Crystal structure of LexA: A conformational switch for regulation of self-cleavage

Cell

Volumn 106, Issue 5, 2001, Pages 585-594

  Luo, Yu ^a   Pfuetzner, Richard A ^a   Mosimann, Steve ^a   Paetzel, Mark ^a   Frey, Elizabeth A ^a   Cherney, Maia ^b   Kim, Baek ^c   Little, John W ^c,d   Strynadka, Natalie C J ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^b UNIVERSITY OF ALBERTA   (Canada)

^c UNIVERSITY OF ARIZONA   (United States)

^d University of Arizona   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; LEXA PROTEIN; RECA PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN CONFORMATION; PROTEIN DEGRADATION; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE; STIMULUS; STRUCTURE ANALYSIS;

BACTERIA (MICROORGANISMS);

EID: 0035823008     PISSN: 00928674     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0092-8674(01)00479-2     Document Type: Article

References (51)

1. A fast algorithm for rendering space-filling molecule pictures
2. ALSCRIPT: A tool to format multiple sequence alignments
3. Crystal structure of the lambda repressor C-terminal domain provides a model for cooperative operator binding
4. Crystallography & NMR system: A new software suite for macromolecular structure determination
5. UmuD mutagenesis protein of Escherichia coli: Overproduction, purification, and cleavage by RecA
6. Identification, classification, and analysis of beta-bulges in proteins
7. Structural and thermodynamic consequences of burying a charged residue within the hydrophobic core of T4 lysozyme
8. P22 c2 repressor. Domain structure and function
9. Dimerization of the UmuD′ protein in solution and its implications for regulation of SOS mutagenesis
10. Solution structure of the LexA repressor DNA binding domain determined by 1H NMR spectroscopy
11. Cation-pi interactions in structural biology
12. Lambda repressor inactivation: Properties of purified ind- proteins in the autodigestion and RecA-mediated cleavage reactions
13. Classical eletrostatics in biology and chemistry
14. In vitro binding of LexA repressor to DNA: Evidence for the involvement of the amino-terminal domain
15. Improved methods for binding protein models in electron density maps and the location of errors in these models
16. LexA and lambda Cl repressors as enzymes: Specific cleavage in an intermolecular reaction
17. Molscript: A program to produce both detailed and schematic plots of protein structure
18. Serine proteases: Structure and mechanism of catalysis
19. Automated refinement for protein crystallography
20. What can the structures of enzyme-inhibitor complexes tell us about the structures of enzyme substrate complexes?
21. Role of Ser-652 and Lys-692 in the protease activity of infectious bursal disease virus VP4 and identification of its substrate cleavage sites
22. Crystal structures of the photosystem II D1 C-terminal processing protease
23. Isolation and characterization of noncleavable (Ind-) mutants of the LexA repressor of Escherichia coli K-12
24. Autodigestion and RecA-dependent cleavage of Ind- mutant LexA proteins
25. Autodigestion of lexA and phage lambda repressors
26. Mechanism of specific LexA cleavage: Autodigestion and the role of RecA coprotease
27. LexA cleavage and other self-processing reactions
28. The SOS regulatory system of Escherichia coli
29. Cleavage of the Escherichia coli lexA protein by the recA protease
30. Cleavage of LexA repressor
31. Intermolecular cleavage by UmuD-like mutagenesis proteins
32. Intermolecular cleavage by UmuD-like enzymes: Identification of residues required for cleavage and substrate specificity
33. XtalView/Xfit - A versatile program for manipulating atomic coordinates and electron density
34. AMoRe: An automated package for molecular replacement
35. A model for a umuDC-dependent prokaryotic DNA damage checkpoint
36. Processing of x-ray diffraction data collected in oscillation mode
37. Crystal structure of a bacterial signal peptidase in complex with a beta-lactam inhibitor
38. Chemical and mutagenic investigations of fatty acid amide hydrolase: Evidence for a family of serine hydrolases with distinct catalytic properties
39. Structure of the UmuD′ protein and its regulation in response to DNA damage
40. The UmuD′ protein filament and its potential role in damage induced mutagenesis
41. Escherichia coli recA gene product inactivates phage lambda repressor
42. Reaction of LexA repressor with diisopropyl fluorophosphate. A test of the serine protease model
43. In vitro analysis of mutant LexA proteins with an increased rate of specific cleavage
44. Large-scale purification, oligomerization equilibria, and specific interaction of the LexA repressor of Escherichia coli
45. Mutant LexA proteins with specific defects in autodigestion
46. Lysine-156 and serine-119 are required for LexA repressor cleavage: A possible mechanism
47. The role of electrostatic interactions in the mechanism of peptide bond hydrolysis by a Ser-Lys catalytic dyad
48. ClUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice
49. Two-state allosteric behavior in a single-domain signaling protein