The Bacillus subtilis DinR binding site: Redefinition of the consensus sequence

Journal of Bacteriology

Volumn 180, Issue 8, 1998, Pages 2201-2211

  Winterling, Kevin W ^a,b   Chafin, David ^c   Hayes, Jeffery J ^c   Sun, J I ^b,d   Levine, Arthur S ^a   Yasbin, Ronald E ^d   Woodgate, Roger ^a  

^a EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT   (United States)

^b UNIVERSITY OF MARYLAND BALTIMORE COUNTY   (United States)

^c UNIVERSITY OF ROCHESTER SCHOOL OF MEDICINE AND DENTISTRY   (United States)

^d UNIVERSITY OF TEXAS AT DALLAS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA;

ARTICLE; BACILLUS SUBTILIS; BINDING SITE; CONSENSUS SEQUENCE; CONTROLLED STUDY; DNA FOOTPRINTING; ELECTROPHORETIC MOBILITY; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN LOCALIZATION; PROTEIN PURIFICATION; REPRESSOR GENE; SEQUENCE HOMOLOGY;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); BACTERIUM SUBTILIS; DINA; NEGIBACTERIA; POSIBACTERIA; UNIDENTIFIED BACTERIOPHAGE;

EID: 0031943348     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.180.8.2201-2211.1998     Document Type: Article

References (28)

1. Carey, J. 1991. Gel retardation. Methods Enzymol. 208:103-117.
2. Cheo, D. L., K. W. Bayles, and R. E. Yasbin. 1991. Cloning and characterization of DNA damage-inducible promoter regions from Bacillus subtilis. J. Bacteriol. 173:1696-1703.
3. + gene. Biochimie 74:755-762.
4. Durbach, S. I., S. J. Andersen, and V. Mizrahi. 1997. SOS induction in mycohacteria: analysis of the DNA-binding activity of a LexA-like represser and its role in DNA damage induction of the recA gene from Mycobacterium smegmatis. Mol. Microbiol. 26:643-653.
5. Haijema, B. J., D. van Sinderen, K. Winterling, J. Kooistra, G. Venema, and L. W. Hamoen. 1996. Regulated expression of the dinR and recA genes during competence development and SOS induction in Bacillus subtilis. Mol. Microbiol. 22:75-85.
6. Hayes, J. J., and T. D. Tullius. 1989. The missing nucleoside experiment: a new technique to study recognition of DNA by protein. Biochemistry 28: 9521-9527.
7. Howard-Flanders, P., and R. P. Boyce. 1966. DNA repair and genetic recombination: studies on mutants of Escherichia coli defective in these processes. Radiat. Res. 6(Suppl.):156-184.
8. Kim, B., and J. W. Little. 1992. Dimerization of a specific DNA-binding protein on the DNA. Science 255:203-206.
9. Knegtel, R. M. A., R. H. Fogh, G. Ottleben, H. Rüterjans, P. Dumoulin, M. Schnarr, R. Boelens, and R. Kaptein. 1995. A model for the LexA repressor DNA complex. Proteins 21:226-236.
10. Koch, W. H., and R. Woodgate. 1998. The SOS response, p. 107-134. In J. A. Nickoloff and M. F. Hoekstra (ed.), DNA damage and repair: DNA repair in prokaryotes and lower eukaryotes. Humana Press, Totowa, N.J.
11. Koudelka, G. B., P. Harbury, S. C. Harrison, and M. Ptashne. 1988. DNA twisting and the affinity of bacteriophage 434 operator for bacteriophage 434 repressor. Proc. Natl. Acad. Sci. USA 85:4633-4637.
12. Koudelka, G. B., S. C. Harrison, and M. Ptashne. 1987. Effect of noncontacted bases on the affinity of 434 operator for 434 repressor and Cro. Nature 326:886-888.
13. Lewis, L. K., G. R. Harlow, L. A. Gregg-Jolly, and D. W. Mount. 1994. Identification of high affinity binding sites for LexA which define new DNA damage-inducible genes in Escherichia coli. J. Mol. Biol. 241:507-523.
14. Little, J. W. 1984. Autodigestion of LexA and phage repressors. Proc. Natl. Acad. Sci. USA 81:1375-1379.
15. Miller, J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
16. Miller, M. C., J. B. Resnick, B. T. Smith, and C. M. Lovett, Jr. 1996. The Bacillus sibtilis dinR gene codes for the analogue of Escherichia coli LexA. Purification and characterization of the DinR protein. J. Biol. Chem. 271: 33502-33508.
17. Movahedzadeh, F., M. J. Colston, and E. O. Davis. 1997. Characterization of Mycobacterium tuberculosis LexA: recognition of a Cheo (Bacillus-type SOS) box. Microbiology 143:929-936.
18. Orchard, K., and G. E. May. 1993. An EMSA-hased method for determining the molecular weight of a protein-DNA complex. Nucleic Acids Res. 21: 3335-3336.
19. Papavinasasundaram, K. G., F. Movahedzadeh, J. T. Keer, N. G. Stoker, M. J. Colston, and E. O. Davis. 1997. Mycobacterial recA is cotranscribed with a potential regulatory gene called recX. Mol. Microbiol. 24:141-153.
20. Raymond-Denise, A., and N. Guillen. 1991. Identification of dinR. a DNA damage-inducible regulator gene of Bacillus subtilis. J. Bacteriol. 173:7084-7091.
21. Raymond-Denise, A., and N. Guillen. 1992. Expression of the Bacillus subtilis dinR and recA genes after DNA damage and during competence. J. Bacteriol. 174:3171-3176.
22. Schnarr, M., M. Granger-Schnarr, S. Hurstel, and J. Pouyet. 1988. The carboxy-terminal domain of the LexA repressor oligomerises essentially as the entire protein. FEBS Lett. 234:56-60.
23. Schnarr, M., J. Pouyet, M. Granger-Schnarr, and M. Daune. 1985. Largescale purification, oligomerization equilibria, and specific interaction of the LexA repressor of Escherichia coli. Biochemistry 24:2812-2818.
24. Sun, J. 1997. Ph.D. thesis. University of Maryland, Baltimore County.
25. Thliveris, A. T., J. W. Little, and D. W. Mount. 1991. Repression of the E. coli recA gene requires at least two LexA protein monomers. Biochimie 73:449-456.
26. Wertman, K. F., and D. W. Mount. 1985. Nucleotide sequence binding specificity of the LexA repressor of Escherichia coli K-12. J. Bacteriol. 163: 376-384.
27. Winterling, K. W., A. S. Levine, R. E. Yasbin, and R. Woodgate. 1997. Characterization of DinR, the Bacillus subtilis SOS repressor. J. Bacteriol. 179:1698-1703.
28. Yasbin, R. E., D. L. Cheo, and K. W. Bayles. 1992. Inducible DNA repair and differentiation in Bacillus subtilis: interactions between global regulons. Mol. Microbiol. 6:1263-1270.