Focal contacts organization in osteoblastic cells under microgravity and cyclic deformation conditions

Advances in Space Research

Volumn 32, Issue 8, 2003, Pages 1561-1567

  Guignandon, Alain ^a   Akhouayri, Omar ^a   Laroche, Norbert ^a   Lafage Proust, Marie Hélène ^a   Alexandre, Christian ^a   Vico, Laurence ^a  

^a INSERM   (France)

Author keywords

[No Author keywords available]

Indexed keywords

CELLS; DEFORMATION; MICROGRAVITY PROCESSING; STRAIN;

CYCLIC DEFORMATION; OSTEOBLASTIC CELLS;

SPACE RESEARCH;

EXOBIOLOGY;

EID: 0346910534     PISSN: 02731177     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0273-1177(03)90396-6     Document Type: Article

References (19)

1. Brighton, C. T., B. J. Sennet, J. C. Farmer, J. P. Iannoti, C. A. Hansen, J. L. Williams, and J. Williamson, The inositol phosphate pathway as a mediator in the proliferative response of rat calvarial bone cells to cyclical biaxial mechanical strain, J. Orthop. Res., 10, 385-393, 1992.
2. Carvalho, R. S., J. L. Schaffer, L. C. Gerstenfeld, L. C., Osteoblasts induce osteopontin expression in response to attachment on fibronectin: demonstration of a common role for integrin receptors in the signal transduction processes of cell attachment and mechanical stimulation, J. Cell Biochem., 70, 376-390, 1998.
3. Carvalho, R., J. E. Scott, D M. Suga, and E. K. Yen, Stimulation of signal transduction pathways in osteoblasts by mechanical strain potentiated by parathyroid hormone, J. Bone Miner. Res., 9, 999-1010, 1994.
4. Cary, L. A., D. C. Han, and J. L. Guan, Integrin-mediated signal transduction pathways, Histol. Histopathol., 14, 1001-1009, 1999.
5. Guignandon, A., C. Genty, L. Vico, M. H. Lafage-Proust, S. Palle, and C. Alexandre, Demonstration of feasibility of automated osteoblastic line culture in space flight, Bone, 20, 109-116, 1997.
6. Guignandon, A., M. H. Lafage-Proust, Y. Usson, N. Laroche, A. Caillot-Augusseau, C. Alexandre, and L. Vico, Cell cycling determines integrin-mediated adhesion in osteoblastic ROS 17/2.8 cells exposed to space-related conditions, FASEB J., 15, 2036-2038, 2001.
7. Lai, C. F., L. Chaudhary, A. Fausto, L. R. Halstead, D. S. Ory, L. V. Avioli, and S. L. Cheng, Erk is essential for growth, differentiation, integrin expression, and cell function in human osteoblastic cells, J. Biol Chem., 276, 14443-14450, 2001.
8. Lewis, M. L., J.L. Reynolds, L. A. Cubano, J. P. Hatton, B. D. Lawless, and E. H. Piepmeier, Spaceflight alters microtubules and increases apoptosis in human lymphocytes (Jurkat), FASEB J., 12, 1007-1018, 1998.
9. Mainly, B., In: Multivariate Statistical Analysis. A primer, Chapman and Hall Ed., London and New-York, pp.86-99, 1986.
10. Miyamoto, S., H. Teramoto, O. Coso, J. Gutking, P. Burbelo, S. Akiyama, and K. Yamada, Integrin function: molecular hierarchies of cytoskeletal and signaling molecules, J. Cell Biol., 131, 791-805, 1995.
11. Nomura, S., and T. Takano-Yamamoto, Molecular events caused by mechanical stress in bone, Matrix Biol., 19, 91-96, 2000.
12. Oxlund, H., N. B. Andersen, G. Ortoft, H. Orskov, T. T. Andreassen, Growth hormone and mild exercise in combination markedly enhance cortical bone formation and strength in old rats, Endocrinology, 139, 1899-1904, 1998.
13. Rottner, K., A. Hall and J. V. Small, Interplay between Rac and Rho in the control of substrate contact dynamics, Curr. Biol., 9, 640-648, 1999.
14. Rucci, N., S. Migliaccio, B. M. Zani, A. Taranta, and A. Teti, Characterization of the osteoblast-like cell phenotype under microgravity conditions in the NASA-approved rotating wall vessel bioreactor (RWV), J. Cell Biochem., 85, 167-179, 2002.
15. Sarkar, D., T. Nagaya, K. Koga, Y. Nomura, R. Gruener, and H. Seo, Culture in vector-averaged gravity under clinostat rotation results in apoptosis of osteoblastic ROS 17/2.8 cells, J. Bone Miner. Res., 15, 489-498, 2000.
16. Schwartz, M. A., Integrins, oncogenes, and anchorage independence, J. Cell Biol., 139, 575-578, 1997.
17. Shyy, J. Y., and S. Chien, Role of integrins in cellular responses to mechanical stress and adhesion, Curr. Opin. Cell Biol., 9, 707-713, 1997.
18. Usson, Y., A. Guignandon, N. Laroche, M. H. Lafage-Proust, and L. Vico, Quantitation of cell-matrix adhesion using confocal image analysis of focal contact associated proteins and interference reflection microscopy, Cytometry, 28, 298-304, 1997.
19. Vico, L., P. Collet, A. Guignandon, M. H. Lafage-Proust, T. Thomas, M. Rehaillia, and C. Alexandre, C., Effects of long-term microgravity exposure on cancellous and cortical weight-bearing bones of cosmonauts, Lancet, 355, 1607-1611, 2000.