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Volumn 121, Issue 46, 1999, Pages 10844-10845

Cholesterol esterase activity by in vitro selection of RNA against a phosphate transition-state analogue [9]

Author keywords

[No Author keywords available]

Indexed keywords

CHOLESTEROL ESTERASE; PHOSPHATE; RNA;

EID: 0033601113     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja991848u     Document Type: Letter
Times cited : (23)

References (41)
  • 12
    • 0003604405 scopus 로고
    • Cold Spring Harbor Laboratory Press: Cold Spring Harbor
    • Joyce, G. F.; Orgel, L. E. The RNA World; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, 1989; p 1.
    • (1989) The RNA World , pp. 1
    • Joyce, G.F.1    Orgel, L.E.2
  • 13
    • 0024476917 scopus 로고
    • (b) Joyce, G. F. Nature 1989. 338, 217.
    • (1989) Nature , vol.338 , pp. 217
    • Joyce, G.F.1
  • 23
    • 13044251000 scopus 로고    scopus 로고
    • unpublished results
    • Schultz, P. G., unpublished results.
    • Schultz, P.G.1
  • 24
    • 13044282237 scopus 로고    scopus 로고
    • See Supporting Information
    • See Supporting Information.
  • 26
    • 13044289838 scopus 로고    scopus 로고
    • note
    • 13 of diversity. The DNA template was as shown below: 5′-CCATAATACGACTCACTATAGGGGAGCTCGGTACCGAATTC-N70-AAGCTTTGCAGAGGATCCTT- 3′ T7 RNA polymerase SacI KpnI EcoRI HindIII BamHI
  • 33
    • 13044265018 scopus 로고    scopus 로고
    • note
    • The random region of the sequence of this clone is as follows: 5′-GTGGGGTCGT CTTGGTTAAA CTCCTTGCGC GTCACGAGGT TAGC-CAGCTT GATACCTCAA GGTGGTGCCT-3′.
  • 37
    • 13044293171 scopus 로고    scopus 로고
    • note
    • 3 at pH 7.5). The product conversion was measured by injecting 10 μL aliquots of the reaction mixture onto an HPLC column and monitoring at 315 nm for p-nitrophenol. A C18 column (Microsorb; 4.6 mm × 15 cm, 5 μm) was used as the stationary phase and 50% aqueous acetonitrile as the mobile phase.
  • 38
    • 13044296833 scopus 로고    scopus 로고
    • note
    • To a 90 μL of RNA solution (0.67 μA) in assay buffer, 10 μL of 10 × substrate solution (variable from 0.15 mM to 1.0 mM in THF) was added. The resulting solution (0.6 μM of RNA, 15-100 μM of variable substrate concentration) was incubated at 37°C for 3 days. The product conversion was measured by injecting 10 μL aliquots of the reaction mixture onto an HPLC column in every 24 h.
  • 39
    • 13044286585 scopus 로고    scopus 로고
    • note
    • In the absence and presence of original pool RNA (0.6 μM), background hydrolysis rate of the substrate was measured, affording the same number.
  • 41
    • 13044291832 scopus 로고    scopus 로고
    • note
    • cat/RT)


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.