Comparison of assay technologies for a nuclear receptor assay screen reveals differences in the sets of identified functional antagonists

Journal of Biomolecular Screening

Volumn 8, Issue 4, 2003, Pages 381-392

  Wu, Xiang ^a   Glickman, J Fraser ^b   Bowen, Benjamin R ^b   Sills, Matthew A ^c  

^a EXELIXIS INC   (United States)

^b NOVARTIS INSTITUTES FOR BIOMEDICAL RESEARCH   (United States)

^c PHARMACOPEIA INC   (United States)

Author keywords

AlphaScreen; High throughput screening; Nuclear receptor assay screens; Time resolved fluorescence (TRF); Time resolved fluorescence resonance energy transfer (TR FRET)

Indexed keywords

CELL NUCLEUS RECEPTOR; RECEPTOR BLOCKING AGENT; CELL RECEPTOR; DNA BINDING PROTEIN; FARNESOID X RECEPTOR; FARNESOID X-ACTIVATED RECEPTOR; HYBRID PROTEIN; LIGAND; TRANSCRIPTION FACTOR;

ARTICLE; CONTROLLED STUDY; DOSE RESPONSE; DRUG ACTIVITY; FLUORESCENCE ANALYSIS; FLUORESCENCE RESONANCE ENERGY TRANSFER; HIGH THROUGHPUT SCREENING; INTERMETHOD COMPARISON; LABORATORY; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; TECHNOLOGY; ANIMAL; BIOCHEMISTRY; COMPARATIVE STUDY; DRUG ANTAGONISM; DRUG SCREENING; EVALUATION; METABOLISM; METHODOLOGY; PROTEIN BINDING; RAT; SPECTROFLUOROMETRY;

ANIMALS; BIOCHEMISTRY; DNA-BINDING PROTEINS; DOSE-RESPONSE RELATIONSHIP, DRUG; DRUG EVALUATION, PRECLINICAL; FLUORESCENCE RESONANCE ENERGY TRANSFER; LIGANDS; PROTEIN BINDING; RATS; RECEPTORS, CYTOPLASMIC AND NUCLEAR; RECOMBINANT FUSION PROTEINS; SPECTROMETRY, FLUORESCENCE; TRANSCRIPTION FACTORS;

EID: 0242483911     PISSN: 10870571     EISSN: None     Source Type: Journal    
DOI: 10.1177/1087057103256466     Document Type: Article

References (7)

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