Characterization of receptor-mediated [35S]GTPγS binding to cortical membranes from postmortem human brain

European Journal of Pharmacology

Volumn 390, Issue 1-2, 2000, Pages 25-36

  González Maeso, Javier ^a   Rodríguez Puertas, Rafael ^a   Gabilondo, Ane M ^a   Meana, J Javier ^a  

^a UNIVERSITY OF THE BASQUE COUNTRY UPV EHU   (Spain)

Author keywords

5 HT1A receptor; Brain; G protein; human; 35S GTP S binding; 2 Adrenoceptor; Opioid receptor

Indexed keywords

2 (2 METHOXY 1,4 BENZODIOXAN 2 YL) 2 IMIDAZOLINE; 2 DIPROPYLAMINO 8 HYDROXYTETRALIN; 4 AMINOBUTYRIC ACID B RECEPTOR; ALKYLATING AGENT; ALPHA 2 ADRENERGIC RECEPTOR; ALPHA 2 ADRENERGIC RECEPTOR STIMULATING AGENT; ATROPINE; BACLOFEN; BETHANECHOL; BRIMONIDINE; CARBACHOL; ENKEPHALIN[2 DEXTRO ALANINE 4 METHYLPHENYLALANINE 5 GLYCINE]; GUANINE NUCLEOTIDE BINDING PROTEIN; GUANOSINE 5' O (3 THIOTRIPHOSPHATE); GUANOSINE DIPHOSPHATE; INHIBITORY GUANINE NUCLEOTIDE BINDING PROTEIN; MAGNESIUM ION; MORPHINE; MU OPIATE RECEPTOR; MUSCARINIC RECEPTOR; N [2 [4 (2 METHOXYPHENYL) 1 PIPERAZINYL]ETHYL] N (2 PYRIDYL)CYCLOHEXANECARBOXAMIDE; N ETHYLMALEIMIDE; NALOXONE; PHACLOFEN; PROTEIN; SEROTONIN 1A RECEPTOR; SODIUM CHLORIDE; SULFUR 35;

ADULT; AGONIST; ARTICLE; AUTOPSY; AUTORADIOGRAPHY; BINDING AFFINITY; BRAIN HOMOGENATE; BRAIN MEMBRANE; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG ANTAGONISM; DRUG POTENTIATION; DRUG RECEPTOR BINDING; GRAY MATTER; HUMAN; HUMAN TISSUE; MALE; PREFRONTAL CORTEX; PRIORITY JOURNAL; RECEPTOR AFFINITY; RECEPTOR DENSITY;

ADRENERGIC ALPHA-AGONISTS; AUTORADIOGRAPHY; CEREBRAL CORTEX; ETHYLMALEIMIDE; GTP-BINDING PROTEINS; GUANOSINE 5'-O-(3-THIOTRIPHOSPHATE); GUANOSINE DIPHOSPHATE; HUMANS; MEMBRANES; RECEPTORS, ADRENERGIC, ALPHA-2; SODIUM CHLORIDE; STIMULATION, CHEMICAL; SULFHYDRYL REAGENTS; SULFUR RADIOISOTOPES; TIME FACTORS;

EID: 0342632832     PISSN: 00142999     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0014-2999(99)00827-4     Document Type: Article

References (63)

1. m GTPase by inhibitors of adipocyte adenylate cyclase. Mol. Pharmacol. 21:1981;336-342.
2. 35S]GTPγS binding in rat hippocampal membranes. Eur. J. Pharmacol. 343:1998;303-312.
3. Asano T., Ogasawara N. Uncoupling of γ-aminobutyric acid B receptors from GTP-binding proteins by N-ethylmaleimide: effect of N-ethylmaleimide on purified GTP-binding proteins. Mol. Pharmacol. 29:1986;244-249.
4. Birnbaumer L., Abramowitz J., Brown A.M. Receptor-effector coupling by G proteins. Biochim. Biophys. Acta. 1031:1990;163-224.
5. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:1976;248-254.
6. 35S]GTPγS binding to rat cerebellar membranes correlates with agonist-induced decreases in GDP affinity. J. Biol. Chem. 273:1998;16865-16873.
7. Breivogel C.S., Sim L.J., Childers S.R. Regional differences in cannabinoid receptor/G-protein coupling in rat brain. J. Pharmacol. Exp. Ther. 282:1997;1632-1642.
8. Bruns R.F., Lawson-Wendling K., Pugsley T.A. A rapid filtration assay for soluble receptors using polyethylenimine-treated filters. Anal. Biochem. 132:1983;74-81.
9. 2A-adrenoceptor agonist binding sites in brain of depressed suicide victims. J. Neurochem. 70:1998;1114-1123.
10. 2-adrenergic receptor with guanine nucleotide regulatory proteins in phospholipid vesicles. J. Biol. Chem. 261:1986;3901-3909.
11. Clapham D.E., Neer E.J. G protein βγ subunits. Annu. Rev. Pharmacol. Toxicol. 37:1997;167-203.
12. 35S]GTPγS binding shows regional selective changes in Alzheimer's disease postmortem brain. Neurodegeneration. 5:1996;19-26.
13. m GTPase activity in aging and Alzheimer's disease: implications for altered signal transduction. Brain Res. 664:1994;54-60.
14. De Paermentier F., Mauger J.M., Lowther S., Crompton M.R., Katona C.L.E., Horton R.W. Brain α-adrenoceptors in depressed suicides. Brain Res. 757:1997;60-68.
15. 35S]GTPγS binding. J. Neurochem. 70:1998;1258-1268.
16. 35S]GTPγS-binding responses in the human and monkey brain. Eur. J. Neurosci. 11:1999;1809-1817.
17. Ferrari-DiLeo G., Mash D.C., Flynn D.D. Attenuation of muscarinic receptor-G-protein interaction in Alzheimer disease. Mol. Chem. Neurophatol. 24:1995;69-91.
18. Friedman E., Wang H.-Y. Receptor-mediated activation of G proteins is increased in postmortem brains of bipolar affective disorder subjects. J. Neurochem. 67:1996;1145-1152.
19. 2-adrenoceptor agonist binding sites in the postmortem brain of heroin addicts. Psychopharmacology. 115:1994;135-140.
20. + regulation of formyl peptide receptor-mediated signal transduction in HL 60 cells. Evidence that the cation prevents activation of the G-protein by unoccupied receptors. Eur. J. Pharmacol. 172:1989;481-492.
21. Gilman A.G. G proteins: transducers of receptor-generated signals. Annu. Rev. Biochem. 56:1987;615-649.
22. 2-adrenoceptor agonist binding sites in the hippocampus and frontal cortex of depressed suicide victims. J. Neurochem. 63:1994;256-265.
23. 35S]GTPγS binding in postmortem human brain. Eur. J. Neurosci. 10(Suppl. 10):1998;290.
24. 3H]-RX821002 binding to membranes and tissue sections. Eur. J. Pharmacol. 310:1996;83-93.
25. 3H]8-Hydroxy-2-(di-n-propylamino)tetralin binding to pre- and postsynaptic 5-hydroxytryptamine sites in various regions of the rat brain. J. Neurochem. 44:1985;1685-1696.
26. Happe H.K., Bylund D.B., Murrin L.C. Alpha-2 adrenergic receptor functional coupling to G proteins in rat brain during postnatal development. J. Pharmacol. Exp. Ther. 288:1999;1134-1142.
27. Hilf G., Gierschik P., Jakobs K.H. Muscarinic acetylcholine receptor-stimulated binding of guanosine 5′-O-(3-thiotriphosphate) to guanine-nucleotide-binding proteins in cardiac membranes. Eur. J. Biochem. 186:1989;725-731.
28. 2-adrenergic receptors by sodium. J. Biol. Chem. 265:1990;21590-21595.
29. 2 adrenergic receptors. Biochem. Pharmacol. 55:1998;1035-1043.
30. Katada T., Northup J.K., Bokoch G.M., Ui M., Gilman A.G. The inhibitory guanine nucleotide-binding regulatory component of adenylate cyclase. J. Biol. Chem. 259:1984;3578-3585.
31. 3 receptor-dependent G protein activation in rat brain tissue sections. J. Neurochem. 71:1998;808-816.
32. 35S]-GTPγS binding mediated by human muscarinic m1-m4 receptors: antagonists studies. Br. J. Pharmacol. 109:1993;1120-1127.
33. 35S]thio)triphosphate binding. Mol. Pharmacol. 44:1993;115-123.
34. Manji H.K. G proteins: implications for psychiatry. Am. J. Psychiatry. 149:1992;746-760.
35. McPherson G.A. Analysis of radioligand binding experiments: a collection of computer programs for IBM PC. J. Pharmacol. Methods. 14:1985;213-228.
36. 3H]UK14304. J. Neurochem. 52:1989;1210-1217.
37. 2-Adrenoceptors in the brain of suicide victims: increased receptor density associated with major depression. Biol. Psychiatry. 31:1992;471-490.
38. 2-adrenoceptors in postmortem brains from patients with Alzheimer's disease. J. Neurochem. 58:1992;1896-1904.
39. + and guanine nucleotides. Nature. 288:1980;709-711.
40. Munson P.J., Rodbard D. LIGAND: a versatile computerized approach for characterization of ligand binding system. Anal. Biochem. 107:1980;220-239.
41. 1A receptors. Eur. J. Pharmacol. 307:1996;107-111.
42. 1A receptors as a function of receptor:G-protein stoichiometry. Neuropharmacology. 36:1997;451-459.
43. Odagaki Y., Nishi N., Ozawa H., Saito T., Takahata N., Riederer P., Koyama T. Measurement of receptor-mediated functional activation of G proteins in postmortem human brain membranes. Brain Res. 789:1998;84-91.
44. 35S]thiotriphosphate) binding by cholinergic muscarinic receptors in membranes of rat olfactory bulb. J. Neurochem. 67:1996;2549-2556.
45. 2-adrenoceptors is elevated in the locus coeruleus from victims of suicide. J. Neurochem. 63:1994;617-624.
46. Pacheco M.A., Stockmeier C., Meltzer H.Y., Overholser J.C., Dilley G.E., Jope R.S. Alterations in phosphoinositide signalling and G-protein levels in depressed suicide brain. Brain Res. 723:1996;37-45.
47. 2-adrenoceptors. Eur. J. Pharmacol. 355:1998;275-279.
48. Pert C.B., Snyder S.H. Opiate receptor binding of agonists and antagonists affected differentially by sodium. Mol. Pharmacol. 10:1974;868-879.
49. Rodríguez-Puertas R., González-Maeso J., Meana J.J., Pazos A. Visualization of receptor-activated G proteins in postmortem human brain. Soc. Neurosci. 24:1998;603.
50. + and guanyl nucleotides. J. Biol. Chem. 255:1980;820-823.
51. Sastre M., García-Sevilla J.A. Density of alpha-2A adrenoceptors and Gi proteins in the human brain: ratio of high-affinity agonist sites to antagonist sites and effect of age. J. Pharmacol. Exp. Ther. 269:1994;1062-1072.
52. Selley D.E., Sim L.J., Xiao R., Liu Q., Childers S.R. μ-Opioid receptor-stimulated guanosine-5′-O-(γ-thio)-triphosphate binding in rat thalamus and cultured cell lines: signal transduction mechanisms underlying agonist efficacy. Mol. Pharmacol. 51:1997;87-96.
53. 35S]thio]-triphosphate binding. Proc. Natl. Acad. Sci. U. S. A. 92:1995;7242-7246.
54. Sim L.J., Selley D.E., Xiao R., Childers S.R. Differences in G-protein activation by μ- and δ-opioid, and cannabinoid, receptors in rat striatum. Eur. J. Pharmacol. 307:1996;97-105.
55. Sternweis P.C., Robishaw J.D. Isolation of two proteins with high affinity for guanine nucleotides from membranes of bovine brain. J. Biol. Chem. 259:1984;13806-13813.
56. 2-adrenergic receptor activation of G proteins: evidence for a precoupled receptor/G protein state. Mol. Pharmacol. 45:1994;524-531.
57. 35S]thio)triphosphate binding to membranes from human neuroblastoma SH-SY5Y cells. Mol. Pharmacol. 47:1995;848-854.
58. o with μ-opioid receptors in guinea pig striatal membranes pretreated with micromolar concentrations of N-ethylmaleimide. J. Neurochem. 54:1990;841-848.
59. 35S]guanosine-5′-O-(3-thio)triphosphate binding to rodent brain sections as visualized by in vitro autoradiography. Mol. Pharmacol. 52:1997;623-631.
60. Wang H.-Y., Friedman E. Receptor-mediated activation of G proteins is reduced in postmortem brains from Alzheimer's disease patients. Neurosci. Lett. 173:1994;37-39.
61. Wieland T., Jakobs K.H. Measurement of receptor-stimulated guanosine 5′-O-(γ-thio)triphosphate binding by G proteins. Methods Enzymol. 237:1994;3-13.
62. 35S]guanosine-5′-O-(3-thio)triphosphate binding: agonist potencies and the influence of sodium chloride on intrinsic activity. Mol. Pharmacol. 51:1997;1060-1069.
63. sα protein levels and forskolin-stimulated cyclic AMP formation are increased in bipolar affective disorder. J. Neurochem. 61:1993;890-898.