Three-dimensional co-culture of primary human liver cells in bioreactors for in vitro drug studies: Effects of the initial cell quality on the long-term maintenance of hepatocyte-specific functions

ATLA Alternatives to Laboratory Animals

Volumn 30, Issue 5, 2002, Pages 525-538

  Zeilinger, Katrin ^a   Sauer, Igor M ^a   Pless, Gesine ^a   Strobel, Catrin ^a   Rudzitis, Jeannette ^a   Wang, Aiguo ^a   Nüssler, Andres K ^a   Grebe, Alexander ^a   Mao, Lei ^a   Auth, Stefan H G ^a   Unger, Juliane ^a   Neuhaus, Peter ^a   Gerlach, Jörg C ^a  

^a HUMBOLDT UNIVERSITY   (Germany)

Author keywords

Bioreactor; Co culture; Cytochrome P450; Human liver cells; In vitro culture

Indexed keywords

CYTOCHROME P450; ETHOXYRESORUFIN DEETHYLASE; GLUCOSE; PROTEIN; UREA; ALBUMINOID; ASPARTATE AMINOTRANSFERASE; CARBON DIOXIDE; CYTOCHROME P450 1A1; LACTATE DEHYDROGENASE; LIDOCAINE; OXYGEN; TESTOSTERONE;

ARTICLE; BIOREACTOR; CELL DIFFERENTIATION; CELL FUNCTION; COCULTURE; CONTROLLED STUDY; CULTURE MEDIUM; DRUG INDUSTRY; DRUG RESEARCH; ENZYME ACTIVITY; ENZYME INDUCTION; GLUCOSE METABOLISM; HUMAN; HUMAN CELL; LIVER CELL CULTURE; LIVER METABOLISM; LIVER PRESERVATION; LIVER RESECTION; MICROSCOPY; OXYGENATION; PRIORITY JOURNAL; PROTEIN SYNTHESIS; UREA CYCLE; ANIMAL TESTING ALTERNATIVE; CELL SURVIVAL; COMPARATIVE STUDY; CYTOLOGY; DRUG EFFECT; IN VITRO STUDY; INSTRUMENTATION; LIVER; LIVER CELL; LIVER TRANSPLANTATION; METABOLISM; METHODOLOGY; ORGAN PRESERVATION; PHYSIOLOGY; SECRETION;

ALBUMINS; ANIMAL TESTING ALTERNATIVES; ASPARTATE AMINOTRANSFERASES; BIOREACTORS; CARBON DIOXIDE; CELL DIFFERENTIATION; CELL SURVIVAL; COCULTURE TECHNIQUES; CYTOCHROME P-450 CYP1A1; GLUCOSE; HEPATOCYTES; HUMANS; L-LACTATE DEHYDROGENASE; LIDOCAINE; LIVER; LIVER TRANSPLANTATION; ORGAN PRESERVATION; OXYGEN; TESTOSTERONE; UREA;

EID: 0141587163     PISSN: 02611929     EISSN: None     Source Type: Journal    
DOI: 10.1177/026119290203000506     Document Type: Article

References (33)

1. Guillouzo, A. (1992). Hepatotoxicity. In In Vitro Toxicity Testing: Applications to Safety Evaluation (ed. J.M. Frazier), pp. 45-83. Baltimore, MD, USA: Marcel Dekker.
2. Guillouzo, A. (1998). Liver cell models in in vitro toxicology. Environmental Health Perspectives 106, 511-532.
3. Donato, M.T., Castell, J.V. & Gómez-Lechón, M.J. (1999). Characterization of drug metabolizing activities in pig hepatocytes for use in bioartificial liver devices: Comparison with other hepatic cellular models. Journal of Hepatology 31, 542-549.
4. Silva, J.M., Morin, P.E., Day, S.H., Kennedy, B.P., Payette, P., Rushmore, T., Yergey, J.A. & NicollGriffith, D.A. (1998). Refinement of an in vitro cell model for cytochrome P450 induction. Drug Metabolism and Disposition 26, 490-496.
5. Bayliss, M.K., Bell, J.A., Jenner, W.N., Park, G.R. & Wilson, K. (1999). Utility of hepatocytes to model species differences in the metabolism of loxtidine and to predict pharmacokinetic parameters in rat, dog and man. Xenobiotica 29, 253-268.
6. Li, A.P., Maurel, P., Gómez-Léchon, M.J., Cheng, L.C. & Jurima-Romet, M. (1997). Preclinical evaluation of drug-drug interaction potential: Present status of the application of primary human hepatocytes in the evaluation of cytochrome P450 induction. Chemico-Biological Interactions 107, 5-16.
7. Kedderis, G.L. (1997). Extrapolation of in vitro enzyme induction data to humans in vivo. Toxicology 118, 195-204.
8. Guillouzo, A., Morel, F., Fardel, O. & Meunier, B. (1993). Use of human hepatocyte cultures for drug metabolism studies. Toxicology 82, 209-219.
9. Morel, F., Beaune, P.H., Ratanasavanh, D., Flinois, J.P., Yang, C.S., Guengerich, F-P. & Guillouzo, A. (1990). Expression of cytochrome P450 enzymes in cultured human hepatocytes. European Journal of Biochemistry 191, 437-444.
10. Runge, D., Kohler, C., Kostrubsky, V.E., Jager, D., Lehmann, T., Runge, D.M., May, U., Stolz, D.B., Strom, S.C., Fleig, W.E. & Michalopoulos, G.K. (2000). Induction of cytochrome P450 (CYP)1A1, CYP1A2 and CYP3A4 but not of CYP2C9, CYP2C19, multidrug resistance (MDR-1) and multidrug resistance associated protein (MRP-1) by prototypical inducers in human hepatocytes. Biochemical and Biophysical Research Communications 273, 333-341.
11. Bader, A., Zech, K., Crome, O., Christians, U., Ringe, B., Pichlmayr, R. & Sewing, K.F. (1994). Use of organotypical cultures of primary hepatocytes to analyse drug biotransformation in man and animals. Xenobiotica 24, 623-633.
12. Koebe, H.G., Pahernik, S., Eyer, P. & Schildberg, F.W. (1994). Collagen gel immobilization: A useful cell culture technique for long-term metabolic studies on human hepatocytes. Xenobiotica 24, 95-107.
13. Kono, Y., Yang, S. & Roberts, E.A. (1997). Extended primary culture of human hepatocytes in a collagen gel sandwich system. In Vitro Cellular and Developmental Biology - Animal 33, 467-472.
14. George, J., Goodwin, B., Liddle, C., Tapner, M. & Farrell, G.C. (1997). Time-dependent expression of cytochrome P450 genes in primary cultures of welldifferentiated human hepatocytes. Journal of Laboratory and Clinical Medicine 129, 638-648.
15. Blaheta, R.A., Kronenberger, B., Woitaschek, D., Auth, M.K., Scholz, M., Weber, S., Schuldes, H., Encke, A. & Markus, B.H. (1998). Dedifferentiation of human hepatocytes by extracellular matrix proteins in vitro: Quantitative and qualitative investigation of cytokeratin 7, 8, 18, 19 and vimentin filaments. Journal of Hepatology 28, 677-690.
16. Tong, J.Z., Sarrazin, S., Cassio, D., Gauthier, F. & Alvarez, F. (1994). Application of spheroid culture to human hepatocytes and maintenance of their differentiation. Biology of the Cell 81, 77-81.
17. Auth, M.K., Okamoto, M., Ishida, Y., Keogh, A., Auth, S.H., Gerlach, J., Encke, A., McMaster, P. & Strain, A.J. (1998). Maintained function of primary human hepatocytes by cellular interactions in coculture: Implications for liver support systems. Transplant International 11, S439-S443.
18. Gerlach, J., Encke, J., Hole, O., Müller, C., Courtney, J. & Neuhaus, P. (1994). Hepatocyte culture between three-dimensional arranged biomatrixcoated independent artificial capillary systems and sinusoidal endothelial cell co-culture compartments. International Journal of Artificial Organs 17, 301-306.
19. Gerlach, J.C., Schnoy, N., Encke, J., Smith, M.D., Müller, C. & Neuhaus, P. (1995). Improved hepatocyte in vitro maintenance in a culture model with woven multicompartment capillary systems: Electron microscopy studies. Hepatology 22, 546-552.
20. Gerlach, J., Unger, J., Hole, O., Encke, J., Müller, C. & Neuhaus, P. (1994). Bioreactor for long-term maintenance of differentiated hepatic cell functions. Alternativen zu Tierexperimenten 11, 207-215.
21. Gerlach, J. (1997). Long-term liver cell cultures in bioreactors and possible application for liver support. Cell Biology and Toxicology 13, 349-355.
22. Zeilinger, K., Auth, S., Unger, J., Grebe, A., Mao, L., Petrik, M., Holland, G., Appel, K., Nüssler, A., Neuhaus, P. & Gerlach, J. (2000). Liver cell culture in bioreactors for in vitro studies on drug metabolism as alternative to animal experiments. Alternativen zu Tierexperimenten 17, 3-10.
23. Nüssler, A.K., Wang, A., Neuhaus, P., Fischer, J., Yuan, J., Liu, L., Zeilinger, K., Gerlach, J., Arnold, P.J. & Albrecht, W. (2001). The suitability of hepatocyte culture models to study various aspects of drug metabolism. Alternativen zu Tierexperimenten 18, 91-101.
24. Belzer, F.O. & Southard, J.H. (1988). Principles of solid-organ preservation by cold storage. Transplantation 45, 673-676.
25. Dorko, K., Freeswick, P.D., Bartol, F., Cicalese, L., Barsley, B.A., Tzakis, A. & Nüssler, A.K. (1994). A new technique for isolating and culturing human hepatocytes from whole or split livers not used for transplantation. Cell Transplantation 3, 387-395.
26. Gerlach, J.C., Brambacher, J., Kloppel, K., Schnoy, N. & Neuhaus, P. (1994). Comparison of four methods for mass hepatocyte isolation from pig and human livers. Transplantation 57, 1318-1322.
27. Donato, M.T., Castell, J.V. & Gómez-Lechón, M.J. (1994). Cytochrome P450 activities in pure and cocultured rat hepatocytes: Effect of model inducers. In Vitro Cellular and Developmental Biology - Animal 30, 825-832.
28. Burke, M.D. & Hallman, H. (1978). Microfluorimetric analysis of the cytochrome P448 associated, ethoxyresorufin O-deethylase activities of individual isolated rat hepatocytes. Biochemical Pharmacology 27, 1539-1544.
29. Donato, M.T., Gómez-Lechón, M.J. & Castell, J.V. (1993). A microassay for measuring cytochrome P4501A1 and P4502B1 activities in intact human and rat hepatocytes cultured on 96-well plates. Analytical Biochemistry 213, 29-33.
30. Beken, S., De Smet, K., Depreter, M., Roels, F., Vercruysse, A. & Rogiers, V. (2001). Effects of L-proline on phase I and phase II xenobiotic biotransformation capacities of rat and human hepatocytes in long-term collagen gel cultures. ATLA 29, 35-53.
31. Iqbal, S., Elcombe, C-R. & Elias, E. (1991). Maintenance of mixed-function oxidase and conjugation enzyme activities in hepatocyte cultures prepared from normal and diseased human liver. Journal of Hepatology 12, 336-343.
32. Ploeg, R.J., D'Alessandro, A.M., Knechtle, S.J., Stegall, M.D., Pirsch, J.D., Hoffmann, R.M., Sasaki, T., Sollinger, H.W., Belzer, F.O. & Kalayoglu, M. (1993). Risk factors for primary dysfunction after liver transplantation: A multivariate analysis. Transplantation 55, 807-813.
33. Strasberg, S.M., Howard, T.K., Molmenti, E.P. & Hertl, M. (1994). Selecting the donor liver: Risk factors for poor function after orthotopic liver transplantation. Hepatology 20, 829-838.