Production of human antibody repertoires in transgenic mice

Current Opinion in Biotechnology

Volumn 8, Issue 4, 1997, Pages 455-458

  Brüggemann, Marianne ^a   Taussig, Michael J ^a  

^a BABRAHAM INSTITUTE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

IMMUNOGLOBULIN HEAVY CHAIN; IMMUNOGLOBULIN LIGHT CHAIN; MONOCLONAL ANTIBODY;

ANIMAL EXPERIMENT; ANTIBODY AFFINITY; ANTIBODY PRODUCTION; ANTIBODY RESPONSE; ANTIGEN BINDING; B LYMPHOCYTE; GENE EXPRESSION; GENE MUTATION; GENE REARRANGEMENT; GENE TECHNOLOGY; IMMUNIZATION; LYMPHOID TISSUE; MOUSE; NONHUMAN; PRIORITY JOURNAL; SHORT SURVEY; SILENT GENE; TRANSGENE; TRANSGENIC MOUSE; YEAST ARTIFICIAL CHROMOSOME;

ANIMALIA; MUS MUSCULUS;

EID: 0342437532     PISSN: 09581669     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0958-1669(97)80068-7     Document Type: Article

References (24)

1. Brüggemann M, Neuberger MS: Strategies for expressing human antibody repertoires in transgenic mice. Immunol Today 1996, 17:391-397. A summary of the events and results in producing human antibody repertoires in transgenic mice with maps of the translocus regions.
2. Neuberger M, Brüggemann M: Mice perform a human repertoire. Nature 1997, 386:25-26. A comparison of two equally important strategies to produce human antibodies: transgenic mice, in vivo, and the phage display system, in vitro.
3. Zou X, Xian J, Davies NP, Popov AV, Brüggemann M: Dominant expression of a 1.3Mb human Igκ locus replacing mouse light chain production. FASEB J 1996, 10:1227-1232. This paper shows for the first time that complete megabase size regions can be transferred and dominantly expressed in transgenic mice. The expression of human Igκ transgenes can lead to a down regulation of mouse Igγ light chain expression.
4. κ segments, human antibody expression in endogenous knock-out mice closely resembles that seen in humans with respect to rearrangement, assembly and repertoire. A variety of specific human antibodies with high affinity have been produced.
5. Taylor LD, Carmack CE, Huszar D, Higgins KM, Mashayekh R, Sequar G, Schramm SR, Kuo C-C, O'Donnell SL, Kay RM et al.: Human immunoglobulin transgenes undergo rearrangement, somatic mutation and class switching in mice that lack endogenous IgM. Int Immunol 1993, 6:579-591.
6. Lonberg N, Taylor LD, Harding FA, Troustine M, Higgins KM, Schramm SR, Kuo C-C, Mashayekh R, Wymore K, McCabe JG et al.: Antigen-specific human antibodies from mice comprising four distinct genetic modifications. Nature 1994, 368:656-859.
7. Fishwild DM, O'Donnell SL, Bengoechea T, Hudson DV, Harding F, Bernhard SL, Jones D, Kay RM, Higgins KM, Schramm SR, Lonberg N: High-avidity human Igκ monoclonal antibodies from a novel strain of minilocus transgenic mice. Nat Biotechnol 1996, 14:845-851. Production of high affinity human anti-CD4 antibodies after class switching.
8. Popov AV, Bützler C, Frippiat J-P, Lefranc MP, Brüggemann M: Assembly and extension of yeast artificial chromosomes to build up a large locus. Gene 1996, 177:195-205. Of general interest for researchers who wish to construct or extend large regions on YACs. The method provides a positive/negative selection strategy to obtain authentic regions by site-specific integration.
9. Jakobovits A: Production of fully human antibodies by transgenic mice. Curr Opin Biotechnol 1995, 6:561-566.
10. Wagner SD, Popov AV, Davies SL, Xian J, Neuberger MS, Brüggemann M: The diversity of antigen-specific monoclonal antibodies from transgenic mice bearing human immunoglobulin gene mini loci. Eur J Immunol 1994, 24:2672-2681.
11. Knight KL, Kingzette M, Crane MA, Zhai S-K: Transchromosomally derived Ig heavy chains. J Immunol 1995, 155:684-691.
12. Green LL, Hardy MC, Maynard-Currie CE, Tsuda H, Louie DM, Mendez MJ, Abderrahim H, Noguchi M, Smith DH, Zeng Y, David et al.: Antigen-specific human monoclonal antibodies from mice engineered with human Ig heavy and light chain YACs. Nat Genet 1994, 7:13-21.
13. Wagner SD, Williams GT, Larson T, Neuberger MS, Kitamura D, Rajewsky K, Xian J, Brüggemann M: Antibodies generated from human immunoglobulin miniloci in transgenic mice. Nucleic Acids Res 1994, 22:1389-1393.
14. Tuaillon N, Miller AB, Tucker PW, Capra JD: Analysis of directed and inverted DJH rearrangements in a human Ig heavy chain transgenic minilocus. J Immunol 1995, 154:6453-6465.
15. H26 gene from naive transgenic mice display a wide range of antigen binding specificities. Immunology 1996, 88:174-182.
16. Wagner SD, Gross G, Cook GP, Davies SL, Neuberger MS: Antibody expression from the core region of the human IgH locus reconstructed in transgenic mice using bacteriophage P1 clones. Genomics 1996, 35:405-414.
17. Harding FA, Lonberg N: Class switching in human immunoglobulin transgenic mice. Ann NY Acad Sci 1995, 764:536-547.
18. Hofker MH, Walter MA, Cox DW: Complete physical map of the human immunoglobulin heavy chain constant region gene complex. Proc Natl Acad Sci USA 1989, 86:5567-5571.
19. H repertoire. Immunol Today 1995, 5:237-242.
20. Brüggemann M, Caskey HM, Teale C, Waldmann H, Williams GT, Surani MA, Neuberger MS: A repertoire of monoclonal antibodies with human heavy-chains from transgenic mice. Proc Natl Acad Sci USA 1989, 86:6709-6713.
21. Brüggemann M, Spicer C, Buluwela L, Rosewell I, Barton S, Surani MA, Rabbitts TH: Human antibody production in transgenic mice: expression from 100kb of the human IgH locus. Eur J Immunol 1991, 21:1323-1326.
22. Taylor LD, Carmack CE, Schramm SR, Mashayekh R, Higgins KM, Kuo C-C, Woodhouse C, Kay RM, Lonberg N: A transgenic mouse that expresses a diversity of human sequence heavy and light chain immunoglobulins. Nucleic Acids Res 1992, 20:6287-6295.
23. Choi TK, Hollenbach PW, Pearson BE, Ueda RM, Weddell GN, Kurahara CG, Woodhouse CS, Kay RM, Loring JF: Transgenic mice containing a human heavy chain immunoglobulin gene fragment cloned in a yeast artificial chromosome. Nat Genet 1993, 4:117-123.
24. Davies NP, Rosewell IR, Richardson JC, Cook GP, Neuberger MS, Brownstein BH, Norris ML, Brüggemann M: Creation of mice expressing human antibody light chains by introduction of a yeast artificial chromosome containing the core region of the human immunoglobulin K locus. Bio-Technology 1993, 11:911-914.