Electrophoretic Isolation of Discrete Au Nanocrystal/DNA Conjugates

Nano Letters

Volumn 1, Issue 1, 2001, Pages 32-35

  Zanchet, Daniela ^b   Micheel, Christine M ^b   Parak, Wolfgang J ^b   Gerion, Daniele ^b   Alivisatos, A P ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b LAWRENCE BERKELEY NATIONAL LABORATORY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0002850435     PISSN: 15306984     EISSN: None     Source Type: Journal    
DOI: 10.1021/nl005508e     Document Type: Article

References (26)

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22. 18 The modified colloids were precipitated with NaCl, centrifuged, and redissolved to a final concentration of 1-10 μM.
23. Custom DNA were purchased from Integrated DNA Technologies (Coralville, IA). DNA sequences: 100b, 5′-XGCAGTAACGCTAT-GTGACCGAGAAGGATTCGCATTTGTAGTCTTGAGCCCGCAC- GAAACCTGGACACCCCTAAGCAACTCCGTA, CAGATGGGA-ACAGCA-3′; 80b: 5′-XGCAGTAACGCTATGTGACCGAGAAGG-ATTCGCATTTGTAGTCTTGAGCCCGCACGAAACCTGGACA- CCCCTAAGCAACTC-3′; 50b, 5′-XGCAGTAACGCTATGTGAC-CGAGAAGGATTCGCATTTGTAGTCTTGAGCCC-3′; 18b, 5′-XC-AGTCAGGCAGTCAGTCA-3′. X = 5′-thiol modifier.
24. Nanocrystal/DNA conjugates were prepared by adding a stoichiometric amount of 50 μM DNA (1,2, etc. equiv) to the gold colloid in the 0.5X TBE (Tris-borate EDTA buffer), vortexing for a few seconds and adding NaCl up to 50 mM, vortexing again, and incubating the solution at room temperature for 2 h before running the gel. The electrophoresis experiments were performed in 2 or 3% agarose gels at 100 V, 6.7 V/cm, 1 h. We used 0.5X TBE as running buffer. Gold colloids in this size range have a deep red color and can be easily visualized in the gels.
25. Herne, T. M.; Tarlov, M. J. J. Am. Chem. Soc. 1997, 119, 8916-8920.
26. TEM was performed on a Topcon EM002B electron microscope (200 kV) at NCEM, Lawrence National Laboratory (Berkeley, CA) and on a JEM-3010 (300 kV), LME-LNLS (Campinas, Brazil).