Dedifferentiation of human hepatocytes by extracellular matrix proteins in vitro: Quantitative and qualitative investigation of cytokeratin 7, 8, 18, 19 and vimentin filaments

Journal of Hepatology

Volumn 28, Issue 4, 1998, Pages 677-690

  Blaheta, Roman A ^a   Kronenberger, Bernd ^a   Woitaschek, Dirk ^a   Auth, Marcus K ^a   Scholz, Martin ^a   Weber, Stephan ^a   Schuldes, Horst ^a   Encke, Albrecht ^a   Markus, Bernd H ^a  

^a UNIVERSITY HOSPITAL FRANKFURT   (Germany)

Author keywords

Hepatocyte culture; Intermediate filaments; Single matrix; Three dimensional matrix

Indexed keywords

COLLAGEN TYPE 1; COLLAGEN TYPE 4; CYTOKERATIN; CYTOKERATIN 19; FIBRONECTIN; LAMININ; SCLEROPROTEIN; VIMENTIN;

ARTICLE; CELL DIFFERENTIATION; CELL STRAIN HEPG2; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; GALLBLADDER EPITHELIUM; HUMAN; HUMAN CELL; INTERMEDIATE FILAMENT; LIVER CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION;

EID: 0031961443     PISSN: 01688278     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-8278(98)80293-6     Document Type: Article

References (56)

1. Lai YS, Thung SN, Gerber MA, Chen ML, Schaffner F. Expression of cytokeratins in normal and diseased livers and in primary liver carcinomas. Arch Pathol Lab Med. 113:1989;134-138.
2. Moll R, Hage C, Thoenes W. Expression of intermediate filament proteins in fetal and adult human kidney: modulations of intermediate filament patterns during development and in damaged tissue. Lab Invest. 65:1991;74-86.
3. Moll R, Franke WW, Schiller DL, Geiger B, Krepler R. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell. 31:1982;11-24.
4. Franke WW, Mayer D, Schmid E, Denk H, Borenfreund E. Differences of expression of cytoskeletal proteins in cultured rat hepatocytes and hepatoma cells. Exp Cell Res. 134:1981;345-365.
5. Van Eyken P, Sciot R, Paterson A, Callea F, Kew MC, Desmet VJ. Cytokeratin expression in hepatocellular carcinoma: an immunohistochemical study. Hum Pathol. 19:1988;562-568.
6. Arthur MJP. Degradation of matrix proteins in liver fibrosis. Path Res Pract. 190:1994;825-833.
7. Neubauer K, Knittel T, Armbrust T, Ramadori G. Accumulation and cellular localization of fibrinogen/fibrin during short-term and long-term rat liver injury. Gastroenterology. 108:1995;1124-1135.
8. Takahara T, Furui K, Funaki J, Nakayama Y, Itoh H, Miyabayashi C, et al. Increased expression of matrix metalloproteinase-II in experimental liver fibrosis in rats. Hepatology. 21:1995;787-795.
9. Terada T, Ohta T, Minato H, Nakanuma Y. Expression of pancreatic trypsinogen/trypsin and cathepsin B in human cholangiocarcinomas and hepatocellular carcinomas. Hum Pathol. 26:1995;746-752.
10. Koivunen E, Ristimäki A, Itkonen O, Osman S, Vuento M, Stenman UH. Tumor-associated trypsin participates in cancer cell-mediated degradation of extracellular matrix. Cancer Res. 51:1991;2107-2112.
11. Liotta LA, Rao CN, Wewer UM. Biochemical interactions of tumor cells with the basement membrane. Ann Rev Biochem. 55:1986;1037-1057.
12. Tryggvason K, Höyhtyä M, Salo T. Proteolytic degradation of extracellular matrix in tumor invasion. Biochim Biophys Acta. 907:1987;191-217.
13. Chenery RJ, Ayrton A, Oldham HG, Standring P, Norman SJ, Seddon T, et al. Diazepam metabolism in cultured hepatocytes from rat, rabbit, dog, guinea pig, and man. Drug Metabol Dispos. 15:1987;312-317.
14. Clement B, Rescan PY, Baffet G, Loreal O, Lehry D, Campion JP, et al. Hepatocytes may produce laminin in fibrotic liver and in primary culture. Hepatology. 8:1988;794-803.
15. Grant MH, Burke MD, Hawksworth GM, Duthie SJ, Engeset J, Petrie JC. Human adult hepatocytes in primary monolayer culture: maintenance of mixed function oxidase and conjugation pathways of drug metabolism. Biochem Pharmacol. 36:1987;2311-2316.
16. Guillouzo A, Beaune P, Gascoin MN, Begue JM, Campion JP, Guengerich FP, et al. Maintenance of cytochrome P-450 in cultured adult human hepatocytes. Biochem Pharmacol. 34:1985;2991-2995.
17. Ismail T, Howl J, Wheatley M, McMaster P, Neuberger JM, Strain AJ. Growth of normal human hepatocytes in primary culture: effect of hormones and growth factors on DNA synthesis. Hepatology. 14:1991;1076-1082.
18. Keppens S, Vandekerckhove A, Moshage H, Yap SH, Aerts R, de Wulf H. Regulation of glycogen phosphorylase activity in isolated human hepatocytes. Hepatology. 17:1993;610-614.
19. Richard B, Fabre G, de Sousa G, Fabre I, Rahmani R, Cano JP. Interspecies variability in mitoxantrone metabolism using primary cultures of hepatocytes isolated from rat, rabbit and humans. Biochem Pharmacol. 41:1990;255-262.
20. Ryan CM, Carter EA, Jenkins RL, Sterling LM, Yarmush ML, Malt RA, et al. Isolation and long-term culture of human hepatocytes. Surgery. 113:1993;48-54.
21. Strain AJ, McGuinness G, Rubin JS, Aaronson SA. Keratinocyte growth factor and fibroblast growth factor action on DNA synthesis in rat and human hepatocytes: modulation by heparin. Exp Cell Res. 210:1994;1-7.
22. Schröder AJ, Blaheta RA, Scholz M, Encke A, Markus BH. Isolation and separation of human adult hepatocytes: yield and purity by using different methods. Zentralbl Chir. 119:1994;127-138.
23. Schröder AJ, Blaheta RA, Scholz M, Kronenberger B, Encke A, Markus BH. Effects of proinflammatory cytokines on cultivated primary human hepatocytes: fluorometric measurement of intercellular adhesion molecule-1 and human leukocyte antigen-A, -B, -C, and -DR expression. Transplantation. 59:1995;1023-1028.
24. Kleinman HK, McGarvey ML, Hassell JR, Star V, Cannon F, Laurie G, et al. Basement membrane complexes with biological activity. Biochemistry. 25:1986;312-318.
25. Orkin RW, Gehron P, McGoodwin EB, Martin GR, Valentine T, Swarm R. A murine tumor producing a matrix of basement membrane. J Exp Med. 145:1977;204-220.
26. Reid LM, Fiorino AS, Sigal SH, Brill S, Holst PA. Extracellular matrix gradients in the space of Disse: relevance to liver biology. Hepatology. 15:1992;1198-1203.
27. Dunn JCY, Tompkins RG, Yarmush ML. Long-term in vitro function of adult hepatocytes in a collagen sandwich configuration. Biotechnol Prog. 7:1991;237-245.
28. Michalopoulos GK, Bowen W, Nussler AN, Becich MJ, Howard TA. Comparative analysis of mitogenic and morphogenic effects of HGF and EGF on rat and human hepatocytes maintained in collagen gels. J Cell Physiol. 156:1993;443-452.
29. Rana B, Mischoulon D, Xie Y, Bucher NLR, Farmer SR. Cell-extracellular matrix interactions can regulate the switch between growth and differentiation in rat hepatocytes: reciprocal expression of C/EBPα and immediate-early growth response transcription factors. Mol Cell Biol. 14:1994;5858-5869.
30. Marceau N, Goyette R, Pelletier G, Antakly T. Hormonally-induced changes in the cytoskeleton organization of adult and newborn rat hepatocytes cultured on fibronectin precoated substratum: effect of dexamethason and insulin. Cell Mol Biol. 29:1983;421-435.
31. Sudhakaran PR, Stamatoglou SC, Hughes RC. Modulation of protein synthesis and secretion by substratum in primary cultures of rat hepatocytes. Exp Cell Res. 167:1986;505-516.
32. Schuetz EG, Li D, Omiecinski CJ, Muller-Eberhard U, Kleinman HK, Elswick B, et al. Regulation of gene expression in adult rat hepatocytes cultured on a basement membrane matrix. J Cell Physiol. 134:1988;309-323.
33. Auth MKH, Keitzer RA, Scholz M, Blaheta RA, Hottenrott EC, Herrmann G, et al. Establishment and immunological characterization of cultured human gallbladder epithelial cells. Hepatology. 18:1993;546-555.
34. Blaheta RA, Kronenberger B, Woitaschek D, Weber S, Scholz M, Schuldes H, et al. Development of an ultrasensitive in vitro assay to monitor cell growth of primary cell cultures with reduced mitotic activity. J Immunol Methods: in press.
35. Ben-Ze'ev A, Robinson GS, Bucher NLR, Farmer SR. Cell=cell and cell-matrix interactions differentially regulate the expression of hepatic and cytoskeletal genes in primary cultures of rat hepatocytes. Proc Natl Acad Sci USA. 85:1988;2161-2165.
36. Santhosh A, Sudhakaran PR. Influence of collagen gel substrata on certain biochemical activities of hepatocytes in primary culture. Mol Cell Biochem. 137:1994;127-133.
37. Baffet G, Loyer P, Glaise D, Corlu A, Etienne PL, Guguen-Guillouzo C. Distinct effects of cell-cell communication and corticosteroids on the synthesis and distribution of cytokeratins in cultured rat hepatocytes. J Cell Sci. 99:1991;609-615.
38. Arterburn LM, Zurlo J, Yager JD, Overton RM, Heifetz AH. A morphological study of differentiated hepatocytes in vitro. Hepatology. 21:1995;175-187.
39. Teti A. Regulation of cellular functions by extracellular matrix. J Am Soc Nephrol. 2:1992;83-87. (suppl.).
40. Yong K, Khwaja A. Leucocyte cellular adhesion molecules. Blood Rev. 4:1990;211-225.
41. Springer TA. Adhesion receptors of the immune system. Nature. 346:1990;425-434.
42. Chojkier M, Lyche KD, Filip M. Increased production of collagen in vivo by hepatocytes and nonparenchymal cells in rats with carbon tetrachloride-induced hepatic fibrosis. Hepatology. 8:1988;808-814.
43. Shirahase I, Ooshima A, Tanaka K, Inamoto T, Yamamoto E, Ozawa K, et al. The slow progression of hepatic fibrosis in intrahepatic cholestasis as compared with extrahepatic biliary atresia. Eur J Pediatr Surg. 5:1995;77-81.
44. Lichtinghagen R, Helmbrecht T, Arndt B, Böker KHW. Expression pattern of matrix metalloproteinases in human liver. Eur J Clin Chem Clin Biochem. 33:1995;65-71.
45. Bissell DM, Choun MO. The role of extracellular matrix in normal liver. Scand J Gastroenterol. 23:1988;1-7.
46. Carroll KM, Wong TT, Drabik DL, Chang EB. Differentiation of rat small intestinal epithelial cells by extracellular matrix. Am J Physiol. 254:1988;G355-G360.
47. Mooney D, Hansen L, Vacanti J, Langer R, Farmer S, Ingber D. Switching from differentiation to growth in hepatocytes: control by extracellular matrix. J Cell Physiol. 151:1992;497-505.
48. Sawada N, Tomomura A, Sattler CA, Sattler GL, Kleinman HK, Pitot HC. Extracellular matrix components influence DNA synthesis of rat hepatocytes in primary culture. Exp Cell Res. 167:1986;458-470.
49. Christensen L. Fibronectin, laminin, and tetranectin in human breast cancer with special attention to the extracellular matrix. APMIS. 100:1992;6-39.
50. Kato S, Otsu K, Ohtake K, Kimura Y, Yashiro T, Suzuki T, et al. Concurrent changes in sinusoidal expression of laminin and affinity of hepatocytes to laminin during rat liver regeneration. Exp Cell Res. 198:1992;59-68.
51. Martinez-Hernandez A, Delgado FM, Amenta PS. The extracellular matrix in hepatic regeneration: localization of collagen types I, III, IV, laminin, and fibronectin. Lab Invest. 64:1991;157-166.
52. Robinson J, Viti M, Höök M. Structure and properties of an under-sulfated heparan sulfate proteoglycan synthesized by a rat hepatoma cell line. J Cell Biol. 98:1984;946-953.
53. Li ML, Aggeler J, Farson DA, Hatier C, Hassell J, Bissell MJ. Influence of a reconstituted basement membrane and its components on casein gene expression and secretion in mouse mammary epithelial cells. Proc Natl Acad Sci USA. 84:1987;136-140.
54. DiPersio CM, Jackson DA, Zaret KS. The extracellular matrix coordinately modulates liver transcription factors and hepatocyte morphology. Mol Cell Biol. 11:1991;4405-4414.
55. McGuire RF, Bissell DM, Boyles J, Roll FJ. Role of extracellular matrix in regulating fenestrations of sinusoidal endothelial cells isolated from normal rat liver. Hepatology. 15:1992;989-997.
56. Clement B, Guguen-Guillouzo C, Campion JP, Glaise D, Bourel M, Guillouzo A. Long-term co-cultures of adult human hepatocytes with rat liver epithelial cells: modulation of albumin secretion and accumulation of extracellular material. Hepatology. 4:1984;373-380.