Investigation of the Genotoxic Potential of the Waters of a River Receiving Tannery Effluents by Means of the in vitro Comet Assay

Cytologia

Volumn 68, Issue 4, 2003, Pages 395-401

  Matsumoto, Silvia Tamie ^a   Mantovani, Mário Sérgio ^b   Mallaguti, Mirtis I ^c   Marin Morales, Maria Aparecida ^c  

^a FEDERAL UNIVERSITY OF ESPÍRITO SANTO   (Brazil)

^b STATE UNIVERSITY OF LONDRINA   (Brazil)

^c SÃO PAULO STATE UNIVERSITY UNESP   (Brazil)

Author keywords

CHO K1; Chromium; Comet assay; Genotoxicity

Indexed keywords

EID: 0347031274     PISSN: 00114545     EISSN: None     Source Type: Journal    
DOI: 10.1508/cytologia.68.395     Document Type: Article

References (14)

1. CETESB-Companhia de Tecnologia de Saneamento Ambiental. 1995. Controle de Poluição Ambiental. Legislação Estad-ual. São Paulo-Brasil. p. 98.
2. Darroudi, F. and Natarajan, A. T. 1993. Metabolic activation of chemicals to mutagenic carcinogens by human hepatoma microsomal extracts in Chinese hamster ovary cells (in vitro). Mutagenesis 8: 11-15.
3. th Ed. Washington, p. 103.
4. Klaude, M., Eriksson, S., Nygren, J. and Ahnström, G. 1996. The Comet assay: mechanisms and technical considerations. Mutat. Res. 363: 89-96.
5. Kobayashi, H., Sugiyama, C., Morikawa, Y., Hayashi, M. and Sofuni, T. 1995. A comparison between manual microscopic analysis and computerized image analysis in the single cell gel electrophoresis. MMS Commun. 3: 103-115.
6. Levis, A. G., Blanchi, V., Tamino, G. and Pegoraro, B. 1978. Citotoxic effects of hexavalent and trivalent chromium on mammalian cells in vitro. Br. J. Cancer 37: 389-396.
7. McNamee, J. P., McLean, J. R. N., Ferrarotto, C. L. and Bellier, P. V. 2000. Comet assay: rapid processing of multiple samples. Mutat. Res. 466: 63-69.
8. Mitchelmore, C. L. and Chipman, J. K. 1998. DNA strand breakage in aquatic organisms and the potential value of the comet assay in environmental monitoring. Mutat. Res. 399: 135-147.
9. Nacci, D., Nelson, S., Nelson, W. and Jackim, E. 1992. Application of the DNA alkaline unwinding assay to detect DNA strand breaks in marine bivalves. Mar. Environ. Res. 33: 83-100.
10. Olive, P. L. and Banáth, J. P. 1993. Induction and rejoining of radiation-induced DNA single-strand breaks: 'tail moment' as a function of position in the cell cycle. Mutat. Res. 294: 275-283.
11. Pandrangi, R., Petras, M., Ralph, S. and Vrzoc, M. 1995. Alkaline single cell gel (Comet) assay and genotoxicity monitoring using bullheads and carp. Environ. Mol. Mutag. 26: 345-356.
12. Pereira, A. A. B. 1991. Testes estatísticos para comparar proporções em problemas de citogenética. In: Rabello-Gay, M.N., Rogrigues, M. A. L. R. and Monteleone-Neto, R. (eds.). Mutagènese, Carcinogênese e Teratogênese: Métodos c Critérios de Avaliação, Ver. Brasil. De Genética. pp. 113-121.
13. Tice, R. R., Agurell, E., Anderson, D., Burlinson, B., Hartmann, A., Kobayashi, H., Miyamae, Y., Rojas, E., Ryu, J. C. and Sasaki, Y. F. 2000. Single Cell gel/comet assay: Guidelines for in vitro and in vivo Genetic Toxicology Testing. Environ. Mol. Mutag. 35: 206-221.
14. White, P. A. and Rasmussen. 1998. The genotoxic hazards of domestic wastes in surface waters. Mutat. Res. 410: 223-236.