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1
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0343360846
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In vitro selected oligonucleotides as tools in organic chemistry
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Jäschke A., Frauendorf C., Hausch F. In vitro selected oligonucleotides as tools in organic chemistry. Synlett. 825-833:1999.
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(1999)
Synlett
, vol.825-833
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Jäschke, A.1
Frauendorf, C.2
Hausch, F.3
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4
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0033153344
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Boundaries for an RNA world
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Yarus M. Boundaries for an RNA world. Curr Opin Chem Biol. 3:1999;260-267.
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(1999)
Curr Opin Chem Biol
, vol.3
, pp. 260-267
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Yarus, M.1
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5
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0032087815
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Oligonucleotide libraries - variatio delectat
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Famulok M., Jenne A. Oligonucleotide libraries - variatio delectat. Curr Opin Chem Biol. 2:1998;320-327.
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(1998)
Curr Opin Chem Biol
, vol.2
, pp. 320-327
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Famulok, M.1
Jenne, A.2
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6
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0033102314
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A small catalytic RNA motif with Diels Alderase activity
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This is the first demonstration that a small unmodified RNA can catalyze C-C bond formation. Ribozymes were selected using a linker-coupled anthracene as the diene, and biotinylated maleimide as the dienophile. A 49-nucleotide minimal motif accelerated the reaction about 18,500-fold.
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Seelig B., Jäschke A. A small catalytic RNA motif with Diels Alderase activity. Chem Biol. 6:1999;167-176. This is the first demonstration that a small unmodified RNA can catalyze C-C bond formation. Ribozymes were selected using a linker-coupled anthracene as the diene, and biotinylated maleimide as the dienophile. A 49-nucleotide minimal motif accelerated the reaction about 18,500-fold.
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(1999)
Chem Biol
, vol.6
, pp. 167-176
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Seelig, B.1
Jäschke, A.2
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7
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0033594331
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Characteristics of an RNA Diels-Alderase active site
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Tarasow T.M., Tarasow S.L., Tu C., Kellogg E., Eaton B.E. Characteristics of an RNA Diels-Alderase active site. J Am Chem Soc. 121:1999;3614-3617.
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(1999)
J Am Chem Soc
, vol.121
, pp. 3614-3617
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Tarasow, T.M.1
Tarasow, S.L.2
Tu, C.3
Kellogg, E.4
Eaton, B.E.5
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8
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0033601113
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Cholesterol esterase activity by in vitro selection of RNA against a phosphate transition-state analogue
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This is one of the rare cases in which selection against a transition state analog actually gives a catalyst.
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Chun S-M., Jeong S., Kim J-M., Chong B-O., Park Y-K., Park H., Yu J. Cholesterol esterase activity by in vitro selection of RNA against a phosphate transition-state analogue. J Am Chem Soc. 121:1999;10844-10845. This is one of the rare cases in which selection against a transition state analog actually gives a catalyst.
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(1999)
J Am Chem Soc
, vol.121
, pp. 10844-10845
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Chun, S.-M.1
Jeong, S.2
Kim, J.-M.3
Chong, B.-O.4
Park, Y.-K.5
Park, H.6
Yu, J.7
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9
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0032706595
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A tiny RNA that catalyzes both aminoacyl-RNA and peptidyl-RNA synthesis
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This paper describes a 29-nucleotide RNA catalyzing successively the two related reactions, given phenylalanyl adenylate as substrate. In view of the RNA-world hypothesis, successive aminoacyl- and peptidyl-RNA synthesis by one RNA suggests that uncoded but RNA-catalyzed peptide synthesis would evolve before the synthesis of coded peptides.
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Illangasekare M., Yarus M. A tiny RNA that catalyzes both aminoacyl-RNA and peptidyl-RNA synthesis. RNA. 5:1999;1482-1489. This paper describes a 29-nucleotide RNA catalyzing successively the two related reactions, given phenylalanyl adenylate as substrate. In view of the RNA-world hypothesis, successive aminoacyl- and peptidyl-RNA synthesis by one RNA suggests that uncoded but RNA-catalyzed peptide synthesis would evolve before the synthesis of coded peptides.
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(1999)
RNA
, vol.5
, pp. 1482-1489
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Illangasekare, M.1
Yarus, M.2
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10
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0033545853
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Specific, rapid synthesis of Phe-RNA by RNA
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Illangasekare M., Yarus M. Specific, rapid synthesis of Phe-RNA by RNA. Proc Natl Acad Sci USA. 96:1999;5470-5475.
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(1999)
Proc Natl Acad Sci USA
, vol.96
, pp. 5470-5475
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Illangasekare, M.1
Yarus, M.2
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11
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0033229805
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A ribozyme and a catalytic DNA with peroxidase activity: Active sites versus cofactor-binding sites
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Travascio P., Bennet A.J., Wang D.Y., Sen D. A ribozyme and a catalytic DNA with peroxidase activity: active sites versus cofactor-binding sites. Chem Biol. 6:1999;779-787.
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(1999)
Chem Biol
, vol.6
, pp. 779-787
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Travascio, P.1
Bennet, A.J.2
Wang, D.Y.3
Sen, D.4
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12
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0028124128
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In vitro evolution of new ribozymes with polynucleotide kinase activity
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Lorsch J.R., Szostak J.W. In vitro evolution of new ribozymes with polynucleotide kinase activity. Nature. 371:1994;31-36.
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(1994)
Nature
, vol.371
, pp. 31-36
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Lorsch, J.R.1
Szostak, J.W.2
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13
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0033019927
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Phosphorylating DNA with DNA
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An illustrative example of the use of in vitro evolution and counter selection to fine-tune the molecular properties of nucleic-acid catalysts. Each of the selected deoxyribozymes makes use of one or more of the eight-standard NTPs or dNTPs as a source of activated phosphate. One optimized deoxyribozyme uses ATP more than 40,000-fold more efficiently than the other ribonucleoside triphosphates.
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Li Y., Breaker R.R. Phosphorylating DNA with DNA. Proc Natl Acad Sci USA. 96:1999;2746-2751. An illustrative example of the use of in vitro evolution and counter selection to fine-tune the molecular properties of nucleic-acid catalysts. Each of the selected deoxyribozymes makes use of one or more of the eight-standard NTPs or dNTPs as a source of activated phosphate. One optimized deoxyribozyme uses ATP more than 40,000-fold more efficiently than the other ribonucleoside triphosphates.
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(1999)
Proc Natl Acad Sci USA
, vol.96
, pp. 2746-2751
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Li, Y.1
Breaker, R.R.2
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14
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0033581938
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A ribozyme that lacks cytidine
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Starting from an RNA molecule that contained roughly equal proportions of all four nucleotides, in vitro evolution yielded an RNA ligase ribozyme that completely lacks cytidine.
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Rogers J., Joyce G.F. A ribozyme that lacks cytidine. Nature. 402:1999;323-325. Starting from an RNA molecule that contained roughly equal proportions of all four nucleotides, in vitro evolution yielded an RNA ligase ribozyme that completely lacks cytidine.
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(1999)
Nature
, vol.402
, pp. 323-325
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Rogers, J.1
Joyce, G.F.2
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15
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0033485897
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A molecular description of the evolution of resistance
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Ordoukhanian P., Joyce G.F. A molecular description of the evolution of resistance. Chem Biol. 6:1999;881-889.
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(1999)
Chem Biol
, vol.6
, pp. 881-889
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Ordoukhanian, P.1
Joyce, G.F.2
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16
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0033524391
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Emergence of a dual-catalytic RNA with metal-specific cleavage and ligase activities: The spandrels of RNA evolution
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Landweber L.F., Pokrovskaya I.D. Emergence of a dual-catalytic RNA with metal-specific cleavage and ligase activities: the spandrels of RNA evolution. Proc Natl Acad Sci USA. 96:1999;173-178.
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(1999)
Proc Natl Acad Sci USA
, vol.96
, pp. 173-178
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Landweber, L.F.1
Pokrovskaya, I.D.2
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17
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0032955410
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In vitro selection of an allosteric ribozyme that transduces analytes to amplicons
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An allosteric ribozyme was selected that is activated by oligonucleotide effectors and conforms to a classic two-state model for allostery.
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Robertson M.P., Ellington A.D. In vitro selection of an allosteric ribozyme that transduces analytes to amplicons. Nat Biotechnol. 17:1999;62-66. An allosteric ribozyme was selected that is activated by oligonucleotide effectors and conforms to a classic two-state model for allostery.
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(1999)
Nat Biotechnol
, vol.17
, pp. 62-66
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Robertson, M.P.1
Ellington, A.D.2
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18
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0033616774
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Engineering precision RNA molecular switches
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Ligand-specific molecular switches composed of RNA were created by coupling pre-existing catalytic and receptor domains by structural bridges. Small organic molecules can switch catalytic activity on or off.
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Soukup G.A., Breaker R.R. Engineering precision RNA molecular switches. Proc Natl Acad Sci USA. 96:1999;3584-3589. Ligand-specific molecular switches composed of RNA were created by coupling pre-existing catalytic and receptor domains by structural bridges. Small organic molecules can switch catalytic activity on or off.
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(1999)
Proc Natl Acad Sci USA
, vol.96
, pp. 3584-3589
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Soukup, G.A.1
Breaker, R.R.2
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19
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0032755647
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Allosteric selection of ribozymes that respond to the second messengers cGMP and cAMP
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In vitro selected ribozymes are specifically activated by cyclic nucleotide effectors by a factor of 5000, which is the largest allosteric activation of a ribozyme by a small organic molecule reported to date.
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Koizumi M., Soukup G.A., Kerr J.N., Breaker R.R. Allosteric selection of ribozymes that respond to the second messengers cGMP and cAMP. Nat Struct Biol. 6:1999;1062-1071. In vitro selected ribozymes are specifically activated by cyclic nucleotide effectors by a factor of 5000, which is the largest allosteric activation of a ribozyme by a small organic molecule reported to date.
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(1999)
Nat Struct Biol
, vol.6
, pp. 1062-1071
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Koizumi, M.1
Soukup, G.A.2
Kerr, J.N.3
Breaker, R.R.4
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21
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0031151974
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The joys of in vitro selection: Chemically dressing oligonucleotides to satiate protein targets
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Eaton B.E. The joys of in vitro selection: chemically dressing oligonucleotides to satiate protein targets. Curr Opin Chem Biol. 1:1997;10-16.
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(1997)
Curr Opin Chem Biol
, vol.1
, pp. 10-16
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Eaton, B.E.1
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22
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0032426662
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The antibody catalysis route to the total synthesis of epothilones
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Sinha S.C., Barbas C.F. III, Lerner R.A. The antibody catalysis route to the total synthesis of epothilones. Proc Natl Acad Sci USA. 95:1998;14603-14608.
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(1998)
Proc Natl Acad Sci USA
, vol.95
, pp. 14603-14608
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Sinha, S.C.1
Barbas C.F. III2
Lerner, R.A.3
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23
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0031854986
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Man-made cell-like compartments for molecular evolution
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Tawfik D.S., Griffiths A.D. Man-made cell-like compartments for molecular evolution. Nat Biotechnol. 16:1998;652-656.
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(1998)
Nat Biotechnol
, vol.16
, pp. 652-656
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Tawfik, D.S.1
Griffiths, A.D.2
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