Subtype-specific stimulation of [35S]GTPγS binding by recombinant α2-adrenoceptors

European Journal of Pharmacology

Volumn 355, Issue 2-3, 1998, Pages 275-279

  Peltonen, Juha M ^a   Pihlavisto, Marjo ^a   Scheinin, Mika ^a,b  

^a UNIVERSITY OF TURKU   (Finland)

^b UNIVERSITY OF TURKU   (Finland)

Author keywords

2 Adrenoceptor subtype; Efficacy; G protein; Guanosine 5 O thiotriphosphate; Potency

Indexed keywords

ALPHA 2 ADRENERGIC RECEPTOR; ALPHA 2A ADRENERGIC RECEPTOR; ALPHA 2C ADRENERGIC RECEPTOR; ALPHA ADRENERGIC RECEPTOR; ALPHA ADRENERGIC RECEPTOR BLOCKING AGENT; ALPHA ADRENERGIC RECEPTOR STIMULATING AGENT; ATIPAMEZOLE; BRIMONIDINE; CLONIDINE; DEXMEDETOMIDINE; GUANINE NUCLEOTIDE BINDING PROTEIN; GUANOSINE 5' O (3 THIOTRIPHOSPHATE); NORADRENALIN; OXYMETAZOLINE; PERTUSSIS TOXIN; RAUWOLSCINE; RECEPTOR SUBTYPE;

ANIMAL CELL; ARTICLE; CHO CELL; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG RECEPTOR BINDING; NONHUMAN; PRIORITY JOURNAL; SECOND MESSENGER; SIGNAL TRANSDUCTION;

ADRENERGIC ALPHA-AGONISTS; ANIMALS; CHO CELLS; CRICETINAE; GUANOSINE 5'-O-(3-THIOTRIPHOSPHATE); HUMANS; RECEPTORS, ADRENERGIC, ALPHA-2; SULFUR RADIOISOTOPES;

EID: 0031671118     PISSN: 00142999     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0014-2999(98)00518-4     Document Type: Article

References (16)

1. 1B receptor expression system. Mol. Pharmacol. 43, 427-433.
2. 2A-adrenergic receptor to multiple G-proteins. J. Biol. Chem. 269, 5730-5734.
3. 2-adrenergic receptors to two G-proteins with opposing effects. J. Biol. Chem. 267, 15795-15801.
4. 3H]RX821002 as radioligands. Biochim. Biophys. Acta 1226, 207-214.
5. Hilf, G., Jakobs, K.H., 1992. Agonist-independent inhibition of G protein activation by muscarinic acetylcholine receptor antagonists in cardiac membranes. Eur. J. Pharmacol. 225, 245-252.
6. 35S]GTPγS binding. Biochem. Pharmacol. 55, 1035-1043.
7. Lazareno, S., Farries, T., Birdsall, N.J.M., 1993. Pharmacological characterization of guanine nucleotide exchange reactions in membranes from CHO cells stably transfected with human muscarinic receptors m1-m4. Life Sci. 52, 449-456.
8. 35S]thio)triphosphate binding. Mol. Pharmacol. 44, 115-123.
9. 2A-adrenergic receptor subtype. Science 273, 801-803.
10. 3H]rauwolscine binding, inhibition of adenylate cyclase and RNase protection assay. Biochim. Biophys. Acta 1134, 169-177.
11. McKenzie, F.R., 1992. In: Milligan, G. (Ed.), Basic techniques to study G-protein function, in Signal transduction: a practical approach. Oxford Univ. Press, New York, pp. 31-56.
12. 2-Adrenoceptor regulation of adenylyl cyclase in CHO cells: dependence on receptor density, receptor subtype and present activity of adenylyl cyclase. Eur. J. Pharmacol. 335, 53-63.
13. Selley, D.E., Sim, L.J., Xiao, R., Liu, Q., Childers, S.R., 1997. μ-Opioid receptor-stimulated guanosine-5′-O-(γ-thio)-triphosphate binding in rat thalamus and cultured cell lines: signal transduction mechanisms underlying agonist efficacy. Mol. Pharmacol. 51, 87-96.
14. 35S]GTPγS binding. Eur. J. Pharmacol. 320, 267-275.
15. 2-adrenergic receptor activation of G proteins: evidence for a pre-coupled receptor/G protein state. Mol. Pharmacol. 45, 525-531.
16. z-mediated hormonal inhibition of cyclic AMP accumulation. Science 255, 339-342.