Intracellular hepatitis C virus RNA-dependent RNA polymerase activity

Journal of Virological Methods

Volumn 113, Issue 1, 2003, Pages 65-68

  DeMarini, Douglas J ^a   Johnston, Victor K ^a   Konduri, Madhavi ^a   Gutshall, Lester L ^a   Sarisky, Robert T ^a,b  

^a GLAXOSMITHKLINE   (United States)

^b CENTOCOR INC   (United States)

Author keywords

Hepatitis C virus; Huh7 cell; RNA dependent RNA polymerase

Indexed keywords

HELICASE; RNA DIRECTED RNA POLYMERASE; VIRUS PROTEIN; VIRUS RNA;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; HEPATITIS C VIRUS; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RNA STRUCTURE; RNA SYNTHESIS; RNA TRANSCRIPTION; VIRUS GENOME; YEAST CELL;

HEPATITIS C VIRUS;

EID: 0142091667     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-0934(03)00226-X     Document Type: Article

References (24)

1. Aoki Y., Aizaki H., Shimoike T., Tani H., Ishii K., Saito I., Matsuura Y., Miyamura T. A human liver cell line exhibits efficient translation of HCV RNAs produced by a recombinant adenovirus expressing T7 RNA polymerase. Virology. 250:1998;140-150.
2. Ausubel, F.M., Brent, R., Kingston, R., Moore, D., Seidman, J., Smith, J.A., Struhl, K., 1999. Current Protocols in Molecular Biology. Wiley, New York.
3. Bartenschlager R., Ahlborn-Laake L., Mous J., Jacobsen H. Nonstructural protein 3 of the hepatitis C virus encodes a serine-type proteinase required for cleavage at the NS3/4 and NS4/5 junctions. J. Virol. 67:1993;3835-3844.
4. Behrens S.-E., Tomei L., De Francesco R. Identification and properties of the RNA-dependent RNA polymerase of hepatitis C virus. EMBO J. 15:1996;12-22.
5. Blight K.J., Kolykhalov A.A., Rice C.M. Efficient initiation of HCV RNA replication in cell culture. Science. 290:2000;1972-1974.
6. Choo Q.L., Kuo G., Weiner A.J., Overby L.R., Bradley D.W., Houghton M. Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome. Science. 244:1989;359-362.
7. Geitz D., St. Jean A., Woods R.A., Schiestl R.H. Improved method for high efficiency transformation of intact yeast cells. Nucl. Acids Res. 20:1992;1425.
8. Grakoui A., Wychowski C., Lin C., Feinstone S.M., Rice C.M. Expression and identification of hepatitis C virus polyprotein cleavage products. J. Virol. 67:1993;1385-1395.
9. Kao C., Del Vecchio A., Zhong W. De novo initiation of RNA synthesis by a recombinant flavivirus RNA-dependent RNA polymerase. Virology. 253:1999;1-7.
10. Kao C.C., Yang X., Kline A., Wang Q.M., Barket D., Heinz B.A. Template requirements for RNA synthesis by a recombinant hepatitis C virus RNA-dependent RNA polymerase. J. Virol. 74:2000;11121-11128.
11. Kato N., Hijikata M., Ootsuyama Y., Nakagawa M., Ohkoshi S., Sugimura T., Shimotohno K. Molecular cloning of the human hepatitis C virus genome from Japanese patients with non-A, non-B hepatitis. Proc. Natl. Acad. Sci. U.S.A. 87:1990;9524-9528.
12. Kim D.W., Gwack Y., Han J.H., Choe J. C-terminal domain of the hepatitis C virus NS3 protein contains an RNA helicase activity. Biochem. Biophys. Res. Commun. 215:1995;160-166.
13. Kolykhalov A.A., Mihalik K., Feinstone S., Rice C.M. Hepatitis C virus-encoded enzymatic activities and conserved RNA elements in the 3′ nontranslated region are essential for virus replication in vivo. J. Virol. 74:2000;2046-2051.
14. Lin C., Lindenbach B.D., Pragai B.M., McCourt D.W., Rice C.M. Processing in the hepatitis C virus E2-NS2 region: identification of p7 and two distinct E2-specific products with different C termini. J. Virol. 68:1994;5063-5073.
15. Lohmann V., Korner F., Herian U., Bartenschlager R. Biochemical properties of hepatitis C virus NS5B RNA-dependent RNA polymerase and identification of amino acid sequence motifs essential for enzymatic activity. J. Virol. 71:1997;8416-8428.
16. Lohmann V., Korner F., Koch J.-O., Herian U., Theilmann L., Bartenschlager R. Replication of subgenomic hepatitis C virus RNAs in a hepatoma cell line. Science. 285:1999;110-113.
17. Luo G., Hamatake R.K., Mathis D.M., Racela J., Rigat K.L., Lemm J., Colonno R.J. De novo initiation of RNA synthesis by the RNA-dependent RNA polymerase (NS5B) of hepatitis C virus. J. Virol. 74:2000;851-863.
18. McGuinness P.H., Bishop G.A., McCaughan G.W., Trowbridge R., Gowans E.J. False detection of negative-strand hepatitis C virus RNA. Lancet. 343:1994;551-552.
19. Oh J.-W., Sheu G.-T., Lai M.M.C. Template requirement and initiation site selection by hepatitis C virus polymerase on a minimal viral RNA template. J. Biol. Chem. 275:2000;17710-17717.
20. Oldenburg K.R., Vo K.T., Micahelis S., Paddon C. Recombination-mediated PCR-directed plasmid construction in vivo in yeast. Nucl. Acids Res. 25:1997;451-452.
21. Selby M.J., Glazer E., Masiarz F., Houghton M. Complex processing and protein:protein interactions in the E2:NS2 region of HCV. Virology. 204:1994;114-122.
22. Spellman P.T., Sherlock G., Zhang M.Q., Iyer V.R., Anders K., Eisen M.B., Brown P.O., Botstein D., Futcher B. Comprehensive identification of cell cycle-regulated genes of the yeast Saccharomyces cerevisiae by microarray hybridization. Mol. Cell. Biol. 9:1998;3273-3297.
23. Suzich J.A., Tamura J.K., Palmer-Hill F., Warrener P., Grakoui A., Rice C.M., Feinstone S.M., Collett M.S. Hepatitis C virus NS3 protein polynucleotide-stimulated nucleoside triphosphatase and comparison with the related pestivirus and flavivirus enzymes. J. Virol. 67:1993;6152-6158.
24. Takamizawa A., Mori C., Fuki I., Manabe S., Fujita J., Onishi E., Andoh T., Yoshida I., Okayama H. Structure and organization of the hepatitis C virus genome isolated from human carriers. J. Virol. 65:1991;1105-1113.