Phosphorylation-dependent Akt cleavage in neural cell in vitro reconstitution of apoptosis

Journal of Neurochemistry

Volumn 73, Issue 4, 1999, Pages 1773-1776

  François, Fleur ^a   Grimes, Mark L ^a  

^a MASSEY UNIVERSITY   (New Zealand)

Author keywords

Akt protein kinase B; Apoptosis; Caspase; In vitro reconstitution; Phosphorylation

Indexed keywords

ADENOSINE TRIPHOSPHATE; CASPASE 3; CASPASE INHIBITOR; CELL DNA; CYTOCHROME C; ISOENZYME; OKADAIC ACID; ORTHOVANADIC ACID; PROTEIN KINASE B;

ANIMAL CELL; APOPTOSIS; ARTICLE; CONTROLLED STUDY; DNA CLEAVAGE; ENZYME ACTIVATION; ENZYME ASSAY; HUMAN; HUMAN CELL; MOLECULAR DYNAMICS; NEUROBLASTOMA CELL; NONHUMAN; PHEOCHROMOCYTOMA CELL; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN PHOSPHORYLATION; RAT; REGULATORY MECHANISM; SIGNAL TRANSDUCTION;

ADENOSINE TRIPHOSPHATE; ANIMALS; APOPTOSIS; CASPASE 3; CASPASE 9; CASPASES; CELL NUCLEUS; CELL-FREE SYSTEM; CHROMATIN; CYTOCHROME C GROUP; CYTOSOL; ENZYME INHIBITORS; HUMANS; MODELS, NEUROLOGICAL; NEUROBLASTOMA; NEURONS; PC12 CELLS; PHOSPHORYLATION; PROTEIN-SERINE-THREONINE KINASES; PROTEIN-TYROSINE KINASES; PROTO-ONCOGENE PROTEINS; PROTO-ONCOGENE PROTEINS C-AKT; RATS; TUMOR CELLS, CULTURED;

EID: 0344994491     PISSN: 00223042     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1471-4159.1999.731773.x     Document Type: Article

References (20)

1. Cardone M. H., Roy N., Stennicke H. R., Salvesen G. S., Franke T. F., Stanbridge E., Frisch S., and Reed J. C. (1998) Regulation of cell death protease caspase-9 by phosphorylation. Science 282, 1318-1321.
2. Datta S. R., Dudek H., Tao X., Masters S., Fu H., Gotoh Y., and Greenberg M. E. (1997) Akt phosphorylation of BAD couples survival signals to the cell-intrinsic death machinery. Cell 91, 231-241.
3. Deshmukh M. and Johnson E. M. Jr. (1998) Evidence of a novel event during neuronal death: development of competence-to-die in response to cytoplasmic cytochrome c. Neuron 21, 695-705.
4. Dudek H., Datta S. R., Franke T. F., Birnbaum M. J., Yao R., Cooper G. M., Segal R. A., Kaplan D. R., and Greenberg M. E. (1997) Regulation of neuronal survival by the serine-threonine protein kinase Akt. Science 275, 661-665.
5. Enari M., Sakahira H., Yokoyama H., Okawa K., Iwamatsu A., and Nagata S. (1998) A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature 391, 43-50.
6. Greene L. A. and Tischler A. S. (1976) Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA 73, 2424-2428.
7. Hakem R., Hakem A., Duncan G. S., Henderson J. T., Woo M., Soengas M. S., Elia A., de la Pompa J. L., Kagi D., Khoo W., Potter J., Yoshida R., Kaufman S. A., Lowe S. W., Penninger J. M., and Mak T. W. (1998) Differential requirement for caspase 9 in apoptotic pathways in vivo. Cell 94, 339-352.
8. Jänicke R. U., Ng P., Sprengart M. L., and Porter A. G. (1998) Caspase-3 is required for alpha-fodrin cleavage but dispensable for cleavage of other death substrates in apoptosis. J. Biol. Chem. 273, 15540-15545.
9. Kuida K., Zheng T. S., Na S., Kuan C., Yang D., Karasuyama H., Rakic P., and Flavell R. A. (1996) Decreased apoptosis in the brain and premature lethality in CPP32-deficient mice. Nature 384, 368-372.
10. Lazebnik Y. A., Cole S., Cooke C. A., Nelson W. G., and Earnshaw W. C. (1993) Nuclear events of apoptosis in vitro in cell-free mitotic extracts: a model system for analysis of the active phase of apoptosis. J. Cell Biol. 123, 7-22.
11. Li P., Nijhawan D., Budihardjo I., Srinivasula S. M., Ahmad M., Alnemri E. S., and Wang X. (1997) Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell 91, 479-489.
12. Liu X., Kim C. N., Yang J., Jemmerson R., and Wang X. (1996) Induction of apoptotic program in cell-free extracts: requirement for dATP and cytochrome c. Cell 86, 147-157.
13. Messam C. A. and Pittman R. N. (1998) Asynchrony and commitment to die during apoptosis. Exp. Cell Res. 238, 389-398.
14. Oppenheim R. W. (1991) Cell death during development of the nervous system. Annu. Rev. Neurosci. 14, 453-501.
15. Philpott K. L., McCarthy M. J., Klippel A., and Rubin L. L. (1997) Activated phosphatidylinositol 3-kinase and Akt kinase promote survival of superior cervical neurons. J. Cell Biol. 139, 809-815.
16. Tilly J. L. and Hsueh A. J. (1993) Microscale autoradiographic method for the qualitative and quantitative analysis of apoptotic DNA fragmentation. J. Cell. Physiol. 154, 519-526.
17. Widmann C., Gibson S., and Johnson G. L. (1998) Caspase-dependent cleavage of signaling proteins during apoptosis. A turn-off mechanism for anti-apoptotic signals. J. Biol. Chem. 273, 7141-7147.
18. Yao R. and Cooper G. M. (1995) Requirement for phosphatidylinositol-3 kinase in the prevention of apoptosis by nerve growth factor. Science 267, 2003-2006.
19. Yoshida H., Kong Y. Y., Yoshida R., Elia A. J., Hakem A., Hakem R., Penninger J. M., and Mak T. W. (1998) Apaf1 is required for mitochondrial pathways of apoptosis and brain development. Cell 94, 739-750.
20. Zou H., Henzel W. J., Liu X., Lutschg A., and Wang X. (1997) Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3. Cell 90, 405-413.