Generation of monoclonal antibodies against prion proteins with an unconventional nucleic acid-based immunization strategy

Journal of Biotechnology

Volumn 73, Issue 2-3, 1999, Pages 119-129

  Krasemann, Susanne ^a   Jürgens, Thomas ^b   Bodemer, Walter ^b  

^a Laboratory of Molecular Genetic   (Germany)

^b UNIVERSITY OF ROSTOCK   (Germany)

Author keywords

Monoclonal antibodies; Prion proteins; PrP(0 0) mice

Indexed keywords

CLONING; DIAGNOSIS; DISEASES; DNA; IMMUNIZATION; IMMUNOLOGY; NUCLEIC ACIDS; RNA; TISSUE;

CREUTZFELDT-JAKOB DISEASE; FATAL FAMILIAL INSOMNIA; GERTSMANN-STRAUSSLER-SCHEINKER SYNDROME; IMMUNOFLUORESCENCE; IMMUNOPRECIPITATION; MICE; PATHOMECHANISM; PEPTIDE ELISA; WESTERN BLOT;

MONOCLONAL ANTIBODIES;

EPITOPE; MONOCLONAL ANTIBODY; MUTANT PROTEIN; NUCLEIC ACID; PLASMID DNA; PRION PROTEIN; RNA;

AMINO ACID SEQUENCE; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; CELL STRAIN BHK; CLONING VECTOR; CONFERENCE PAPER; CONTROLLED STUDY; CREUTZFELDT JAKOB DISEASE; ENZYME LINKED IMMUNOSORBENT ASSAY; FATAL FAMILIAL INSOMNIA; GERSTMANN STRAUSSLER SCHEINKER SYNDROME; HUMAN; HYBRIDOMA; IMMUNE RESPONSE; IMMUNIZATION; IMMUNOBLOTTING; IMMUNOFLUORESCENCE; IMMUNOPRECIPITATION; KURU; MOUSE; NONHUMAN; PRION DISEASE; PRIORITY JOURNAL; PROTEIN BINDING; SEMLIKI FOREST ALPHAVIRUS; VIRUS RECOMBINANT;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, MONOCLONAL; BIOTECHNOLOGY; CELL LINE; DNA; EPITOPES; GENETIC VECTORS; HUMANS; IMMUNIZATION; MICE; MOLECULAR SEQUENCE DATA; PRION DISEASES; PRIONS; RNA; SEMLIKI FOREST VIRUS; TRANSFECTION;

ANIMALIA; SEMLIKI FOREST VIRUS;

EID: 0345540635     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(99)00115-7     Document Type: Conference Paper

References (24)

1. Berglund P., Sjöberg M., Garoff H., Atkins G.J., Sheahan B.J., Liljeström P. Semliki Forest virus expression system: production of conditionally infectious recombinant particles. Bio/Technology. 11:1993;916-920.
2. Brown P., Goldfarb L.G., Gajdusek C.D. The new biology of spongiform encephalopathy: infectious amyloidoses with a genetic twist. Lancet. 337:1991;1019-1022.
3. Brown P., Cervenakowa L., Goldfarb L.G. et al. Iatrogenic Creutzfeldt-Jakob disease: an example of the interplay between ancient genes and modern medicine. Neurology. 44:1994;291-293.
4. Brown D.R., Qin K., Herms J.W. et al. The cellular prion protein binds copper in vivo. Nature. 390:1997;684-687.
5. Büeler H., Fischer M., Lang Y. et al. Normal development and behaviour of mice lacking the neuronal cell-surface PrP protein. Nature. 356:1992;577-582.
6. Büeler H., Aguzzi A., Sailer A. et al. Mice devoid of PrP are resistant to scrapie. Cell. 73:1993;1339-1347.
7. Caughey B., Raymond G.J., Ernst D., Race R.E. N-terminal truncation of the scrapie-associated form of PrP by lysosomal protease(s): implications regarding the site of conversion of PrP to the protease-resistant state. J. Virol. 65:1991;6597-6603.
8. Davis H.L., Michel M.-L., Mancini M., Schleef M., Whalen R.G. Direct gene transfer in skeletal muscle: plasmid DNA-based immunization against the hepatitis B surface antigen. Vaccine. 12:1994;1503-1509.
9. Kearny J.F., Radbruch A., Liesegang B., Rajewski K. A mouse myeloma cell line that has lost immunglobulin expression but permits the construction of antibody-secreting hybridoma cell lines. J. Immunol. 123:1979;1548-1558.
10. Korth C., Stierli B., Streit P. et al. Prion (PrPSc)-specific epitope defined by a monoclonal antibody. Nature. 390:1997;74-77.
11. Krasemann S., Zerr I., Weber T. et al. Prion disease associated with a novel nine octapeptide repeat-insertion in the PRNP-gene. Mol. Brain Res. 34:1995;173-176.
12. 0/0-mice. J. Immunol. Methods. 199:1996;109-118.
13. Liljeström P., Garoff H. A new generation of animal cell expression vectors based on the Semliki Forest virus replicon. Bio/Technology. 9:1991;1356-1361.
14. Meyer R.K., McKinley M.P., Bowman K.A., Braunfeld M.B., Barry R.A., Prusiner S.B. Seperation and properties of cellular and scrapie prion proteins. Proc. Natl. Acad. Sci. 83:1986;2310-2314.
15. Pan K.-M., Baldwin M., Nguyen J. et al. Conversion of alpha-helices into beta-sheets features in the formation of the scrapie prion proteins. Proc. Natl. Acad. Sci. 90:1993;10962-10966.
16. Peters, J.H., Baumgarten, H., 1990. Monoclonal Antibodies, 2nd edn., Springer, Berlin, pp. 150-157.
17. Prusiner S.B. Novel proteinacious infectious particles cause scrapie. Science. 216:1982;136-144.
18. Prusiner S.B. Molecular biology of prion diseases. Science. 252:1991;1515-1522.
19. Prusiner S.B., Hsiao K.K. Human prion diseases. Ann. Neurol. 35:1994;385-395.
20. Prusiner S.B., Groth D., Serban A. et al. Ablation of the prion protein (PrP) gene in mice prevents scrapie and facilitates production of anti-PrP antibodies. Proc. Natl. Acad. Sci. 90:1993;10608-10612.
21. Robakis N.K., Devine-Gage E.A., Jenkins E.C. et al. Localization of a human gene homologous to the PrP gene on the arm of chromosome and detection of PrP-related antigens in normal human brains. Biochem. Biophys. Res. Commun. 140:1986;758-765.
22. Stahl N., Baldwin M.A., Teplow D. et al. Cataloguing post-translational modifications of the scrapie prion protein by mass spectroscopy. Prusiner S.B., Collinge J., Powell J., Anderton B. Prion Diseases of Humans and Animals. 1992;361-379 Ellis Horwood, London.
23. Ulmer J.B., Donelly J.J., Parker S.E. et al. Heterologous protection against influenza by injection of DNA encoding a viral protein. Science. 259:1993;1745-1749.
24. Xiang Z.Q., Spitalnik S., Tran M., Wunner W.H., Cheng J., Ertl H.C. Vaccination with a plasmid vector carrying the rabies virus glycoprotein gene induces protective immunity against rabies virus. Virology. 199:1994;132-140.