Increased skeletal muscle expression of PKC-θ but not PKC-α mRNA in type 2 diabetes: Inverse relationship with in-vivo insulin sensitivity

European Journal of Clinical Investigation

Volumn 33, Issue 11, 2003, Pages 983-987

  Gray, S ^a,b   Idris, I ^a,b   Davis, K R ^a,b   Donnelly, R ^a,b,c  

^a UNIVERSITY OF NOTTINGHAM   (United Kingdom)

^b UNIVERSITY OF NOTTINGHAM   (United Kingdom)

^c DERBYSHIRE ROYAL INFIRMARY   (United Kingdom)

Author keywords

Insulin resistance; Lipids, PKC ; PKC, type 2 diabetes

Indexed keywords

GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; INSULIN; MESSENGER RNA; PROTEIN KINASE C; PROTEIN KINASE C ALPHA; PROTEIN KINASE C THETA; UNCLASSIFIED DRUG;

ADULT; AGED; ARTICLE; BODY MASS; CLINICAL ARTICLE; CONTROLLED STUDY; DIABETIC DIET; GLUCOSE CLAMP TECHNIQUE; HUMAN; HUMAN TISSUE; HYPERINSULINEMIA; HYPERTRIGLYCERIDEMIA; INSULIN BLOOD LEVEL; INSULIN RESISTANCE; INSULIN SENSITIVITY; MALE; MUSCLE BIOPSY; NON INSULIN DEPENDENT DIABETES MELLITUS; ORAL GLUCOSE TOLERANCE TEST; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SKELETAL MUSCLE;

ADULT; AGED; CASE-CONTROL STUDIES; DIABETES MELLITUS, TYPE 2; HUMANS; INSULIN RESISTANCE; ISOENZYMES; MALE; MIDDLE AGED; MUSCLE, SKELETAL; PROTEIN KINASE C; PROTEIN KINASE C-ALPHA; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; UP-REGULATION;

EID: 0242721945     PISSN: 00142972     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2362.2003.01246.x     Document Type: Article

References (10)

1. Itani SI, Zhou Q, Pories WJ, MacDonald KG, Dohm GL. Involvement of protein kinase C in human skeletal muscle insulin resistance and obesity. Diabetes 2000;49: 1353-8.
2. Donnelly R, Reed MJ, Azhar S, Reaven GM. Expression of the major isoenzyme of protein kinase C in skeletal muscle, PKC-θ, varies with muscle type and in response to fructose-induced insulin resistance. Endocrinology 1994;135:2369-74.
3. Schmitz-Peiffer C, Browne CL, Oakes ND, Watkinson A, Chisholm DJ, Kraegen EW et al. Alterations in the expression and cellular localisation of protein kinase C isozymes ε and θ are associated with insulin resistance in skeletal muscle of the high-fat fed rat. Diabetes 1997;46:169-78.
4. Idris I, Gray S, Donnelly R. Protein kinase C activation: isozyme-specific effects on metabolism and cardiovascular complications in diabetes. Diabetologia 2001;44:659-73.
5. Strack V, Hennige AM, Krutzfeldt J et al. Serine residues 994 and 1023/ 25 are important for insulin receptor kinase inhibition by protein kinase C isoforms β2 and θ. Diabetologia 2000;43:443-9.
6. Griffin ME, Marcucci MJ, Cline GW et al. Free fatty acid induced insulin resistance is associated with activation of protein kinase C-θ and alterations in the insulin signalling cascade. Diabetes 1999;48:1270-4.
7. Kellerer M, Muschak J, Seffer E, Mischak H, Ullrich A, Haring HU. Protein kinase C isoforms α, δ and θ require insulin receptor substrate-1 to inhibit the tyrosine kinase activity of the insulin receptor in human kidney embryonic cells (HEK 293 cells). Diabetologia 1998;41:833-8.
8. Itani SI, Pories WJ, Macdonald KG, Dohm GL. Increased protein kinase C-θ in skeletal muscle of diabetic patients. Metabolism 2001;50:553-7.
9. Kofler K, Kochl S, Parson W, Erdel M, Utermann G, Baier G. Molecular characterization of the human protein kinase C-theta gene locus. Mol General Genet 1998;259:398-403.
10. Avignon A, Standaert ML, Yamada K, Mischak H, Spencer B, Farese RV. Insulin increases mRNA levels of protein kinase C-α and -β in rat adipocytes and PKC-α, -β and -θ in rat skeletal muscle. Biochem J 1995;308:181-7.