Cryptosporidium parvum: PCR-RFLP analysis of the TRAP-C1 (thrombospondin-related adhesive protein of Cryptosporidium-1) gene discriminates between two alleles differentially associated with parasite isolates of animal and human origin

Experimental Parasitology

Volumn 90, Issue 2, 1998, Pages 195-198

  Spano, Furio ^a   Putignani, Lorenza ^a   Guida, Serena ^a   Crisanti, Andrea ^b  

^a SAPIENZA UNIVERSITY OF ROME   (Italy)

^b IMPERIAL COLLEGE LONDON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

THROMBOSPONDIN;

ALLELE; AMINO ACID SEQUENCE; ARTICLE; CRYPTOSPORIDIUM PARVUM; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN ISOLATION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; SPECIES DIFFERENCE;

ANIMALIA; CRYPTOSPORIDIUM; CRYPTOSPORIDIUM PARVUM;

EID: 0031684603     PISSN: 00144894     EISSN: None     Source Type: Journal    
DOI: 10.1006/expr.1998.4324     Document Type: Article

References (15)

1. Awad-El-Kariem, F. M., Robinson, H. A., Dyson, D. A., Evans, D., Wright, S., Fox, M. T., and McDonald, V. 1995. Differentiation between human and animal strains of Cryptosporidium parvum using isoenzyme typing. Parasitology 110, 129-132.
2. Bonnin, A., Fourmaux, M. N., Dubremetz, J. F., Nelson, R. G., Gobet, P., Harly, G., Biusson, M., Puygauthier-Toubas, D., Gabriel-Pospisil, F., Naciri, M., and Camerlynck, P. 1996. Genotyping human and bovine isolates of Cryptosporidium parvum by polymerase chain reaction-restriction fragment length polymorphism analysis of a repetitive DNA sequence. FEMS Microbiology Letters 137, 207-211.
3. Carraway, M., Tzipori, S., and Widmer, G. 1996. Identification of genetic heterogeneity in the Cryptosporidium parvum ribosomal repeat. Applied and Environmental Microbiology 62, 712-716.
4. Carraway, M., Tzipori, S., and Widmer, G. 1997. A new restriction fragment length polymorphism from Cryptosporidium parvum identifies genetically heterogeneous parasite populations and genotypic changes following transmission from bovine to human hosts. Infection and Immunity 65, 3958-3960.
5. Fayer, R., Speer, C. A., and Dubey, J. P. 1997. The general biology of Cryptosporidium. In "Cryptosporidium and Cryptosporidiosis" (R. Fayer, Ed.), pp. 65-92. CRC press, Boca Raton, PL.
6. Guerrant, R. L. 1997. Cryptosporidiosis: an emerging, highly infectious threat. Emerging Infectious Diseases 3, 1-10.
7. Graczyk, T. K., Fayer, R., and Cranfield, M. R. 1997. Zoonotic transmission of Cryptosporidium parvum: implications for water-borne cryptosporidiosis. Parasitology Today 9, 348-351.
8. Morgan, U. M., Constantine, C. C., O'Donoghue, P., Meloni, B. P., O'Brien, P. A., and Thompson, R. C. A. 1995. Molecular characterization of Cryptosporidium isolates from humans and other animals using random amplified polymorphic DNA analysis. American Journal of Tropical Medicine and Hygiene 52, 559-564.
9. Morgan, U. M., Constantine, C. C., Forbes, D. A., and Thompson, R. C. A. 1997. C. A. Differentiation between human and animal isolates of Cryptosporidium parvum using rDNA sequencing and direct PCR analysis. Journal of Parasitology 83, 825-830.
10. O'Donoghue, P. J. 1995. Cryptosporidium and Cryptosporidiosis in man and animals. International Journal for Parasitology 25, 139-195.
11. Peng, M. M., Xiao, L., Freeman, A. R., Arrowood, J., Escalante, A. A., Weltman, A. C., Ong, C. S. L., Mac Kenzie, W. R., Lal, A. A., and Beard, C. B. 1997. Genetic polymorphism among Cryptosporidium parvum isolates: evidence of two distinct human transmission cycles. Emerging Infectious Diseases 3, 567-573.
12. Smith, H. V., and Rose, J. B. 1990. Waterborne Cryptosporidiosis. Parasitology Today 6, 8-12.
13. Spano, F., Putignani, L., McLauchlin, J., Casemore, D. P., and Crisanti, A. 1997. PCR-RFLP analysis of the Cryptosporidium oocyst wall protein (COWP) gene discriminates between C. wrairi and C. parvum, and between C. parvum isolates of human and animal origin. FEMS Microbiology Letters 150, 209-217.
14. Spano, F., Putignani, L., Naitza, S., Puri, C., Wright, S., and Crisanti, A. 1998. Molecular cloning and expression analysis of a Cryptosporidium parvum gene encoding a new member of the thrombospondin family. Molecular and Biochemical Parasitology 92, 147-162.
15. Vasquez, J. R., Goozé, L., Kim, K., Gut, J., Petersen, C., and Nelson, R. G. 1996. Potential antifolate resistance determinants and genotypic variation in the bifunctional dihydrofolate reductase-thymidylate synthase gene from human and bovine isolates of Cryptosporidium parvum. Molecular and Biochemical Parasitology 79, 153-165.