A detection method for Legionella spp in (cooling) water: Fluorescent in situ hybridisation (FISH) on whole bacteria

Water Science and Technology

Volumn 47, Issue 3, 2003, Pages 143-146

  Declerck, P ^a   Verelst, L ^b   Duvivier, L ^b   Van Damme, A ^c   Ollevier, F ^a  

^a UNIVERSITY OF LEUVEN   (Belgium)

^b LABORELEC   (Belgium)

^c Electrabel   (Belgium)

Author keywords

Cooling waters; FISH; Legionella

Indexed keywords

FLUORESCENCE; MICROSCOPIC EXAMINATION; RNA; WATER FILTRATION;

FLUORESCENT IN SITU HYBRIDIZATION (FISH);

WATER BACTERIOLOGY;

COOLING WATER; RIBOSOME RNA;

BACTERIUM; COOLING WATER; DETECTION METHOD; MICROBIAL POLLUTANT; WATER QUALITY;

BACTERIAL STRAIN; BACTERIUM CULTURE; BACTERIUM DETECTION; CONFERENCE PAPER; CONTROLLED STUDY; FILTRATION; FLUORESCENCE IN SITU HYBRIDIZATION; FLUORESCENCE MICROSCOPY; GENE SEQUENCE; LEGIONELLA; LEGIONELLA PNEUMOPHILA; NONHUMAN; OLIGONUCLEOTIDE PROBE; RELIABILITY; RNA PROBE; SENSITIVITY AND SPECIFICITY; STAINING; WATER CONTAMINATION; WATER SAMPLING;

BACTERIA (MICROORGANISMS); LEGIONELLA; LEGIONELLA PNEUMOPHILA;

EID: 0344838356     PISSN: 02731223     EISSN: None     Source Type: Journal    
DOI: 10.2166/wst.2003.0184     Document Type: Conference Paper

References (7)

1. Glöckner, F.O., Amann, R., Alfreider, A., Pernthaler, J., Psenner, R., Trebesius, K.-H. and Schleifer, K.-H. (1996). An in situ hybridisation protocol for detection and identification of planctonic bacteria. System. Appl. Microbiol., 19, 403-406.
2. Glöckner, F.O., Fuchs, B.M. and Amann, R. (1999). Bacterioplankton compositions of lakes and oceans: A first comparison based on fluorescence in situ hybridisation. Appl. Environ. Microbiol., 65(8), 3721-3726.
3. Grimm, D., Merkert, H., Ludwig, W., Schleifer, K.-H., Hacker, J. and Brand, B.C. (1998). Specific detection of Legionella pneumophila: Construction of a new 16S rRNA-targeted oligonucleotide probe. Appl. Environ. Microbiol., 64(7), 2686-2690.
4. Manz, W., Amann, R., Szewzyk, R., Swewzyk, U., Stenström, T.A., Hutzler, P. and Schleifer, K.-H. (1995). In situ identification of Legionellaceae using 16S rRNA-targeted oligonucleotide probes and confocal laser scanning microscopy. Microbiol., 141, 29-39.
5. Moter, A. and Göbel, U.B. (2000). Fluorescence in situ hybridisation (FISH) for direct visualization of microorganisms. J. Microbiol. Meth., 41, 85-112.
6. Sherr, E.B., Caron, D.A. and Sherr, B.F. (1993). Staining of heterotrophic protists for visualization via epifluorescence microscopy. In "Handbook of Methods in Aquatic Microbial Ecology" Lewis Publishers, pp. 213-228.
7. Wagner, M., Schmid, M., Juretschko, S., Trebesius, K.-H., Bubert, A., Goebel, W. and Schleifer, K.-H. (1998). In situ detection of a virulence factor mRNA and 16S rRNA in Listeria monocytogenes. FEMS Microbiol. Lett., 160, 159-168.