Low Concentrations of Ethanol Induce Apoptosis in Human Intestinal Cells

Scandinavian Journal of Gastroenterology

Volumn 38, Issue 11, 2003, Pages 1154-1161

  Asai, K ^b   Buurman, W A ^a   Reutelingsperger, C P M ^b   Schutte, B ^b   Kaminishi, M ^b  

^a MAASTRICHT UNIVERSITY   (Netherlands)

^b NONE

Author keywords

Annexin V; Apoptosis; Caspases; DNA fragmentation; Ethanol; Human intestinal epithelial Caco 2 cell

Indexed keywords

ALCOHOL; CASPASE; CASPASE INHIBITOR; CYTOKERATIN 18; DNA FRAGMENT; ENDOTOXIN; FLUORESCEIN ISOTHIOCYANATE; LIPOCORTIN 5; PHOSPHATIDYLSERINE;

ALCOHOL CONSUMPTION; APOPTOSIS; ARTICLE; CELL STRAIN CACO 2; CHRONIC LIVER DISEASE; CONCENTRATION RESPONSE; CONTROLLED STUDY; CYTOSKELETON; ENDOTOXEMIA; ENZYME INHIBITION; HUMAN; HUMAN CELL; INTESTINE CELL; MICROTUBULE ASSEMBLY; PRIORITY JOURNAL; TIGHT JUNCTION;

ADENOCARCINOMA; APOPTOSIS; CACO-2 CELLS; CASPASES; COLONIC NEOPLASMS; DNA FRAGMENTATION; DOSE-RESPONSE RELATIONSHIP, DRUG; ENZYME ACTIVATION; EPITHELIAL CELLS; ETHANOL; HUMANS; INTESTINES; PHOSPHATIDYLSERINES; SOLVENTS; TUMOR CELLS, CULTURED;

EID: 0344254804     PISSN: 00365521     EISSN: None     Source Type: Journal    
DOI: 10.1080/00365520310006252     Document Type: Article

References (40)

1. Hayton WL. Rate-limiting barriers to intestinal drug absorption: a review. J Pharmacokinet Biophar 1980;8:321-34.
2. Madara JL. Warner-Lambert/Parke-Davis Award lecture. Pathobiology of the intestinal epithelial barrier. Am J Pathol 1990;137:1273-81.
3. Hollander D. Intestinal permeability, leaky gut, and intestinal disorders. Curr Gastroenterol Rep 1999;1:410-6.
4. Nolan JP. The role of endotoxin in liver injury. Gastroenterology 1975;69:1346-56.
5. Raetz CR, Ulevitch RJ, Wright SD, Sibley CH, Ding A, Nathan CF. Gram-negative endotoxin: an extraordinary lipid with profound effects on eukaryotic signal transduction. FASEB J 1991;5:2652-60.
6. Bode C, Kugler V, Bode JC. Endotoxemia in patients with alcoholic and non-alcoholic cirrhosis and in subjects with no evidence of chronic liver disease following acute alcohol excess. J Hepatol 1987;4:8-14.
7. Lumsden AB, Henderson JM, Kutner MH. Endotoxin levels measured by a chromogenic assay in portal, hepatic and peripheral venous blood in patients with cirrhosis. Hepatology 1988;8:232-6.
8. Felver ME, Mezey E, McGuire M, Mitchell MC, Herlong HF, Veech GA. Plasma tumor necrosis factor alpha predicts decreased long-term survival in severe alcoholic hepatitis. Alcohol Clin Exp Res 1990;14:255-9.
9. Schenker S, Bay MK. Alcohol and endotoxin: another path to alcoholic liver injury? Alcohol Clin Exp Res 1995;19:1364-6.
10. Fujimoto M, Uemura M, Nakatani Y, Tsujita S, Hoppo K, Tamagawa T, et al. Plasma endotoxin and serum cytokine levels in patients with alcoholic hepatitis: relation to severity of liver disturbance. Alcohol Clin Exp Res 2000;24:48S-54S.
11. Adachi Y, Moore LE, Bradford BU, Gao W, Thurman RG. Antibiotics prevent liver injury in rats following long-term exposure to ethanol. Gastroenterology 1995;108:218-24.
12. Nanji AA, Khettry U, Sadrzadeh SM. Lactobacillus feeding reduces endotoxemia and severity of experimental alcoholic liver. Proc Soc Exp Biol Med 1994;205:243-7.
13. Robinson GM, Orrego H, Israel Y, Devenyi P, Kapur BM. Low-molecular-weight polyethylene glycol as a probe of gastrointestinal permeability after alcohol ingestion. Dig Dis Sci 1981;26:971-7.
14. Bjarnason I, Peters TJ, Wise RJ. The leaky gut of alcoholism: possible route of entry for toxic compounds. Lancet 1984;1:179-82.
15. Keshavarzian A, Holmes EW, Patel M, Iber F, Fields JZ, Pethkar S. Leaky gut in alcoholic cirrhosis: a possible mechanism for alcohol-induced liver damage. Am J Gastroenterol 1999;94:200-7.
16. Barona E, Pirola RC, Leiber CS. Small intestinal damage and changes in cell population produced by ethanol ingestion in the rat. Gastroenterology 1974;66:226-34.
17. Gottfried EB, Korsten MA, Lieber CS. Alcohol-induced gastric and duodenal lesions in man. Am J Gastroenterol 1978;70:587-92.
18. Tarnawski A, Hollander D, Stachura J, Klimczyk B, Mach T, Bogdal J. Alcohol injury to the normal human gastric mucosa: endoscopic, histologic and functional assessment. Clin Invest Med 1987;10:259-63.
19. Millan MS, Morris GP, Beck IT, Henson JT. Villous damage induced by suction biopsy and by acute ethanol intake in normal human small intestine. Dig Dis Sci 1980;25:513-25.
20. Ma TY, Nguyen D, Bui V, Nguyen H, Hoa N. Ethanol modulation of intestinal epithelial tight junction barrier. Am J Physiol 1999;276:G965-74.
21. Banan A, Choudhary S, Zhang Y, Fields JZ, Keshavarzian A. Ethanol-induced barrier dysfunction and its prevention by growth factors in human intestinal monolayers: evidence for oxidative and cytoskeletal mechanisms. J Pharmacol Exp Ther 1999;291:1075-85.
22. Hidalgo IJ, Raub TJ, Borchardt RT. Characterization of the human colon carcinoma cell line (Caco-2) as a model system for intestinal epithelial permeability. Gastroenterology 1989;96:736-49.
23. Meunier V, Bourrie M, Berger Y, Fabre G. The human intestinal epithelial cell line Caco-2; pharmacological and pharmacokinetic applications. Cell Biol Toxicol 1995;11:187-94.
24. Koopman G, Reutelingsperger CP, Kuijten GA, Keehnen RM, Pals ST, van Oers MH. Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis. Blood 1994;84:1415-20.
25. Nicoletti I, Migliorati G, Pagliacci MC, Grignani F, Riccardi C. A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. J Immunol Methods 1991;139:271-9.
26. Leers MP, Kolgen W, Bjorklund V, Bergman T, Tribbick G, Persson B, et al. Immunocytochemical detection and mapping of a cytokeratin 18 neo-epitope exposed during early apoptosis. J Pathol 1999;187:567-72.
27. Bretscher MS. Asymmetrical lipid bilayer structure for biological membranes. Nat New Biol 1972;236:11-2.
28. van Engeland M, Nieland LJ, Ramaekers FC, Schutte B, Reutelingsperger CP. Annexin V-affinity assay: a review on an apoptosis detection system based on phosphatidylserine exposure. Cytometry 1998;31:1-9.
29. Martin SJ, Cotter TG. Specific loss of microtubules in HL-60 cells leads to programmed cell death (apoptosis). Biochem Soc Trans 1990;18:299-301.
30. Janmey PA. The cytoskeleton and cell signaling: component localization and mechanical coupling. Physiol Rev 1998;78:763-81.
31. Sun Z, Wang X, Wallen R, Deng X, Du X, Hallberg E, et al. The influence of apoptosis on intestinal barrier integrity in rats. Scand J Gastroenterol 1998;33:415-22.
32. Bojarski C, Bendfeldt K, Gitter AH, Mankertz J, Fromm M, Wagner S, et al. Apoptosis and intestinal barrier function. Ann N Y Acad Sci 2000;915:270-4.
33. Bojarski C, Gitter AH, Bendfeldt K, Mankertz J, Schmitz H, Wagner S, et al. Permeability of human HT-29/B6 colonic epithelium as a function of apoptosis. J Physiol 2001;535:541-52.
34. Dive C, Gregory CD, Phipps DJ, Evans DL, Milner AE, Wyllie AH. Analysis and discrimination of necrosis and apoptosis (programmed cell death) by multiparameter flow cytometry. Biochim Biophys Acta 1992;1133:275-85.
35. Wyllie AH, Kerr JF, Currie AR. Cell death: the significance of apoptosis. Int Rev Cytol 1980;68:251-306.
36. Kerr JFR, Harmon BV. Definition and incidence of apoptosis: an historical prespective. In: Tomei LD, Cope F, editors. Apoprosis: the molecular basis of cell death. Cold Spring Harbor: Cold Spring Harbor Laboratory Press; 1991. p. 1-26.
37. Pelletier G, Briantais MJ, Buffet C, Pillot J, Etienne JP. Serum and intestinal secretory IgA in alcoholic cirrhosis of the liver. Gut 1982;23:475-9.
38. Diebel LN, Liberati DM, Dulchavsky SA, Diglio CA, Brown WJ. Ethanol impairs intestinal barrier defense by modulation of immunoglobulin. A transport. Surgery 2002;132:573-81.
39. Uesugi T, Froh M, Arteel GE, Bradford BU, Wheeler MD, Gabele E, et al. Role of lipopolysaccharide-binding protein in early alcohol-induced liver injury in mice. J Immunol 2002;168:2963-9.
40. Yin M, Bradford BU, Wheeler MD, Uesugi T, Froh M, Goyert SM, Thurman RG. Reduced early alcohol-induced liver injury in CD14-deficient mice. J Immunol 2001;166:4737-42.