The interdomain region of dengue NS5 protein interacts with NS3 and host proteins

Dengue Bulletin

Volumn 26, Issue , 2002, Pages 155-161

  Brooks, Andrew J ^a   Johansson, Magnus ^a   Criswell, Erin ^a   Jans, David A ^b   Vasudevan, Subhash G ^a  

^a JAMES COOK UNIVERSITY   (Australia)

^b MONASH UNIVERSITY   (Australia)

Author keywords

NS3 protein; NS5 protein; Nuclear localization signal; Protein interactions

Indexed keywords

BETA GALACTOSIDASE; HELICASE; KARYOPHERIN; NUCLEAR FACTOR; PROTEINASE; RNA DIRECTED RNA POLYMERASE;

AMINO ACID SEQUENCE; BINDING SITE; CELL NUCLEUS; CONTROLLED STUDY; DENGUE; DENGUE VIRUS; FLAVIVIRUS; GENUS; NONHUMAN; NUCLEAR LOCALIZATION SIGNAL; PROTEIN DOMAIN; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION; PROTEIN TARGETING; REVIEW; TWO HYBRID SYSTEM; VIRUS CELL INTERACTION; VIRUS GENOME;

DENGUE VIRUS; FLAVIVIRUS;

EID: 0141494525     PISSN: 1020895X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Review

References (17)

1. Hare FE. The 1897 epidemic of dengue in north Queensland. The Australasian Medical Gazette, 1898, 17: 98-107.
2. Clarke T. Break-bone fever, News feature in Nature, 2002, 416: 672-674.
3. McBride WJH and Vasudevan SG. Relationship of a Dengue 2 isolate from Townsville, 1993, to international isolates. Communicable Diseases Intelligence, 1995, 19: 522-523.
4. Rice CM. 1996 Flaviviridae: The viruses and their replication p.931-960. In B.N. Fields, D.M. Knipe and P.M. Howley (ed). Fields virology. 3rd ed., Vol 1. Lippincott-Raven Publishers, Philadelphia, Pa.
5. Kapoor M, Zhang L, Ramachandra M, Kusukawa J, Ebner KE, and Padmanabhan R. Association between NS3 and NS5 proteins of dengue virus type 2 in the putative RNA replicase is linked to differential phosphorylation of NS5. J Biol Chem, 1995, 270: 19100-19106.
6. Egloff MP, Benarroch D, Selisko B, Romette JL and Canard B. An RNA cap (nucleoside-2′- O-)-methyltransferase in the flavivirus RNA polymerase NS5: crystal structure and functional characterization. EMBO, 2002, J 21: 2757-2768.
7. Koonin EV. The phylogeny of RNA-dependent RNA polymerases of positive-strand RNviruses J Gen Virol, 1991, 72:2197-2206.
8. Forwood JK, Brooks A, Briggs L J, Xiao CY, Jans DA, and Vasudevan SG. The 37-amino acid interdomain of dengue virus NS5 protein contains a functional NLS and inhibitory CK2 site. Biochem Biophys Res Commun, 1999, 257:731-737.
9. Johansson M, Brooks AJ, Jans DA and Vasudevan SG. A small region of the dengue virus-encoded RNA-dependent RNA polymerase, NS5, confers interaction with both the nuclear transport receptor importin-beta and the viral helicase, NS3. J Gen Virol, 2001, 82:735-745.
10. Brooks AJ, Johansson M, John AV, Xu Y, Jans DA, and Vasudevan SG. The interdomain region of dengue NS5 protein that binds to the viral helicase NS3 contains independently functional importin beta 1 and importin alpha/beta-reconized nuclear localization signals. J Biol Chem, 2002, 277: 36399-36407.
11. Arias CF, Preugschat F and Stauss JH. Dengue 2 virus NS2B and NS3 form a stable complex that can cleave NS3 within the helicase domain. Virology, 1993, 193:110-114.
12. Wengler G and Wengler G. The carboxy-terminal part of the NS 3 protein of the West Nile flavivirus can bde isolated as a soluble protein after proteolytic cleavage and represents an RNA-simulated NTPase. Virology, 1991, 184:707-715.
13. Buckley A, Gaidamovich S, Turchinskaya A and Gould EA. Monoclonal antibodies identify the NS5 yellow fever virus non-structural protein in the nuclei of infected cells. J Gen Virol, 1992, 73: 1125-1130.
14. Jans DA, Chan CK and Huebner S. Signals mediating nuclear targeting and their regulation: application in drug delivery. Med. Res. Reviews 18, 1998: 189-223.
15. Khromykh AA, Sedlak PI and Westaway EG. Cisand trans-acting elements in flavivirus RNA replication. J Virol, 1999, 73:9247-9255
16. Hanley KA, Lee JJ, Blaney JE., Jr. Murphy BR and Whitehead SS. Paired charge-to-alanine mutagenesis of dengue virus type 4 NS5 generates mutants with temperature-sensitive, host range, and mouse attenuation phenotypes. J Virol, 2002, 76: 525-531.
17. Catimel B, Teh T, Fontes MR, Jennings IG, Jans DA, Howlett GJ, Nice EC and Kobe B. Biophysical characterization of interactions involving importin-alpha during nuclear import J Biol Chem, 2001, 276:34189-34198.