Identification of the hepatitis C virus RNA replication complex in Huh-7 cells harboring subgenomic replicons

Journal of Virology

Volumn 77, Issue 9, 2003, Pages 5487-5492

  Gosert, Rainer ^a,c   Egger, Denise ^c   Lohmann, Volker ^b   Bartenschlager, Ralf ^b   Blum, Hubert E ^a   Bienz, Kurt ^c   Moradpour, Darius ^a  

^a UNIVERSITY OF FREIBURG   (Germany)

^b UNIVERSITY OF HEIDELBERG   (Germany)

^c UNIVERSITY OF BASEL   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

5 BROMOURIDINE 5' TRIPHOSPHATE; DACTINOMYCIN; UNCLASSIFIED DRUG; URIDINE DERIVATIVE; VIRUS PROTEIN; VIRUS RNA;

ARTICLE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; FLUORESCENCE IN SITU HYBRIDIZATION; GLYCOSYLATION; HEPATITIS C VIRUS; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; REPLICON; RNA REPLICATION; RNA SYNTHESIS; ULTRASTRUCTURE; VIRUS REPLICATION;

CELL MEMBRANE; HEPACIVIRUS; HUMANS; REPLICON; RNA, VIRAL; TUMOR CELLS, CULTURED; VIRAL NONSTRUCTURAL PROTEINS; VIRUS REPLICATION;

EID: 0345144016     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.77.9.5487-5492.2003     Document Type: Article

References (34)

1. Ahola, T., A. Lampio, P. Auvinen, and L. Kääriäinen. 1999. Semliki Forest virus mRNA capping enzyme requires association with anionic membrane phospholipids for activity. EMBO J. 18:3164-3172.
2. Bienz, K., and D. Egger. 1995. Immunocytochemistry and in situ hybridization in the electron microscope: combined application in the study of virus-infected cells. Histochem. Cell Biol. 103:325-338.
3. Bienz, K., D. Egger, and L. Pasamontes. 1987. Association of polioviral proteins of the P2 genomic region with the viral replication complex and virus-induced membrane synthesis as visualized by electron microscopic immunocytochemistry and autoradiography. Virology 160:220-226.
4. Bienz, K., D. Egger, T. Pfister, and M. Troxler. 1992. Structural and functional characterization of the poliovirus replication complex. J. Virol. 66:2740-2747.
5. Bienz, K., D. Egger, Y. Rasser, and W. Bossart. 1980. Kinetics and location of poliovirus macromolecular synthesis in correlation to virus-induced cytopathology. Virology 100:390-399.
6. Blight, K. J., A. A. Kolykhalov, and C. M. Rice. 2000. Efficient initiation of HCV RNA replication in cell culture. Science 290:1972-1974.
7. Brass, V., E. Bieck, R. Montserret, B. Wölk, J. A. Hellings, H. E. Blum, F. Penin, and D. Moradpour. 2002. An amino-terminal amphipathic alphahelix mediates membrane association of the hepatitis C virus nonstructural protein 5A. J. Biol. Chem. 277:8130-8139.
8. Egger, D., R. Gosert, and K. Bienz. 2002. Role of cellular structures in viral RNA replication, p. 247-253. In B. Semler and E, Wimmer (ed.), Molecular biology of picornaviruses. ASM Press, Washington, D.C.
9. Egger, D., B. Wölk, R. Gosert, L. Bianchi, H. E. Blum, D. Moradpour, and K. Bienz. 2002. Expression of hepatitis C virus proteins induces distinct membrane alterations including a candidate viral replication complex. J. Virol. 76:5974-5984.
10. Gosert, R., D. Egger, and K. Bienz. 2000. A cytopathic and a cell culture adapted hepatitis A virus strain differ in cell killing but not in intracellular membrane rearrangements. Virology 266:157-169.
11. Gosert, R., A. Kanjanahaluethai, D. Egger, K. Bienz, and S. C. Baker. 2002. RNA replication of mouse hepatitis virus takes place at double-membrane vesicles. J. Virol. 76:3697-3708.
12. Hügle, T., F. Fehrmann, E. Bieck, M. Kohara, H.-G. Kräusslich, C. M. Rice, H. E. Blum, and D. Moradpour. 2001. The hepatitis C virus nonstructural protein 4B is an integral endoplasmic reticulum membrane protein. Virology 284:70-81.
13. Ikeda, M., M. Yi, K. Li, and S. M. Lemon. 2002. Selectable subgenomic and genome-length dicistronic RNAs derived from an infectious molecular clone of the HCV-N strain of hepatitis C virus replicate efficiently in cultured Huh7 cells. J. Virol. 76:2997-3006.
14. Ivashkina, N., B. Wölk, V. Lohmann, R. Bartenschlager, H. E. Blum, F. Penin, and D. Moradpour. 2002, The hepatitis C virus RNA-dependent RNA polymerase membrane insertion sequence is a transmembrane segment. J. Virol. 76:13088-13093.
15. Krawczynski, K., M. J. Beach, D. W. Bradley, G. Kuo, A. M. Di Bisceglie, M. Houghton, G. R. Reyes, J. P. Kim, Q.-L. Choo, and M. J. Alter. 1992. Hepatitis C virus antigen in hepatocytes: immunomorphologic detection and identification. Gastroenterology 103:622-629.
16. Lau, J. Y. N., K. Krawczynski, F. Negro, and R. P. González-Peralta. 1996. In situ detection of hepatitis C virus - a critical appraisal. J. Hepatol. 24(Suppl. 2):43-51.
17. Lindenbach, B. D., and C. M. Rice. 2001. Flaviviridae: the viruses and their replication, p. 991-1041. In D. M. Knipe and P. M. Howley (ed.), Fields virology, 4th ed., vol. 1. Lippincott-Raven Publishers, Philadelphia, Pa.
18. Lohmann, V., F. Körner, A. Dobierzewska, and R. Bartenschlager. 2001. Mutations in hepatitis C virus RNAs conferring cell culture adaptation. J. Virol. 75:1437-1449.
19. Lohmann, V., F. Körner, J.-O. Koch, U. Herian, L. Theilmann, and R. Bartenschlager. 1999. Replication of subgenomic hepatitis C virus RNAs in a hepatoma cell line. Science 285:110-113.
20. Lyle, J. M., E. Bullitt, K. Bienz, and K. Kirkegaard. 2002. Visualization and functional analysis of RNA-dependent RNA polymerase lattices. Science 296:2218-2222.
21. Moradpour, D., V. Brass, R. Gosert, B. Wölk, and H. E. Blum. 2002. Hepatitis C: molecular virology and antiviral targets. Trends Mol. Med. 8:476-482.
22. Moradpour, D., P. Kary, C. M. Rice, and H. E. Blum. 1998. Continuous human cell lines inducibly expressing hepatitis C virus structural and non-structural proteins. Hepatology 28:192-201.
23. Pedersen, K. W., Y. van der Meer, N. Roos, and E. J. Snijder. 1999. Open reading frame 1a-encoded subunits of the arterivirus replicase induce endoplasmic reticulum-derived double-membrane vesicles which carry the viral replication complex. J. Virol. 73:2016-2026.
24. Pfeifer, U., R. Thomssen, K. Legler, U. Böttcher, W. Gerlich, E. Weinmann, and O. Klinge. 1980. Experimental non-A, non-B hepatitis: four types of cytoplasmic alteration in hepatocytes of infected chimpanzees. Virchows Arch. B Cell Pathol. Incl. Mol. Pathol. 33:233-243.
25. Pietschmann, T., V. Lohmann, A. Kaul, N. Krieger, G. Rinck, G. Rutter, D. Strand, and R. Bartenschlager. 2002. Persistent and transient replication of full-length hepatitis C virus genomes in cell culture. J. Virol. 76:4008-4021.
26. Pietschmann, T., V. Lohmann, G. Rutter, K. Kurpanek, and R. Bartenschlager. 2001. Characterization of cell lines carrying self-replicating hepatitis C virus RNAs. J. Virol. 75:1252-1264.
27. Schaad, M. C., P. E. Jensen, and J. C. Carrington. 1997. Formation of plant RNA virus replication complexes on membranes: role of an endoplasmic reticulum-targeted viral protein. EMBO J. 16:4049-4059.
28. Schmidt-Mende, J., E. Bieck, T. Hügle, F. Penin, C. M. Rice, H. E. Blum, and D. Moradpour. 2001. Determinants for membrane association of the hepatitis C virus RNA-dependent RNA polymerase. J. Biol. Chem. 276:44052-44063.
29. Schwartz, M., J. Chen, M. Janda, M. Sullivan, J. den Boon, and P. Ahlquist. 2002. A positive-strand RNA virus replication complex parallels form and function of retrovirus capsids. Mol. Cell 9:505-514,
30. Snijder, E. J., H. van Tol, N. Roos, and K. W. Pedersen. 2001. Non-structural proteins 2 and 3 interact to modify host cell membranes during the formation of the arterivirus replication complex. J. Gen. Virol. 82:985-994.
31. Teterina, N. L., D. Egger, K. Bienz, D. M. Brown, B. L. Semler, and E. Ehrenfeld. 2001. Requirements for assembly of poliovirus replication complexes and negative-strand RNA synthesis. J. Virol. 75:3841-3850.
32. Westaway, E. G., A. A. Khromykh, and J. M. Mackenzie. 1999. Nascent flavivirus RNA colocalized in situ with double-stranded RNA in stable replication complexes. Virology 258:108-117.
33. Wölk, B., D. Sansonno, H.-G. Kräusslich, F. Dammacco, C. M. Rice, H. E. Blum, and D. Moradpour. 2000. Subcellular localization, stability and transcleavage competence of the hepatitis C virus NS3-NS4A complex expressed in tetracycline-regulated cell lines. J. Virol. 74:2293-2304.
34. Wu, S.-X., P. Ahlquist, and P. Kaesberg. 1992, Active complete in vitro replication of nodavirus RNA requires glycerophospholipid. Proc. Natl. Acad. Sci. USA 89:11136-11140.