Refolding and purification of Apo2L/TRAIL produced as inclusion bodies in high-cell-density cultures of recombinant Escherichia coli

Biotechnology Letters

Volumn 25, Issue 24, 2003, Pages 2097-2101

  Shen, Ya Ling ^a   Xia, Xiao Xia ^a   Zhang, Yue ^a   Liu, Jian Wen ^a   Wei, Dong Zhi ^a   Yang, Sheng Li ^a  

^a EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

High cell density cultivation; Inclusion bodies; Purification; Refolding; Tumor necrosis factor related apoptosis inducing ligand

Indexed keywords

APOPTOSIS REGULATORY PROTEIN; MEMBRANE PROTEIN; RECOMBINANT PROTEIN; TNFSF10 PROTEIN, HUMAN; TUMOR NECROSIS FACTOR ALPHA; TUMOR NECROSIS FACTOR RELATED APOPTOSIS INDUCING LIGAND;

AFFINITY CHROMATOGRAPHY; ARTICLE; BIOSYNTHESIS; CELL INCLUSION; CELL SURVIVAL; CULTURE TECHNIQUE; DOSE RESPONSE; DRUG EFFECT; ESCHERICHIA COLI; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; PANCREAS TUMOR; PATHOPHYSIOLOGY; PROTEIN ENGINEERING; PROTEIN FOLDING; TUMOR CELL LINE;

APOPTOSIS REGULATORY PROTEINS; CELL CULTURE TECHNIQUES; CELL LINE, TUMOR; CELL SURVIVAL; CHROMATOGRAPHY, AFFINITY; DOSE-RESPONSE RELATIONSHIP, DRUG; ESCHERICHIA COLI; HUMANS; INCLUSION BODIES; MEMBRANE GLYCOPROTEINS; PANCREATIC NEOPLASMS; PROTEIN ENGINEERING; PROTEIN FOLDING; RECOMBINANT PROTEINS; TNF-RELATED APOPTOSIS-INDUCING LIGAND; TUMOR NECROSIS FACTOR-ALPHA;

ESCHERICHIA COLI;

EID: 0346463088     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1023/B:BILE.0000007072.56577.0c     Document Type: Article

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