Quantitative assessment of cell viability and apoptosis in cultured epidermal autografts: Application to burn therapy

International Journal of Artificial Organs

Volumn 26, Issue 9, 2003, Pages 793-803

  Vernez, M ^a   Raffoul, W ^a   Gailloud Matthieu, M C ^a   Egloff, D ^a   Senechaud, I ^a   Panizzon, R G ^a   Benathan, Messod ^a  

^a LAUSANNE UNIVERSITY HOSPITAL   (Switzerland)

Author keywords

Apoptosis; Burns; Cultured epidermal autografts; Keratinocytes; Skin transplantation

Indexed keywords

PATIENT TREATMENT;

BIOLOGICAL PROPERTIES; BURN; CELL APOPTOSIS; CELL VIABILITY; CULTURED EPIDERMAL AUTOGRAFT; DNA FRAGMENT; END-LABELING; KERATINOCYTES; QUANTITATIVE ASSESSMENTS; SKIN TRANSPLANTATION;

CELL DEATH;

BIOLOGICAL FACTOR; CASPASE 3; DNA FRAGMENT; GENTAMICIN; PROTEIN GLUTAMINE GAMMA GLUTAMYLTRANSFERASE;

ADOLESCENT; ADULT; APOPTOSIS; ARTICLE; BIOASSAY; BURN; CELL VIABILITY; CHILD; CLINICAL ARTICLE; CLINICAL TRIAL; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; DNA DETERMINATION; ENZYME ACTIVITY; FEMALE; HOSPITAL ADMISSION; HUMAN; MALE; NICK END LABELING; QUANTITATIVE ANALYSIS; SKIN AUTOGRAFT; SKIN CULTURE; SKIN TRANSPLANTATION; SWITZERLAND; UNIVERSITY HOSPITAL;

EID: 0142167948     PISSN: 03913988     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (48)

1. Green H. Cyclic AMP in relation to proliferation of the epidermal cell: A new view. Cell 1978; 15: 801-11.
2. Rheinwald JG, Green H. Epidermal growth factor and the multiplication of cultured human epidermal keratinocytes. Nature 1977; 265: 421-4.
3. Green H, Kehinde O, Thomas J. Growth of cultured human epidermal cells into multiple epithelia suitable for grafting. Proc Natl Acad Sci USA 1979; 76: 5665-8.
4. Walzer C, Benathan M, Frenk E. Thermolysin treatment: A new method for dermo-epidermal separation. J Invest Dermatol 1989; 92: 78-81.
5. Gallico GG, O'Connor NE, Compton CC, Kehinde O, Green H. Permanent coverage of large burn wounds with autologous cultured human epithelium. N Engl J Med 1984; 311: 448-51.
6. Meuli M, Raghunath M. Burns (Part 2). Tops and flops using cultured epithelial autografts in children. Pediatr Surg Int 1997; 12: 471-7.
7. Odessey R. Addendum: Multicenter experience with cultured epidermal autograft for treatment of burns. J Burn Care Rehabil 1992; 13: 174-80.
8. Gobet R, Raghunath M, Altermatt S, Meuli-Simmen C, Benathan M, Dieti A, et al. Efficacy of cultured epithelial autografts in pediatric burns and reconstructive surgery. Surgery 1997; 121: 654-61.
9. Paddie-Ledinek JE, Cruickshank DG, Masterton JP. Skin replacement by cultured keratinocyte grafts: An Australian experience. Burns 1997; 23: 204-11.
10. Carsin H, Ainaud P, Le Bever H, Rives J, Lakhel A, Stephanazzi J, et al. Cultured epithelial autografts in extensive burn coverage of severely traumatized patients: A five year single-center experience with 30 patients. Burns 2000; 26: 379-87.
11. Desai MH, Miakar JM, McCauley RL, Abdullah KM, Rutan RL, Waymack JP, et al. Lack of long-term durability of cultured keratinocyte burn-wound coverage: A case report. J Burn Care Rehabil 1991; 12: 540-5.
12. Clugston PA, Snelling CF, Macdonald IB, Maledy HL, Boyle JC, Germann E, et al. Cultured epithelial autografts: Three years of clinical experience with eighteen patients. J Burn Care Rehabil 1991; 12: 533-9.
13. Elliott M, Vandervord J. Initial experience with cultured epithelial autografts in massively burnt patients. ANZ J Surg 2002; 72: 893-5.
14. Cuono C, Langdon R, McGuire J. Use of cultured epidermal autografts and dermal allografts as skin replacement after burn injury. Lancet 1986; 1: 1123-4.
15. Krupp S, Benathan M, Meuli M, Deglise B, Hoizer E, Wiesner L, et al. Current concepts in pediatric burn care: Management of burn wounds with cultured epidermal autografts. Eur J Pediatr Surg 1992; 2: 210-5.
16. Compton CC, Hickerson W, Nadire K, Press W. Acceleration of skin regeneration from cultured epithelial autografts by transplantation to homograft dermis. J Burn Care Rehabil 1993; 14: 653-62.
17. Braye F, Oddou L, Bertin-Maghit M, Belgacem S, Damour O, Spitalier P, et al. Widely meshed autograft associated with cultured autologous epithelium for the treatment of major burns in children: Report of 12 cases. Eur J Pediatr Surg 2000; 10: 35-40.
18. Boyce ST. Skin substitutes from cultured cells and collagen-GAG polymers. Med Biol Eng Comput 1998; 36: 791-800.
19. Ronfard V, Rives JM, Neveux Y, Carsin H, Barrandon Y. Long-term regeneration of human epidermis on third degree burns transplanted with autologous cultured epithelium grown on a fibrin matrix. Transplantation 2000; 70:1588-98.
20. Streit M, Braathen LR. Apligraf - A living human skin equivalent for the treatment of chronic wounds. Int J Artif Organs 2000; 23: 831-3.
21. Beele H. Artificial skin: Past, present and future. Int J Artif Organs 2002;25:163-73.
22. Rheinwald JG, Green H. Serial cultivation of strains of human epidermal keratinocytes: The formation of keratinizing colonies from single cells. Cell 1975; 6: 331-43.
23. Damour O, Hua SZ, Lasne F, Villain M, Rousselle P, Collombel C. Cytotoxicity evaluation of antiseptics and antibiotics on cultured human fibroblasts and keratinocytes. Burns 1992; 18: 479-85.
24. Nicholson DW, Ali A, Thomberry NA, Vaillancourt JP, Ding CK, Gallant M, et al. Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis. Nature 1995; 376: 37-43.
25. Hohl D, Aeschlimann D, Huber M. In vitro and rapid in situ transglutaminase assays for congenital ichthyoses - A comparative study. J Invest Dermatol 1998; 110: 268-71.
26. Sedlak J, Lindsay RH. Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Anal Biochem 1968; 25:192-205.
27. Candi E, Oddi S, Terrinoni A, Paradisi A, Ranalli M, Finazzi-Agro A, et al. Transglutaminase 5 cross-links loricrin, involucrin, and small proline-rich proteins in vitro. J Biol Chem 2001; 276: 35014-35023.
28. Lin XR, Wilkinson DI, Farber EM. Camptothecin induces differentiation, tissue transglutaminase and apoptosis in cultured keratinocytes. Exp Dermatol 1998; 7:179-83.
29. Thornberry NA, Lazebnik Y. Caspases: Enemies within. Science 1998; 281:1312-6.
30. Maruoka Y, Harada H, Mitsuyasu T, Seta Y, Kurokawa H, Kajiyama M, et al. Keratinocytes become terminally differentiated in a process involving programmed cell death. Biochem Biophys Res Commun 1997; 238: 886-90.
31. Rodeck U, Jost M, DuHadaway J, Kari C, Jensen PJ, Risse B, et al. Regulation of Bcl-xL expression in human keratinocytes by cell-substratum adhesion and the epidermal growth factor receptor. Proc Natl Acad Sci USA 1997; 94: 5067-72.
32. Antonsson B, Martinou JC. The Bcl-2 protein family. Exp Cell Res 2000; 256: 50-7.
33. Takahashi H, Nakamura S, Asano K, Kinouchi M, Ishida-Yamamoto A, Iizuka H. Fas antigen modulates ultraviolet B-induced apoptosis of SVHK cells: Sequential activation of caspases 8, 3, and 1 in the apoptotic process. Exp Cell Res 1999; 249: 291-8.
34. Durante P, Gueuning MA, Darville MI, Hue L, Rousseau GG. Apoptosis induced by growth factor withdrawal in fibroblasts overproducing fructose 2,6-bisphosphate. FEBS Lett 1999; 448: 239-43.
35. Somervaille TC, Linch DC, Khwaja A. Growth factor withdrawal from primary human erythroid progenitors induces apoptosis through a pathway involving glycogen synthase kinase-3 and Bax. Blood 2001; 98: 1374-81.
36. Gniadecki R, Jemec GB, Thomsen BM, Hansen M. Relationship between keratinocyte adhesion and death: Anoikis in acantholytic diseases. Arch Dermatol Res 1998; 290: 528-32.
37. Nuzzo I, Sanges MR, Folgore A, Carratelli CR. Apoptosis of human keratinocytes after bacterial invasion. FEMS Immunol Med Microbiol 2000; 27: 235-40.
38. Boyce ST, Kagan RJ, Meyer NA, Yakuboff KP, Warden GD. Cultured skin substitutes combined with Integra Artificial Skin to replace native skin autograft and allograft for the closure of excised full-thickness burns. J Burn Care Rehabil 1999; 20: 453-61.
39. Rossi A, Catani MV, Candi E, Bernassola F, Puddu P, Melino G. Nitric oxide inhibits cornified envelope formation in human keratinocytes by inactivating transglutaminases and activating protein 1. J Invest Dermatol 2000; 115: 731-9.
40. Meister A. Glutathione-ascorbic acid antioxidant system in animals. J Biol Chem 1994; 269: 9397-400.
41. Schnelldorfer T, Gansauge S, Gansauge F, Schlosser S, Beger HG, Nussler AK. Glutathione depletion causes cell growth inhibition and enhanced apoptosis in pancreatic cancer cells. Cancer 2000; 89: 1440-7.
42. Aoshiba K, Yasui S, Nishimura K, Nagai A. Thiol depletion induces apoptosis in cultured lung fibroblasts. Am J Respir Cell Mol Biol 1999; 21: 54-64.
43. Barrandon Y, Green H. Cell migration is essential for sustained growth of keratinocyte colonies: The roles of transforming growth factor-alpha and epidermal growth factor. Cell 1987; 50: 1131-7.
44. Pellegrini G, Ranno R, Stracuzzi G, Bondanza S, Guerra L, Zambruno G, et al. The control of epidermal stem cells (holoclones) in the treatment of massive full-thickness burns with autologous keratinocytes cultured on fibrin. Transplantation 1999; 68: 868-79.
45. Goretsky MJ, Harriger MD, Supp AP, Greenhalgh DG, Boyce ST. Expression of interleukin-1 alpha, interleukin-6, and basic fibroblast growth factor by cultured skin substitutes before and after grafting to full-thickness wounds in athymic mice. J Trauma 1996; 40: 894-9.
46. Ansel JC, Tiesman JP, Olerud JE, Krueger JG, Krane JF, Tara DC, et al. Human keratinocytes are a major source of cutaneous platelet-derived growth factor. J Clin Invest 1993; 92: 671-8.
47. Eming SA, Medalie DA, Tompkins RG, Yarmush ML, Morgan JR. Genetically modified human keratinocytes overexpressing PDGF-A enhance the performance of a composite skin graft. Hum Gene Ther 1998; 9: 529-39.
48. Somers T, Duinslaeger L, Delaey B, Verbeken G, Van Halle S, Boedts D, et al. Stimulation of epithelial healing in chronic postoperative otorrhea using lyophilized cultured keratinocyte lysates. Am J Otol 1997-18: 702-6.