Enforced adenoviral vector-mediated expression of HOXB4 in human umbilical cord blood CD34+ cells promotes myeloid differentiation but not proliferation

Molecular Therapy

Volumn 8, Issue 4, 2003, Pages 618-628

  Brun, Ann C M ^a   Fan, Xiaolong ^a   Björnsson, Jon Mar ^a   Humphries, R Keith ^b   Karlsson, Stefan ^a  

^a LUND UNIVERSITY HOSPITAL   (Sweden)

^b BRITISH COLUMBIA CANCER AGENCY   (Canada)

Author keywords

Adenovirus; Gene therapy; HOXB4; Human; Jematopoietic stem cells

Indexed keywords

ADENOVIRUS VECTOR; CD34 ANTIGEN; GREEN FLUORESCENT PROTEIN; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR HOXB4; UNCLASSIFIED DRUG;

ARTICLE; BONE MARROW CELL; CELL CULTURE; CELL DIFFERENTIATION; CELL PROLIFERATION; CELL SELECTION; CELL VIABILITY; CONTROLLED STUDY; GENE OVEREXPRESSION; GENE SEQUENCE; GENETIC TRANSDUCTION; GENETIC TRANSFECTION; HEMATOPOIESIS; HEMATOPOIETIC STEM CELL; HUMAN; HUMAN CELL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TRANSGENE; UMBILICAL CORD BLOOD; VIRAL GENE DELIVERY SYSTEM; WESTERN BLOTTING;

ADENOVIRIDAE; MURINAE;

EID: 0142181988     PISSN: 15250016     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1525-0016(03)00237-5     Document Type: Article

References (34)

1. Orkin, S. H. (2000). Diversification of haematopoietic stem cells to specific lineages. Nat. Rev. Genet. 1: 57-64.
2. Chauvet, S., Merabet, S., Bilder, D., Scott, M. P., Pradel, J., and Graba, Y. (2000). Distinct hox protein sequences determine specificity in different tissues. Proc. Natl. Acad. Sci. USA 97: 4064-4069.
3. Gibson, G. (2000). Evolution: hox genes and the cellared wine principle. Curr. Biol. 10: R452-455.
4. Lawrence, H. J., Sauvageau, G., Humphries, R. K., and Largman, C. (1996). The role of HOX homeobox genes in normal and leukemic hematopoiesis. Stem Cells 14: 281-291.
5. Giampaolo, A., et al. (1995). HOXB gene expression and function in differentiating purified hematopoietic progenitors. Stem Cells 13(Suppl. 1): 90-105.
6. Antonchuk, J., Sauvageau, G., and Humphries, R. K. (2001). HOXB4 overexpression mediates very rapid stem cell regeneration and competitive hematopoietic repopulation. Exp. Hematol. 29: 1125-1134.
7. Buske, C., and Humphries, R. K. (2000): Homeobox genes in leukemogenesis. Int. J. Hematol. 71: 301-308.
8. Pineault, N., Helgason, C. D., Lawrence, H. J., and Humphries, R. K. (2002). Differential expression of Hox, Meis1, and Pbx1 genes in primitive cells throughout murine hematopoietic ontogeny. Exp. Hematol. 30: 49-57.
9. Thorsteinsdottir, U., Sauvageau, G., and Humphries, R. K. (1999). Enhanced in vivo regenerative potential of HOXB4-transduced hematopoietic stem cells with regulation of their pool size. Blood 94: 2605-2612.
10. Antonchuk, J., Sauvageau, G., and Humphries, R. K. (2002). HOXB4-induced expansion of adult hematopoietic stem cells ex vivo. Cell 109: 39-45.
11. Björnsson, J. M., Brun, A., Larsson, N., Repetowska, E., and Karlsson, S. (2001). HoxB4 deficiency causes reduced cellularity in hematopoietic organs and diminished proliferative response of primitive hematopoietic progenitors. Blood 98: 69a [Abstract].
12. Björnsson J. M. et al. 2003 Reduced proliferative capacity of hematopoietic stem cells deficient in Hoxb3 and Hoxb4. Mol. Cell. Biol. 23: 3872-3883.
13. Buske, C., et al. (2002). Deregulated expression of HOXB4 enhances the primitive growth activity of human hematopoietic cells. Blood 100: 862-868.
14. + cells, but impairs lymphomyeloid differentiation. Blood 101: 1759-1768.
15. Fan, X., et al. (2002). Transient disruption of autocrine TGF-beta signaling leads to enhanced survival and proliferation potential in single primitive human hemopoietic progenitor cells. J. Immunol. 168: 755-762.
16. Fan, X., Brun, A., Segren, S., Jacobsen, S. E., and Karlsson, S. (2000). Efficient adenoviral vector transduction of human hematopoietic SCID-repopulating and long-term culture-initiating cells. Hum. Gene Ther. 11: 1313-1327.
17. Fan, X., Brun, A., and Karlsson, S. (2000). Adenoviral vector design for high-level transgene expression in primitive human hematopoietic progenitors. Gene Ther. 7: 2132-2138.
18. Hooiveld, M. H., et al. (1999). Novel interactions between vertebrate Hox genes. Int. J. Dev. Biol. 43: 665-674.
19. Care, A., et al. (1994). Coordinate expression and proliferative role of HOXB genes in activated adult T lymphocytes. Mol. Cell. Biol. 14: 4872-4877.
20. Sauvageau, G., et al. (1995). Overexpression of HOXB4 in hematopoietic cells causes the selective expansion of more primitive populations in vitro and in vivo. Genes Dev. 9: 1753-1765.
21. Feuring-Buske, M., Buske, C., Hogge, D., Eaves, C., Coulombel, L., and Humphries, R. K. (2000). Overexpression of HoxB4 enhances the expansion of primitive human hematopoietic progenitor cells and promotes erythroid differentiation in vitro and in vivo. Blood 96: 536a [Abstract].
22. Borge, O. J., Ramsfjell, V., Veiby, O. P., Murphy, M. J., Lok, S., and Jacobsen, S. E. (1996). Thrombopoietin, but not erythropoietin promotes viability and inhibits apoptosis of multipotent murine hematopoietic progenitor cells in vitro. Blood 88: 2859-2870.
23. Olivieri, N. F., et al. (1991 ). Diamond-Blackfan anemia: heterogenous response of hematopoietic progenitor cells in vitro to the protein product of the steel locus. Blood 78: 2211-2215.
24. Nerlov, C., and Graf, T. (1998). PU.1 induces myeloid lineage commitment in multipotent hematopoietic progenitors. Genes Dev. 12: 2403-2412.
25. Kulessa, H., Frampton, J., and Graf, T. (1995). GATA-1 reprograms avian myelomonocytic cell lines into eosinophils, thromboblasts, and erythroblasts. Genes Dev. 9: 1250-1262.
26. Pan, Q., and Simpson, R. U. (1999). c-myc intron element-binding proteins are required for 1, 25-dihydroxyvitamin D3 regulation of c-myc during HL-60 cell differentiation and the involvement of HOXB4. J. Biol. Chem. 274: 8437-8444.
27. Pan, Q., and Simpson, R. U. (2001). Antisense knockout of HOXB4 blocks 1,25-dihydroxyvitamin D3 inhibition of c-myc expression. J. Endocrinol. 169: 153-159.
28. Krosl, J., Beslu, N., Mayotte, N., Humphries, R. K., and Sauvageau, G. (2003). The competitive nature of HOXB4-transduced HSC is limited by PBX1: the generation of ultra-competitive stem cells retaining full differentiation potential. Immunity 18: 561-571.
29. Neering, S. J., Hardy, S. F., Minamoto, D., Spratt, S. K., and Jordan, C. T. (1996). Transduction of primitive human hematopoietic cells with recombinant adenovinus vectors. Blood 88: 1147-1155.
30. He, T. C., Zhou, S., da Costa, L. T., Yu, J., Kinzler, K. W., and Vogelstein, B. (1998). A simplified system for generating recombinant adenoviruses. Proc. Natl. Acad. Sci. USA 95: 2509-2514.
31. Hitt (1995). Techniques for human adenovirus construction and characterization. Methods Mol. Genet. 7: 13-30.
32. Howard, D. S., et al. (1999). Genetic manipulation of primitive leukemic and normal hematopoietic cells using a novel method of adenovirus-mediated gene transfer. Leukemia 13: 1608-1616.
33. Hogge, D. E., Lansdorp, P. M., Reid, D., Gerhard, B., and Eaves, C. J. (1996). Enhanced detection, maintenance, and differentiation of primitive human hematopoietic cells in cultures containing murine fibroblasts engineered to produce human steel factor, interleukin-3, and granulocyte colony-stimulating factor. Blood 88: 3765-3773.
34. Buske, C., et al. (2001). Overexpression of HOXA10 perturbs human lymphomyelopoiesis in vitro and in vivo. Blood 97: 2286-2292.