DNA immunization followed by a single boost with cells: A protein-free immunization protocol for production of monoclonal antibodies against the native form of membrane proteins

Journal of Immunological Methods

Volumn 280, Issue 1-2, 2003, Pages 59-72

  Nagata, Satoshi ^a   Salvatore, Giuliana ^a,b   Pastan, Ira ^a  

^a NATIONAL CANCER INSTITUTE   (United States)

^b UNIVERSITY OF NAPLES FEDERICO II   (Italy)

Author keywords

CD30; DNA immunization; Hybridoma; Immunotherapy; Monoclonal antibody; Ret

Indexed keywords

ANTIGEN; CD30 ANTIGEN; COMPLEMENTARY DNA; DNA VACCINE; EPITOPE; IMMUNOGLOBULIN G2; IMMUNOGLOBULIN G3; MEMBRANE PROTEIN; MONOCLONAL ANTIBODY; PLASMID DNA; PROTEIN RET; PROTEIN TYROSINE KINASE;

ANIMAL CELL; ARTICLE; CELL FUSION; CLINICAL PROTOCOL; COMPARATIVE STUDY; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; EXPERIMENT; FEMALE; FLUORESCENCE ACTIVATED CELL SORTING; GENE MAPPING; IMMUNIZATION; MOUSE; NONHUMAN; PRIORITY JOURNAL; WESTERN BLOTTING;

ANIMALIA;

EID: 0142075307     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(03)00192-3     Document Type: Article

References (54)

1. Andersson J., Melchers F. The antibody repertoire of hybrid cell lines obtained by fusion of X63-AG8 myeloma cells with mitogen-activated B-cell blasts. Curr. Top. Microbiol. Immunol. 81:1978;130.
2. Barry, M.A., Barry, M.E., Johnston, S.A., 1994. Production of monoclonal antibodies by genetic immunization. BioTechniques 16, 616 and 620.
3. Bowen M.A., Olsen K.J., Cheng L., Avila D., Podack E.R. Functional effects of CD30 on a large granular lymphoma cell line, YT. Inhibition of cytotoxicity, regulation of CD28 and IL-2R, and induction of homotypic aggregation. J. Immunol. 151:1993;5896.
4. Boyle J.S., Silva A., Brady J.L., Lew A.M. DNA immunization: induction of higher avidity antibody and effect of route on T cell cytotoxicity. Proc. Natl. Acad. Sci. U. S. A. 94:1997;14626.
5. Carter P. Improving the efficacy of antibody-based cancer therapies. Nat. Rev., Cancer. 1:2001;118.
6. Chowdhury P.S., Pastan I. Analysis of cloned Fvs from a phage display library indicates that DNA immunization can mimic antibody response generated by cell immunizations. J. Immunol. Methods. 231:1999;83.
7. Chowdhury P.S., Viner J.L., Beers R., Pastan I. Isolation of a high-affinity stable single-chain Fv specific for mesothelin from DNA-immunized mice by phage display and construction of a recombinant immunotoxin with anti-tumor activity. Proc. Natl. Acad. Sci. U. S. A. 95:1998;669.
8. Chowdhury P.S., Gallo M., Pastan I. Generation of high titer antisera in rabbits by DNA immunization. J. Immunol. Methods. 249:2001;147.
9. Costagliola S., Rodien P., Many M.C., Ludgate M., Vassart G. Genetic immunization against the human thyrotropin receptor causes thyroiditis and allows production of monoclonal antibodies recognizing the native receptor. J. Immunol. 160:1998;1458.
10. Deck R.R., DeWitt C.M., Donnelly J.J., Liu M.A., Ulmer J.B. Characterization of humoral immune responses induced by an influenza hemagglutinin DNA vaccine. Vaccine. 15:1997;71.
11. Donnelly J.J., Ulmer J.B., Shiver J.W., Liu M.A. DNA vaccines. Annu. Rev. Immunol. 15:1997;617.
12. Engert A., Burrows F., Jung W., Tazzari P.L., Stein H., Pfreundschuh M., Diehl V., Thorpe P. Evaluation of ricin A chain-containing immunotoxins directed against the CD30 antigen as potential reagents for the treatment of Hodgkin's disease. Cancer Res. 50:1990;84.
13. Falini B., Pileri S., Pizzolo G., Durkop H., Flenghi L., Stirpe F., Martelli M.F., Stein H. CD30 (Ki-1) molecule: a new cytokine receptor of the tumor necrosis factor receptor superfamily as a tool for diagnosis and immunotherapy. Blood. 85:1995;1.
14. Feltquate D.M., Heaney S., Webster R.G., Robinson H.L. Different T helper cell types and antibody isotypes generated by saline and gene gun DNA immunization. J. Immunol. 158:1997;2278.
15. Friguet B., Chaffotte A.F., Djavadi-Ohaniance L., Goldberg M.E. Measurements of the true affinity constant in solution of antigen-antibody complexes by enzyme-linked immunosorbent assay. J. Immunol. Methods. 77:1985;305.
16. Glennie M.J., Johnson P.W. Clinical trials of antibody therapy. Immunol. Today. 21:2000;403.
17. Goding J.W. Antibody production by hybridomas. J. Immunol. Methods. 39:1980;285.
18. Gruss H.J., Boiani N., Williams D.E., Armitage R.J., Smith C.A., Goodwin R.G. Pleiotropic effects of the CD30 ligand on CD30-expressing cells and lymphoma cell lines. Blood. 83:1994;2045.
19. Gurunathan S., Klinman D.M., Seder R.A. DNA vaccines: immunology, application, and optimization. Annu. Rev. Immunol. 18:2000;927.
20. Harlow E., Lane P. Monoclonal antibodies. Antibodies - A Laboratory Manual. 1988;139 Cold Spring Harbor Laboratory, New York.
21. Hecht T.T., Longo D.L., Cossman J., Bolen J.B., Hsu S.M., Israel M., Fisher R.I. Production and characterization of a monoclonal antibody that binds Reed-Sternberg cells. J. Immunol. 134:1985;4231.
22. Horn-Lohrens O., Tiemann M., Lange H., Kobarg J., Hafner M., Hansen H., Sterry W., Parwaresch R.M., Lemke H. Shedding of the soluble form of CD30 from the Hodgkin-analogous cell line L540 is strongly inhibited by a new CD30-specific antibody (Ki-4). Int. J. Cancer. 60:1995;539.
23. Kanegae Y., Lee G., Sato Y., Tanaka M., Nakai M., Sakaki T., Sugano S., Saito I. Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res. 23:1995;3816.
24. Kilpatrick K.E., Cutler T., Whitehorn E., Drape R.J., Macklin M.D., Witherspoon S.M., Singer S., Hutchins J.T. Gene gun delivered DNA-based immunizations mediate rapid production of murine monoclonal antibodies to the Flt-3 receptor. Hybridoma. 17:1998;569.
25. Kilpatrick K.E., Danger D.P., Hull-Ryde E.A., Dallas W. High-affinity monoclonal antibodies to PED/PEA-15 generated using 5 microg of DNA. Hybridoma. 19:2000;297.
26. Kohler G., Milstein C. Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion. Eur. J. Immunol. 6:1976;511.
27. Koon H.B., Junghans R.P. Anti-CD30 antibody-based therapy. Curr. Opin. Oncol. 12:2000;588.
28. Krasemann S., Jurgens T., Bodemer W. Generation of monoclonal antibodies against prion proteins with an unconventional nucleic acid-based immunization strategy. J. Biotechnol. 73:1999;119.
29. Kreitman R.J., Bailon P., Chaudhary V.K., FitzGerald D.J., Pastan I. Recombinant immunotoxins containing anti-Tac(Fv) and derivatives of Pseudomonas exotoxin produce complete regression in mice of an interleukin-2 receptor-expressing human carcinoma. Blood. 83:1994;426.
30. Kreitman R.J., Wilson W.H., White J.D., Stetler-Stevenson M., Jaffe E.S., Giardina S., Waldmann T.A., Pastan I. Phase I trial of recombinant immunotoxin anti-Tac(Fv)-PE38 (LMB-2) in patients with hematologic malignancies. J. Clin. Oncol. 18:2000;622.
31. Kreitman R.J., Wilson W.H., Bergeron K., Raggio M., Stetler-Stevenson M., FitzGerald D.J., Pastan I. Efficacy of the anti-CD22 recombinant immunotoxin BL22 in chemotherapy-resistant hairy-cell leukemia. N. Engl. J. Med. 345:2001;241.
32. Manie S., Santoro M., Fusco A., Billaud M. The RET receptor: function in development and dysfunction in congenital malformation. Trends Genet. 17:2001;580.
33. Mansfield E., Amlot P., Pastan I., FitzGerald D.J. Recombinant RFB4 immunotoxins exhibit potent cytotoxic activity for CD22-bearing cells and tumors. Blood. 90:1997;2020.
34. Matsuura Y., Tatsumi M., Enami K., Morikawa S., Yamazaki S., Kohase M. Expression of IL-6/IFN-beta 2 in a baculovirus system and its biological function. Ann. N.Y. Acad. Sci. 557:1989;122.
35. Moonsom S., Khunkeawla P., Kasinrerk W. Production of polyclonal and monoclonal antibodies against CD54 molecules by intrasplenic immunization of plasmid DNA encoding CD54 protein. Immunol. Lett. 76:2001;25.
36. Nagata S., Yamamoto K., Ueno Y., Kurata T., Chiba J. Preferential generation of monoclonal IgG-producing hybridomas by use of vesicular stomatitis virus-mediated cell fusion. Hybridoma. 10:1991;369.
37. Nagata S., Onda M., Numata Y., Santora K., Beers R., Kreitman R.J., Pastan I. Novel anti-CD30 recombinant immunotoxins containing disulfide-stabilized Fv fragments. Clin. Cancer Res. 8:2002;2345.
38. Oi V.T., Herzenberg L.A. Immunoglobulin-producing hybrid cell lines. Mishell B.B., Shiigi S.M. Selected Methods in Cellular Immunology. 1980;351 Freeman, San Francisco.
39. Pastan I., Kreitman R.J. Immunotoxins in cancer therapy. Curr. Opin. Investig. Drugs. 3:2002;1089.
40. Pertmer T.M., Roberts T.R., Haynes J.R. Influenza virus nucleoprotein-specific immunoglobulin G subclass and cytokine responses elicited by DNA vaccination are dependent on the route of vector DNA delivery. J. Virol. 70:1996;6119.
41. Raz E., Carson D.A., Parker S.E., Parr T.B., Abai A.M., Aichinger G., Gromkowski S.H., Singh M., Lew D., Yankauckas M.A., Baird S.M., Rhodes G.H. Intradermal gene immunization: the possible role of DNA uptake in the induction of cellular immunity to viruses. Proc. Natl. Acad. Sci. U. S. A. 91:1994;9519.
42. Rozemuller H., Chowdhury P.S., Pastan I., Kreitman R.J. Isolation of new anti-CD30 scFvs from DNA-immunized mice by phage display and biologic activity of recombinant immunotoxins produced by fusion with truncated Pseudomonas exotoxin. Int. J. Cancer. 92:2001;861.
43. Salvatore G., Nagata S., Billaud M., Santoro M., Vecchio G., Pastan I. Generation and characterization of novel monoclonal antibodies to the Ret receptor tyrosine kinase. Biochem. Biophys. Res. Commun. 294:2002;813.
44. Santoro M., Carlomagno F., Romano A., Bottaro D.P., Dathan N.A., Grieco M., Fusco A., Vecchio G., Matoskova B., Kraus M.H., DiFiore P.P. Activation of RET as a dominant transforming gene by germline mutations of MEN2A and MEN2B. Science. 267:1995;381.
45. Schmolke S., Tacke M., Schmitt U., Engel A.M., Ofenloch-Haehnle B. Identification of hepatitis G virus particles in human serum by E2-specific monoclonal antibodies generated by DNA immunization. J. Virol. 72:1998;4541.
46. Schwab U., Stein H., Gerdes J., Lemke H., Kirchner H., Schaadt M., Diehl V. Production of a monoclonal antibody specific for Hodgkin and Sternberg-Reed cells of Hodgkin's disease and a subset of normal lymphoid cells. Nature. 299:1982;65.
47. Schwarting R., Gerdes J., Durkop H., Falini B., Pileri S., Stein H. BER-H2: a new anti-Ki-1 (CD30) monoclonal antibody directed at a formol-resistant epitope. Blood. 74:1989;1678.
48. Shulman M., Wilde C.D., Kohler G. A better cell line for making hybridomas secreting specific antibodies. Nature. 276:1978;269.
49. Tang D.C., DeVit M., Johnston S.A. Genetic immunization is a simple method for eliciting an immune response. Nature. 356:1992;152.
50. Tearina Chu T.H., Halverson G.R., Yazdanbakhsh K., Oyen R., Reid M.E. A DNA-based immunization protocol to produce monoclonal antibodies to blood group antigens. Br. J. Haematol. 113:2001;32.
51. Ulivieri C., Burroni D., Telford J.L., Baldari C.T. Generation of a monoclonal antibody to a defined portion of the Helicobacter pylori vacuolating cytotoxin by DNA immunization. J. Biotechnol. 51:1996;191.
52. Velikovsky C.A., Cassataro J., Sanchez M., Fossati C.A., Fainboim L., Spitz M. Single-shot plasmid DNA intrasplenic immunization for the production of monoclonal antibodies. Persistent expression of DNA. J. Immunol. Methods. 244:2000;1.
53. Wolff J.A., Malone R.W., Williams P., Chong W., Acsadi G., Jani A., Felgner P.L. Direct gene transfer into mouse muscle in vivo. Science. 247:1990;1465.
54. Wolff J.A., Williams P., Acsadi G., Jiao S., Jani A., Chong W. Conditions affecting direct gene transfer into rodent muscle in vivo. BioTechniques. 11:1991;474.