Geobacter sulfurreducens has two autoregulated lexA genes whose products do not bind the recA promoter: Differing responses of lexA and recA to DNA damage

Journal of Bacteriology

Volumn 185, Issue 8, 2003, Pages 2493-2502

  Jara, Mónica ^a   Núñez, Cinthia ^b,c   Campoy, Susana ^a   Fernández de Henestrosa, Antonio R ^a   Lovley, Derek R ^b   Barbé, Jordi ^a  

^a UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

^b UNIVERSITY OF MASSACHUSETTS   (United States)

^c INSTITUTO DE BIOTECNOLOGÍA   (Mexico)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; BACTERIAL PROTEIN; DNA POLYMERASE; DNA POLYMERASE IV; GENE PRODUCT; LEXA PROTEIN; PROTEIN LEXA1; PROTEIN LEXA2; PROTEIN RUVAB; PROTEIN SSB; PROTEIN UMUDC; PROTEIN UVRA; PROTEIN UVRB; RECA PROTEIN; RECN PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GENE; DINB GENE; DNA DAMAGE; ESCHERICHIA COLI; GEL MOBILITY SHIFT ASSAY; GENE EXPRESSION REGULATION; GENE FUNCTION; GENE IDENTIFICATION; GENE SEQUENCE; GENETIC CODE; GENETIC TRANSCRIPTION; GEOBACTER SULFURREDUCENS; GRAM NEGATIVE BACTERIUM; HOMEOBOX; LEX A GENE; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN DNA BINDING; RECA GENE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; CONSENSUS SEQUENCE; DNA DAMAGE; DNA POLYMERASE BETA; DNA REPAIR; DNA-BINDING PROTEINS; ELECTROPHORETIC MOBILITY SHIFT ASSAY; GENE EXPRESSION; GENES, BACTERIAL; MOLECULAR SEQUENCE DATA; PROMOTER REGIONS (GENETICS); PROTEOBACTERIA; REC A RECOMBINASES; SEQUENCE ALIGNMENT; SERINE ENDOPEPTIDASES; TRANSCRIPTION, GENETIC;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; GEOBACTER; GEOBACTER METALLIREDUCENS; GEOBACTER SULFURREDUCENS; NEGIBACTERIA; PROKARYOTA; PROTEOBACTERIA; UNCULTURED DELTA PROTEOBACTERIUM;

EID: 0345269755     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.185.8.2493-2502.2003     Document Type: Article

References (36)

1. Brawn, K., and I. Fridovich. 1981. DNA strand scission by enzymatically generated oxygen radicals. Arch. Biochem. Biophys. 206:414-419.
2. Caccavo, F. J., D. J. Lonergan, D. R. Lovley, M. Davis, J. F. Stolz, and M. J. McInerney. 1994. Geobacter sulfurreducens sp. nov., a hydrogen and acetate oxidizing dissimilatory metal-reducing microorganism. Appl. Environ. Microbiol. 60:3752-3759.
3. Campoy, S., G. Mazón, A. R. Fernández de Henestrosa, M. Llagostera, P. Brant-Monteiro, and J. Barbé. 2002. A new regulatory DNA motif of the gamma subclass Proteobacteria: identification of the LexA protein binding site of the plant pathogen Xylella fastidiosa. Microbiology 148:3583-3597.
4. Coppi, M. V., C. Leang, S. J. Sandler, and D. Lovley. 2001. Development of a genetic system for Geobacter sulfurreducens. J. Bacteriol. 67:3180-3187.
5. Courcelle, J., A. Khodursky, B. Peter, P. O. Brown, and P. C. Hanawalt. 2001. Comparative gene expression profiles following UV exposure in wildtype and SOS-deficient Escherichia coli. Genetics 158:41-64.
6. Devereux, J., P. Haeberli, and O. Smithies. 1984. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 12:387-395.
7. Fernández de Henestrosa, A. R., T. Ogi, S. Aoyogi, D. Chafin, J. J. Hayes, H. Ohmori, and R. Woodgate. 2000. Identification of additional genes belonging to the LexA regulon in Escherichia coli. Mol. Microbiol. 35:1560-1572.
8. Imlay, J. A., and S. Linn. 1988. DNA damage and oxygen radical toxicity. Science 240:1302-1309.
9. Keyer, K., and J. A. Imlay. 1996. Superoxide accelerates DNA damage by elevating free-iron levels. Proc. Natl. Acad. Sci. USA 93:13635-13640.
10. Khil, P. P., and R. D. Camerini-Otero. 2002. Over 1000 genes are involved in the DNA damage response of Escherichia coli. Mol. Microbiol. 44:89-105.
11. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
12. Lesko, S. A., R. J. Lorentzen, and P. O. Ts'o. 1980. Role of superoxide in deoxyribonucleic acid strand scission. Biochemistry 19:3023-3028.
13. Little, J. W. 1991. Mechanism of specific LexA cleavage: autodigestion and the role of RecA coprotease. Biochimie 73:411-422.
14. Little, J. W., D. Mount, and C. R. Yanish-Perron. 1981. Purified LexA protein is a repressor of the recA and lexA genes. Proc. Natl. Acad. Sci. USA 78:4199-4203.
15. Liu, Y., and H. M. Fletcher. 2001. The recA gene in Porphyromonas gingivalis is expressed during infection of the murine host. Oral Microbiol. Immunol. 16:218-233.
16. Lovley, D. R. 2000. Fe(III) and Mn(IV) reduction, p. 3-30. In D. R. Lovley (ed.), Environmental microbe-metal interactions. ASM Press, Washington, D.C.
17. Luo, Y., R. A. Pfuetzner, S. Mosimann, M. Paetzel, E. A. Frey, M. Cherney, B. Kim, J. W. Little, and C. J. Strynadka. 2001. Crystal structure of LexA: a conformational switch for regulation of self-cleavage. Cell 106:585-594.
18. McCord, J. M., and E. D. Day. 1978. Superoxide-dependent production of hydroxyl radical catalyzed by iron-EDTA complex. FEBS Lett. 86:139-142.
19. Miki, T., Y. Shirabe, Y. Ebina, and A. Nakazawa. 1984. Transcription analysis of the lexA gene of Escherichia coli: attenuation and cotranscription with the neighboring region. Nucleic Acids Res. 12:1203-1217.
20. Norioka, N., M. Y. Hsu, S. Inouye, and M. Inouye. 1995. Two recA genes in Myxococcus xanthus. J. Bacteriol. 177:4179-4182.
21. Panina, E. M., A. A. Mironov, and M. S. Gelfand. 2001. Comparative analysis of Fur regulons in γ-proteobacteria. Nucleic Acids Res. 29:5195-5206.
22. Ratledge, C., and L. G. Dover. 2000. Iron metabolism in pathogenic bacteria. Annu. Rev. Microbiol. 54:881-941.
23. Rowley, D. A., and B. Halliwell. 1982. Superoxide-dependent formation of hydroxyl radicals from NADH and NADPH in the presence of iron salts. FEBS Lett. 142:39-41.
24. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1992. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
25. Sanger, F., S. Nicklen, and S. Coulson. 1977. DNA sequencing with chainterminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
26. Sassanfar, M., and J. W. Roberts. 1990. Nature of SOS-inducing signal in Escherichia coli: the involvement of DNA replication. J. Mol. Biol. 212:79-96.
27. Serkin, C. D., and H. S. Seifert. 2000. Iron availability regulates DNA recombination in Neisseria gonorrhoeae. Mol. Microbiol. 37:1075-1086.
28. Takeuchi, T., N. Kato, K. Watanabe, and K. Morimoto. 2000. Mechanism of oxidative DNA damage induction in a strict anaerobe, Prevotella melaninogenica. FEMS Microbiol. Lett. 192:133-138.
29. Tapias, A., and J. Barbé. 1999. Regulation of divergent transcription from the uvrA-ssb promoters in Sinorhizobium meliloti. Mol. Gen. Genet. 262:121-130.
30. Tapias, A., S. Campoy, and J. Barbé. 2000. Analysis of the expression of the Rhodobacter spheroides lexA gene. Mol. Gen. Genet. 263:957-965.
31. Thompson, J. D., D. G. Higgins, and T. J. Gibson. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680.
32. Touati, D., M. Jacques, B. Tardat, L. Bouchard, and S. Despied. 1995. Lethal oxidative damage and mutagenesis are generated by iron in Δfur mutants of Escherichia coli: protective role of superoxide dismutase. J. Bacteriol. 177:2305-2314.
33. Wagner, J., P. Gruz, S. R. Kim, M. Yamada, K. Matsui, R. P. P. Fuchs, and T. Nohmi. 1999. The dinB gene encodes a novel E. coli DNA polymerase, DNA polIV, involved in mutagenesis. Mol. Cell 4:281-286.
34. Walker, G. C. 1984. Mutagenesis and inducible responses to deoxyribonucleic acid damage in Escherichia coli. Microbiol. Rev. 48:60-93.
35. Winterbourn, C. C. 1979. Comparison of superoxide with other reducing agents in the biological production of hydroxyl radicals. Biochem. J. 182:625-628.
36. Winterling, K. W., D. Chafin, J. J. Hayes, J. Sun, A. S. Levine, R. E. Yasbin, and R. Woodgate. 1998. The Bacillus subtilis DinR binding site: redefinition of the consensus sequence. J. Bacteriol. 180:2201-2211.