1998).
5. S. A. C. Bell, G. Felsenfeld, Nature 405, 482 (2000).
6. A. T. Hark et al., Nature 405, 486 (2000).
7. C. Kanduri et al., Curr. Biol. 10, 853 (2000).
8. C. R. Kaffer et al., Genes Dev. 14, 1908 (2000).
9. P. E. Szabo, S.-H. Tang, A. Rentsendorj, G. P. Pfeifer, J. R. Mann, Curr. Biol. 10, 607 (2000).
10. C. J. Schoenherr, J. M. Levorse, S. M. Tilghman, Nature Genet. 33, 66 (2003).
11. V. Pant et al., Genes Dev. 17, S86 (2003).
12. W. Reik, J. Walter, Nature Genet. 27, 255 (2001).
13. J. L. Thorvaldsen, H. S. Bartolomei, Science 288, 2145 (2000).
14. P. Svoboda, P. Stein, H. Hayashi, R. M. Schultz, Development 127, 4147 (2000).
15. P. Stein, P. Svoboda, R. M. Schultz. Dev. Biol. 256, 187 (2003).
16. Materials and methods are available as supporting material on Science Online.
17. A. M. Fedoriw, M. S. Bartolomei, data not shown.
18. A. Olek, J. Walter, Nature Genet. 17, 275 (1997).
19. P. M. Warnecke, S. J. Clark, Mol. Cell. Biol. 19, 164 (1999).
20. D. Lucifero, C. Mertineit, H. J. Clarke, T. H. Bestor, J. M. Trasler, Genomics 79, 530 (2002).
21. B. K. Jones, J. M. Levorse, S. M. Tilghman, Genes Dev. 12, 2200 (1998).
22. C. Y. Howell et al., Cell 104, 829 (2001).
23. D. Bourc'his, G.-L. Xu, C.-S. Lin, B. Bollman, T. H. Bestor, Science 294, 2536 (2001).
24. M. Brandeis et al., Nature 371, 435 (1994).
25. D. Macleod, J. Charlton, J. Mullins, A. P. Bird, Genes Dev. 8, 2282 (1994).
26. R. Ohlsson, R. Renkawitz, V. Lobanenkov, Trends Genet. 17, 520 (2001).
27. We thank C. Schoenherr and D. Lucifero for the bisulfite mutagenesis protocols and B. Bhogal for technical assistance. Supported by NIH (HD-42026 to M.S.B. and R.M.S. and T32 HD-07516 to A.M.F.) and the Howard Hughes Medical Institute (M.S.B.).