Molecular epidemiological analysis of Cryptosporidium isolates from humans and animals by using a heteroduplex mobility assay and nucleic acid sequencing based on a small double-stranded RNA element

Journal of Clinical Microbiology

Volumn 41, Issue 3, 2003, Pages 981-992

  Leoni, Francesca ^a,b,c   Gallimore, Chris I ^a   Green, Jonathan ^a   McLauchlin, Jim ^a  

^a CENTRAL PUBLIC HEALTH LABORATORY   (United Kingdom)

^b UNIVERSITY OF GREENWICH   (United Kingdom)

^c Ist Zooprofilattico Sperim U M   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; DOUBLE STRANDED RNA;

ARTICLE; CRYPROSPORIDIUM MELEAGRIDIS; CRYPTOSPORIDIUM; CRYPTOSPORIDIUM FELIS; CRYPTOSPORIDIUM PARVUM; EPIDEMIC; EPIDEMIOLOGY; GENOTYPE; HUMAN; LIVESTOCK; NONHUMAN; NUCLEOTIDE SEQUENCE; PARASITE ISOLATION; PRIORITY JOURNAL; SEQUENCE ANALYSIS;

ANIMAL DISEASES; ANIMALS; BASE SEQUENCE; CRYPTOSPORIDIOSIS; CRYPTOSPORIDIUM; DISEASE OUTBREAKS; EPIDEMIOLOGY, MOLECULAR; HETERODUPLEX ANALYSIS; HUMANS; MOLECULAR SEQUENCE DATA; MUTATION; RNA, DOUBLE-STRANDED; RNA, PROTOZOAN; SEQUENCE HOMOLOGY, NUCLEIC ACID;

ANIMALIA; CRYPTOSPORIDIUM; CRYPTOSPORIDIUM FELIS; CRYPTOSPORIDIUM MELEAGRIDIS; CRYPTOSPORIDIUM PARVUM; FELIS; PROTOZOA;

EID: 0345269282     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.41.3.981-992.2003     Document Type: Article

References (51)

1. Anonymous. 1999. Surveillance of waterborne disease and water quality. January to June 1999, and summary 1998. Public Health Lab. Serv. Commun. Dis. Rep. 9:305-308.
2. Anonymous. 1999. Surveillance of waterborne disease and water quality: January to June 1998. Public Health Lab. Serv. Commun. Dis. Rep. 9:73-74.
3. Anonymous. 2000. Surveillance of waterborne disease and water quality: July to December 1999. Public Health Lab. Serv. Commun. Dis. Rep. 10:65-68.
4. Arrowood, M. 1997. Diagnosis, p. 43-64. In R. Fayer (ed.), Cryptosporidium and cryptosporidiosis. CRC Press, Inc., Boca Raton, Fla.
5. Boom, R., C. J. A. Sol, M. M. M. Salimans, C. L. Jansen, P. M. E. Wertheimvan Dillen, and J. Van der Noordaa. 1990. Rapid and simple method for purification of nucleic acids. J. Clin. Microbiol. 28:495-503.
6. Bouchier, I. 1998. Cryptosporidium in water supplies: third report of the group of experts. Her Majesty's Stationery Office, London, United Kingdom.
7. Cacció, S., W. Homan, R. Camilli, G. Traldi, T. Kortbeek, and E. Pozio. 2000. A microsatellite marker reveals population heterogeneity within human and animal genotypes of Cryptosporidium parvum. Parasitology 120: 237-244.
8. Cacció, S., W. Homan, K. van Dijk, and E. Pozio. 1999. Genetic polymorphism at the beta-tubulin locus among human and animal isolates of Cryptosporidium parvum. FEMS Microbiol. Lett. 170:173-179.
9. Cacció, S., F. Spano, and E. Pozio. 2001. Large sequence variation at two microsatellite loci among zoonotic (genotype C) isolates of Cryptosporidium parvum. Int. J. Parasitol. 31:1082-1086.
10. Delwart, E. L., E. G. Shpaer, J. Louwagie, F. E. McCutchan, M. Grez, H. Rubsamen-Waigmann, and J. I. Mullins. 1993. Genetic relationships determined by a DNA heterduplex mobility assay: analysis of HIV-1 env genes. Science 262:1257-1261.
11. Fayer, R. 1997. The general biology of Cryptosporidium, p. 1-41. In R. Fayer (ed.), Cryptosporidium and cryptosporidiosis. CRC Press, Inc., Boca Raton, Fla.
12. Fayer, R., U. Morgan, and S. J. Upton. 2000. Epidemiology of Cryptosporidium: transmission, detection and identification. Int. J. Parasitol. 30:1305-1322.
13. Feng, X., S. M. Rich, D. Akiyoshi, J. K. Tumwine, A. Kekitiinwa, N. Nabukeera, S. Tzipori, and G. Widmer. 2000. Extensive polymorphism in Cryptosporidium parvum identified by multilocus microsatellite analysis. Appl. Environ. Microbiol. 66:3344-3349.
14. Gatei, W., R. W. Ashford, N. J. Beeching, S. K. Kamwati, J. Greensill, and C. A. Hart. 2002. Cryptosporidium muris infection in an HIV-infected adult, Kenya. Emerg. Infect. Dis. 8:204-206.
15. Glaberman, S., J. E. Moore, C. J. Lowery, R. M. Chalmers, I. Sulaiman, K. Elwin, P. J. Rooney, B. C. Millar, J. S. Dooley, A. A. Lal, and L. Xiao. 2002. Three drinking-water-associated cryptosporidiosis outbreaks, Northern Ireland. Emerg. Infect. Dis. 8:631-633.
16. Guyot, K., A. Follet-Dumoulin, E. Lelievre, C. Sarfati, M. Rabodonirina, G. Nevez, J. C. Cailliez, D. Camus, and E. Dei-Cas. 2001. Molecular characterization of Cryptosporidium isolates obtained from humans in France. J. Clin. Microbiol. 39:3472-3480.
17. Hall, T. A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp. Ser. 41:95-98.
18. Johnson, D. W., N. J. Pieniazek, D. W. Griffin, L. Misener, and J. B. Rose. 1995. Development of a PCR protocol for sensitive detection of Cryptosporidium oocysts in water samples. Appl. Environ. Microbiol. 61:3849-3855.
19. Khramtsov, N. V., P. A. Chung, C. C. Dykstra, J. K. Griffiths, U. M. Morgan, M. J. Arrowood, and S. J. Upton. 2000. Presence of double-stranded RNAs in human and calf isolates of Cryptosporidium parvum. J. Parasitol. 86:275-282.
20. Khramtsov, N. V., K. M. Woods, M. V. Nesterenko, C. C. Dykstra, and S. J. Upton. 1997. Virus-like, double-stranded RNAs in the parasitic protozoan Cryptosporidium parvum. Mol. Microbiol. 26:289-300.
21. Leoni, F., C. I. Gallimore, J. Green, and J. McLauchlin. A rapid method for identifying diversity within PCR amplicons using a heteroduplex mobility assay and synthetic polynucleotides: application to characterisation of dsRNA elements associated with Cryptosporidium. J. Microbiol. Methods, in press.
22. McLauchlin, J., C. Amar, S. Pedraza-Díaz, and G. L. Nichols. 2000. Molecular epidemiological analysis of Cryptosporidium spp. in the United Kingdom: results of genotyping Cryptosporidium spp. in 1,705 fecal samples from humans and 105 fecal samples from livestock animals. J. Clin. Microbiol. 38:3984-3990.
23. McLauchlin, J., D. P. Casemore, S. Moran, and S. Patel. 1998. The epidemiology of cryptosporidiosis: application of experimental sub-typing and antibody detection systems to the investigation of water-borne outbreaks. Folia Parasitol. (Prague) 45:83-92.
24. McLauchlin, J., S. Pedraza-Diaz, C. Amar-Hoetzeneder, and G. L. Nichols. 1999. Genetic characterization of Cryptosporidium strains from 218 patients with diarrhea diagnosed as having sporadic cryptosporidiosis. J. Clin. Microbiol. 37:3153-3158.
25. Meinhardt, P. L., D. P. Casemore, and K. B. Miller. 1996. Epidemiologic aspects of human cryptosporidiosis and the role of waterborne transmission. Epidemiol. Rev. 18:118-136.
26. Morgan, U., R. Weber, L. Xiao, I. Sulaiman, R. C. Thompson, W. Ndiritu, A. Lal, A. Moore, and P. Deplazes. 2000. Molecular characterization of Cryptosporidium isolates obtained from human immunodeficiency virus-infected individuals living in Switzerland, Kenya, and the United States. J. Clin. Microbiol. 38:1180-1183.
27. Morgan, U. M., C. C. Constantine, D. A. Forbes, and R. C. Thompson. 1997. Differentiation between human and animal isolates of Cryptosporidium parvum using rDNA sequencing and direct PCR analysis. J. Parasitol. 83:825-830.
28. Morgan, U. M., L. Xiao, R. Fayer, A. A. Lal, and R. C. Thompson. 1999. Variation in Cryptosporidium: towards a taxonomic revision of the genus. Int. J. Parasitol. 29:1733-1751.
29. Okhuysen, P. C., C. L. Chappell, J. H. Crabb, C. R. Sterling, and H. L. DuPont. 1999. Virulence of three distinct Cryptosporidium parvum isolates for healthy adults. J. Infect. Dis. 180:1275-1281.
30. Ong, C. S., D. L. Eisler, A. Alikhani, V. W. Fung, J. Tomblin, W. R. Bowie, and J. L. Isaac-Renton. 2002. Novel Cryptosporidium genotypes in sporadic cryptosporidiosis cases: first report of human infections with a cervine genotype. Emerg. Infect. Dis. 8:263-268.
31. Pedraza-Díaz, S., C. Amar, A. M. Iversen, P. J. Stanley, and J. McLauchlin. 2001. Unusual Cryptosporidium species recovered from human faeces: first description of Cryptosporidium felis and Cryptosporidium 'dog type' from patients in England. J. Med. Microbiol. 50:293-296.
32. Pedraza-Díaz, S., C. Amar, and J. McLauchlin. 2000. The identification and characterisation of an unusual genotype of Cryptosporidium from human faeces as Cryptosporidium meleagridis. FEMS Microbiol. Lett. 189:189-194.
33. Pedraza-Díaz, S., C. Amar, G. L. Nichols, and J. McLauchlin. 2001. Nested polymerase chain reaction for amplification of the Cryptosporidium oocyst wall protein gene. Emerg. Infect. Dis. 7:49-56.
34. Pedraza-Díaz, S., C. F. Amar, J. McLauchlin, G. L. Nichols, K. M. Cotton, P. Godwin, A. M. Iversen, L. Milne, J. R. Mulla, K. Nye, H. Panigrahl, S. R. Venn, R. Wiggins, M. Williams, and E. R. Youngs. 2001. Cryptosporidium meleagridis from humans: molecular analysis and description of affected patients. J. Infect. 42:243-250.
35. Peng, M. M., O. Matos, W. Gatei, P. Das, M. Stantic-Pavlinic, C. Bern, I. M. Sulaiman, S. Glaberman, A. A. Lal, and L. Xiao. 2001. A comparison of Cryptosporidium subgenotypes from several geographic regions. J. Eukaryot. Microbiol. (2001 Suppl.):28S-31S.
36. Peng, M. M., L. Xiao, A. R. Freeman, M. J. Arrowood, A. A. Escalante, A. C. Weltman, C. S. Ong, W. R. Mac Kenzie, A. A. Lal, and C. B. Beard. 1997. Genetic polymorphism among Cryptosporidium parvum isolates: evidence of two distinct human transmission cycles. Emerg. Infect. Dis. 3:567-573.
37. Pieniazek, N. J., F. J. Bornay-Llinares, S. B. Slemenda, A. J. da Silva, I. N. Moura, M. J. Arrowood, O. Ditrich, and D. G. Addiss. 1999. New Cryptosporidium genotypes in HIV-infected persons. Emerg. Infect. Dis. 5:444-449.
38. Spano, F., L. Putignani, A. Crisanti, P. Sallicandro, U. M. Morgan, S. M. Le Blancq, L. Tehack, S. Tzipori, and G. Widmer. 1998. Multilocus genotypic analysis of Cryptosporidium parvum isolates from different hosts and geographical origins. J. Clin. Microbiol. 36:3255-3259.
39. Spano, F., L. Putignani, S. Guida, and A. Crisanti. 1998. Cryptosporidium parvum: PCR-RFLP analysis of the TRAP-C1 (thrombospondin-related adhesive protein of Cryptosporidium-1) gene discriminates between two alleles differentially associated with parasite isolates of animal and human origin. Exp. Parasitol. 90:195-198.
40. Spano, F., L. Putignani, J. McLauchlin, D. P. Casemore, and A. Crisanti. 1997. PCR-RFLP analysis of the Cryptosporidium oocyst wall protein (COWP) gene discriminates between C. wrairi and C. parvum, and between C. parvum isolates of human and animal origin. FEMS Microbiol. Lett. 150:209-217.
41. Strong, W. B., J. Gut, and R. G. Nelson. 2000. Cloning and sequence analysis of a highly polymorphic Cryptosporidium parvum gene encoding a 60-kilodalton glycoprotein and characterization of its 15- and 45-kilodalton zoite surface antigen products. Infect. Immun. 68:4117-4134.
42. Sulaiman, I. M., A. A. Lal, and L. Xiao. 2001. A population genetic study of the Cryptosporidium parvum human genotype parasites. J. Eukaryot. Microbiol. (2001 Suppl.):24S-27S.
43. Sulaiman, I. M., U. M. Morgan, R. C. Thompson, A. A. Lal, and L. Xiao. 2000. Phylogenetic relationships of Cryptosporidium parasites based on the 70-kilodalton heat shock protein (HSP70) gene. Appl. Environ. Microbiol. 66:2385-2391.
44. Sulaiman, I. M., L. Xiao, C. Yang, L. Escalante, A. Moore, C. B. Beard, M. J. Arrowood, and A. A. Lal. 1998. Differentiating human from animal isolates of Cryptosporidium parvum. Emerg. Infect. Dis. 4:681-685.
45. Tiangtip, R., and S. Jongwutiwes. 2002. Molecular analysis of Cryptosporidium species isolated from HIV-infected patients in Thailand. Trop. Med. Int. Health 7:357-364.
46. Widmer, G., S. Tzipori, C. J. Fichtenbaum, and J. K. Griffiths. 1998. Genotypic and phenotypic characterization of Cryptosporidium parvum isolates from people with AIDS. J. Infect. Dis. 178:834-840.
47. Willocks, L., A. Crampin, L. Milne, C. Seng, M. Susman, R. Gair, M. Moulsdale, S. Shafi, R. Wall, R. Wiggins, N. Lightfoot, et al. 1998. A large outbreak of cryptosporidiosis associated with a public water supply from a deep chalk borehole. Commun. Dis. Public Health 1:239-243.
48. Xiao, L., C. Bern, M. Arrowood, I. Sulaiman, L. Zhou, V. Kawai, A. Vivar, A. A. Lal, and R. H. Gilman. 2002. Identification of the Cryptosporidium pig genotype in a human patient. J. Infect. Dis. 185:1846-1848.
49. Xiao, L., C. Bern, J. Limor, I. Sulaiman, J. Roberts, W. Checkley, L. Cabrera, R. H. Gilman, and A. A. Lal. 2001. Identification of 5 types of Cryptosporidium parasites in children in Lima, Peru. J. Infect. Dis. 183:492-497.
50. Xiao, L., J. Limor, C. Bern, and A. A. Lal. 2001. Tracking Cryptosporidium parvum by sequence analysis of small double-stranded RNA. Emerg. Infect. Dis. 7:141-145.
51. Xiao, L., U. M. Morgan, J. Limor, A. Escalante, M. Arrowood, W. Shulaw, R. C. Thompson, R. Fayer, and A. A. Lal. 1999. Genetic diversity within Cryptosporidium parvum and related Cryptosporidium species. Appl. Environ. Microbiol. 65:3386-3391.