Detection of DNA lesions induced by chemical mutagens using the single-cell gel electrophoresis (Comet) assay. 2. Relationship between DNA migration and alkaline condition

Mutation Research - Genetic Toxicology and Environmental Mutagenesis

Volumn 393, Issue 1-2, 1997, Pages 107-113

  Miyamae, Youichi ^a   Iwasaki, Kouichi ^b   Kinae, Naohide ^b   Tsuda, Shuji ^c   Murakami, Michiko ^d   Tanaka, Makiko ^d   Sasaki, Yu F ^d  

^a ASTELLAS PHARMA INC   (Japan)

^b UNIVERSITY OF SHIZUOKA   (Japan)

^c IWATE UNIVERSITY   (Japan)

^d HACHINOHE NATIONAL COLLEGE OF TECHNOLOGY   (Japan)

Author keywords

Alkaline single cell electrophoresis (SCG) assay; Chemical mutagen; Comet assay; DNA lesion; pH

Indexed keywords

4 NITROQUINOLINE 1 OXIDE; BLEOMYCIN; CHEMICAL MUTAGEN; ETHYLNITROSOUREA; MESYLIC ACID ETHYL ESTER; MESYLIC ACID METHYL ESTER; N METHYL N NITROSOURETHAN;

ANIMAL CELL; ARTICLE; CHO CELL; CONTROLLED STUDY; DNA DAMAGE; GEL ELECTROPHORESIS; MUTAGENICITY; NONHUMAN; PRIORITY JOURNAL;

ALKYLATING AGENTS; ANIMALS; CHO CELLS; CRICETINAE; CROSS-LINKING REAGENTS; DNA; DNA DAMAGE; ELECTROPHORESIS; EVALUATION STUDIES; HYDROGEN-ION CONCENTRATION; MUTAGENICITY TESTS; MUTAGENS;

ANIMALIA;

EID: 0030725479     PISSN: 13835718     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1383-5718(97)00091-0     Document Type: Article

References (18)

1. Singh N.P., McCoy M.T., Tice R.R., Schneider E.L. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp. Cell. Res. 175:1988;184-191.
2. Fairbairn D.W., Olive P.L., O'Neill K.L. The comet assay: a comprehensive review. Mutation Res. 339:1995;37-59.
3. E.C. Friedberg, DNA Repair, Freeman Co., New York, 1984.
4. Olive P.L., Wlodek D., Durand R.E., Banath J.P. Factors influencing DNA migration from individual cells subjected to gel electrophoresis. Exp. Cell Res. 198:1992;259-267.
5. Pfuhler S., Wolf H.U. Detection of DNA-crosslinking agents with the alkaline comet assay. Environ. Mol. Mutagen. 27:1996;196-201.
6. Vijayalaxmi R., Tice R., Strauss G.H.S. Assessment of radiation-induced DNA damage in human lymphocytes using single cell gel electrophoresis technique. Mutation Res. 271:1992;243-252.
7. Sasaki Y.F., Yamada H., Sugiyama C., Kinae N. Increasing effects of tri-n-butyltins and triphenyltins on the frequency of chemically induced chromosome aberrations in cultured Chinese hamster cells. Mutation Res. 300:1993;5-14.
8. Sasaki Y.F., Sakaguchi M., Yamagishi T., Yamada H., Shirasu Y. Bio-anticlastogenic effects of unsaturated fatty acids included in fish oil - docosahaxaenoic acid, docosapentaenoic acid, and eicosapentaenoic acid - in cultured Chinese hamster cells. Mutation Res. 320:1994;9-22.
9. Sasaki Y.F., Kinae N. Co-clastogenic effects of water pollutants. Environ. Mutation Res. Commun. 17:1995;65-74.
10. Vig B.K., Lewis R. Genetic toxicology of bleomycin. Mutation Res. 49:1978;189-238.
11. Friedberg E.C., Ehmann U.K., Williams J.I. Human diseases associated with defective DNA repair. Adv. Radiat. Biol. 8:1979;85-174.
12. Hanawalt P.C., Cooper P.K., Ganesan A.K., Smith C.A. DNA repair in bacteria and mammalian cells. Annu. Rev. Biochem. 48:1979;783-803.
13. Hyttinen J.M.T., Jansson K. PM2 DNA damage induced by 3-chloro-4-(dichloromethyl)-5-hydroxy-2-(5H)-furanone (MX). Mutation Res. 348:1995;183-186.
14. Szybalski W. Crosslinking of DNA by enzymatically or chemically activated mitomycins and porfiromycins, bifunctionally alkylating antibiotics. Fed. Proc. 23:1964;946-957.
15. Sasaki M.S., Tonomura A. A high susceptibility in Fanconi's anemia to chromosome breakage by DNA crosslinking agents. Cancer Res. 33:1973;1829-1836.
16. Sasaki M.S. Is Fanconi's anemia defective in a process essential for the repair of DNA crosslinks? Nature. 257:1975;501-503.
17. M.S. Sasaki, Fanconi's anemia: a condition possibly associated with defective DNA repair. In: P.C. Hanawalt, E.C. Friedberg, C.F. Fox (Eds.), DNA Repair Mechanisms, Academic Press, New York, 1978, pp. 675-684.
18. Miyamae Y., Zaizen K., Ohara K., Mine Y., Sasaki Y.F. Detection of DNA lesions induced by chemical mutagens by the single cell gel electrophoresis (Comet) assay. 1. Relationship between the onset of DNA damage and the characteristics of mutagens. Mutation Res. 393:1997;99-106.