A novel high throughput screening assay for HCV NS3 helicase activity

Antiviral Research

Volumn 46, Issue 3, 2000, Pages 181-193

  Hicham Alaoui Ismaili, Moulay ^a   Gervais, Christian ^a   Brunette, Stephanie ^a   Gouin, Genevieve ^a   Hamel, Martine ^a   Rando, Robert F ^a   Bedard, Jean ^a  

^a Shire Canada   (Canada)

Author keywords

Flashplate(TM); HCV NS3 helicase; High throughput screening assay

Indexed keywords

CATION; EDETIC ACID; HELICASE;

ARTICLE; ENZYME ACTIVITY; ENZYME ASSAY; HEPATITIS C VIRUS; PH; PRIORITY JOURNAL; SCREENING; SCREENING TEST;

ANIMALS; CATIONS, DIVALENT; DNA; EDETIC ACID; ENZYME INHIBITORS; HEPACIVIRUS; RECOMBINANT PROTEINS; REPRODUCIBILITY OF RESULTS; VIRAL NONSTRUCTURAL PROTEINS;

ESCHERICHIA COLI; HEPATITIS C VIRUS;

EID: 0342859314     PISSN: 01663542     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-3542(00)00085-1     Document Type: Article

References (31)

1. Bartenschlager, R., 1997a. Molecular targets in inhibition of hepatitis C virus replication. Antiviral Chem. Chemother. 8, 281-301.
2. Bartenschlager, R., 1997b. Candidate targets for hepatitis C virus-specific antiviral therapy. Intervirology 40, 378-393.
3. Bird, L.E., Subramanya, H.S., Wigley, D.B., 1998. Helicases: a unifying structural theme? Curr. Opin. Struct. Biol. 8, 14-18.
4. Cho, H.S., Ha, N.C., Kang, L.W., Chung, K.M., Back, S.H., Jang, S.K., Oh, B.H., 1998. Crystal structure of RNA helicase from genotype 1b hepatitis C virus. A feasible mechanism of unwinding duplex RNA. J. Biol. Chem. 273, 15045-15052.
5. Choo, Q.L., Kuo, G., Weiner, A.J., Overby, L.R., Bradley, D.W., Houghton, M., 1989. Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome. Science 244, 359-362.
6. Clarke, B., 1997. Molecular virology of hepatitis C virus. J. Gen. Virol. 78, 2397-2410.
7. Dhillon, A.P., Dusheiko, G.M., 1995. Pathology of hepatitis C virus infection. Histopathology 26, 297-309.
8. Faila, C., Tomei, L., de Francesco, R., 1994. Both NS3 and NS4A are required for proteolytic processing of hepatitis C virus nonstructural proteins. J. Virol. 68, 3753-3760.
9. Gallinari, P., Brennan, D., Nadri, A., Brunetti, M., Tomei, L., Steinkuhler, C., De Francesco, R., 1998. Multiple enzymatic activities associated with recombinant NS3 protein of hepatitis C virus. J. Virol. 72, 6758-6769.
10. Houghton, M., 1996. Hepatitis C viruses. In: Fields, B.N., Knipe, D.M., Howley, P.M., et al. (Eds.), Virology, 3rd ed. Lippincott-Raven, Philadelphia, PA, pp. 1035-1058.
11. Hsu, C.C., Hwang, L.H., Huang, Y.W., Chi, W.K., Chu, Y.D., Chen, D.S., 1998. An ELISA for RNA helicase activity: application as an assay of the NS3 helicase of hepatitis C virus. Biochem. Biophys. Res. Commun. 253, 594-599.
12. Iwarson, S., 1994. The natural course of chronic hepatitis C. FEMS Microbiol. Rev. 14, 201-204.
13. Kadare, G., Haenni, A.L., 1997. Virus-encoded RNA helicases. J. Virol. 71, 2583-2590.
14. Kew, M.C., 1994. Hepatitis C virus and hepatocellular carcinoma. FEMS Microbiol. Rev. 14, 211-220.
15. Kim, D.W., Gwack, Y., Han, J.H., Choe, J., 1997. Towards defining a minimal domain for NTPase and RNA helicase activities of the hepatitis C virus NS3 protein. Virus Res. 49, 17-25.
16. Kim, J.L., Morgenstern, K.A., Griffith, J.P., Dwyer, M.D., Thompson, J.A., Murcko, M.A., Lin, C., Caron, P.R., 1998. Hepatitis C virus NS3 RNA helicase domain with a bound oligonucleotide: the crystal structure provides insights into the mode of unwinding. Structure 6, 89-100.
17. Korolev, S., Yao, N., Lohman, T.M., Weber, P.C., Waksman, G., 1998. Comparisons between the structures of HCV and Rep helicases reveal structural similarities between SF1 and SF2 super-families of helicases. Protein Sci. 7, 605-610.
18. Kuo, G., Choo, Q.L., Alter, H.J., Gitnick, G.L., Redecker, A.G., Purcell, R.H., Myamura, T., Dienstag, J.L., Alter, M.J., Styevens, C.E., Tagtmeyer, G.E., Bonino, F., Colombo, M., Lee, W.S., Kuo, C., Berger, K., Shister, J.R., Overby, L.R., Bradley, D.W., Houghton, M., 1989. An assay for circulating antibodies to a major ethiologic virus of human non-A, non-B hepatitis. Science 244, 362-364.
19. Kwong, A.D., Risano, C., 1999. Development of a hepatitis C virus RNA helicase high throughput assay. In: Kinchington, D., Schinazi, R.F. (Eds.), Antiviral Methods and Protocols. Humana Press, Totowa, NJ, pp. 97-116.
20. Kyono, K., Miyashiro, M., Taguchi, I., 1998. Detection of hepatitis C virus helicase activity using the scintillation proximity assay system. Anal. Biochem. 257, 120-126.
21. Lohmann, V., Körner, F., Koch, J.-O., Herian, U., Theilmann, L., Bartenschlager, R., 1999. Replication of subgenomic hepatitis C virus RNAs in a hepatoma cell line. Science 285, 110-113.
22. Lüking, A., Stahl, U., Schmidt, U., 1998. The protein family of RNA helicases. Crit. Rev. Biochem. Mol. Biol. 33, 259-296.
23. Morgenstern, K.A., Landro, J.A., Hsiao, K., Lin, C., Gu, Y., Su, M.S., Thompson, J.A., 1997. Polynucleotide modulation of the protease, nucleoside triphosphatase, and helicase activities of a hepatitis C virus NS3-NS4A complex isolated from transfected COS cells. J. Virol. 71, 3767-3775.
24. Nakajima, T., Urchida, C., Anderson, S.F., Lee, C.G., Hurwitz, J., Parvin, J.D., Montminy, M., 1997. RNA helicase A mediates association of CBP with RNA polymerase II. Cell 90, 1107-1112.
25. Raney, K.D., Sowers, L.C., Millar, D.P., Benkovic, S.J., 1994. A fluorescence-based assay for monitoring helicase activity. Proc. Natl. Acad. Sci. USA 91, 6644-6648.
26. Rice, C.M., 1996. Flaviviridae: the viruses and their replication. In: Fields, B.N., Knipe, D.M., Howley, P.M., et al. (Eds.), Virology, 3rd ed. Lippincott-Raven, Philadelphia, PA, pp. 931-959.
27. Saito, I., Miyamura, T., Ohbayashi, A., Harada, H., Katayama, T., Kikuchi, S., Watanabe, T.Y., Koi, S., Onji, M., Ohta, Y., et al., 1990. Hepatitis C virus infection is associated with the development of hepatocellular carcinoma. Proc. Natl. Acad. Sci. USA 87, 6547-6549.
28. Sivaraja, M., Giordano, H., Peterson, M.G., 1998. High-throughput screening assay for helicase enzymes. Anal. Biochem. 265, 22-27.
29. Tomei, L., Faila, C., Santolini, E., de Francesco, R., La Monica, N., 1993. NS3 is a serine protease required for processing of hepatitis C virus polyprotein. J. Virol. 67, 4017-4026.
30. Weiland, O., 1994. Interferon therapy in chronic hepatitis C virus infection. FEMS Microbiol. Rev. 14, 279-288.
31. Yao, N., Hesson, T., Cable, M., Hong, Z., Kwong, A.D., Le, H.V., Weber, P.C., 1997. Structure of the hepatitis C virus RNA helicase domain. Nat. Struct. Biol. 4, 463-467.