Impedance measurements and connexin expression in human detrusor muscle from stable and unstable bladders

BJU International

Volumn 92, Issue 3, 2003, Pages 297-305

  Sui, G P ^a   Coppen, S R ^b   Dupont, E ^b   Rothery, S ^b   Gillespie, J ^d   Newgreen, D ^c   Severs, N J ^b   Fry, C H ^a,e  

^a IMPERIAL COLLEGE LONDON   (United Kingdom)

^b NATIONAL HEART AND LUNG INSTITUTE   (United Kingdom)

^c PFIZER GLOBAL RESEARCH AND DEVELOPMENT   (United Kingdom)

^d NEWCASTLE UNIVERSITY   (United Kingdom)

^e UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

Bladder instability; Connexin45; Gap junction resistance; Human bladder

Indexed keywords

CONNEXIN 40; CONNEXIN 43; CONNEXIN 45;

ALTERNATING CURRENT; ARTICLE; BLADDER INSTABILITY; CELL JUNCTION; CELLULAR DISTRIBUTION; CLINICAL ARTICLE; CONTROLLED STUDY; CYTOPLASM; DETRUSOR MUSCLE; EXTRACELLULAR SPACE; GAP JUNCTION; HUMAN; HUMAN TISSUE; IMPEDANCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION;

BLOTTING, NORTHERN; CONNEXINS; ELECTRIC CONDUCTIVITY; ELECTRIC IMPEDANCE; EXTRACELLULAR SPACE; HUMANS; IMMUNOHISTOCHEMISTRY; SARCOPLASMIC RETICULUM; URINARY BLADDER DISEASES;

EID: 0043136310     PISSN: 14644096     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1464-410X.2003.04342.x     Document Type: Article

References (32)

1. Harrison SC, Hunnam GR, Farman P, Ferguson DR, Doyle PT. Bladder instability and denervation in patients with bladder outflow obstruction. Br J Urol 1987; 60: 519-22
2. Palea S, Artibani W, Ostardo E, Trist DG, Pietra C. Evidence for purinergic neurotransmission in human urinary bladder affected by interstitial cystitis. J Urol 1993; 150: 2007-12
3. Kinder RB, Mundy AR. Pathophysiology of idiopathic detrusor instability and detrusor hyperreflexia. An in vitro study of human detrusor muscle. Br J Urol 1987; 60: 509-15
4. Elbadawi A, Yalla SV, Resnick NM. Structural basis of geriatric voiding dysfunction. III. Detrusor overactivity. J Urol 1993; 150: 1668-80
5. Elbadawi A, Yalla SV, Resnick NM. Structural basis of geriatric voiding dysfunction. IV. Bladder outlet obstruction. J Urol 1993; 150: 1681-95
6. Wu C, Bayliss M, Newgreen D, Mundy AR, Fry CH. A comparison of the mode of action of ATP and carbachol on isolated human detrusor smooth muscle. J Urol 1999; 162: 1840-7
7. Bayliss M, Wu C, Newgreen D, Mundy AR, Fry CH. A quantitative study of atropine-resistant contractions in human detrusor smooth muscle, from stable, unstable and obstructed bladders. J Urol 1999; 162: 1833-9
8. 2+ current. J Urol 2001; 165: 627-31
9. Bramich NJ, Brading AF. Electrical properties of smooth muscle in the guinea-pig urinary bladder. J Physiol 1996; 492: 185-98
10. Fry CH, Cooklin M, Birns J, Mundy AR. Measurement of intercellular electrical coupling in guinea-pig detrusor smooth muscle. J Urol 1999; 161: 660-4
11. Seki N, Karim OM, Mostwin JL. Changes in electrical properties of guinea pig smooth muscle membrane by experimental bladder outflow obstruction. Am J Physiol 1992; 262: F885-F891
12. Coppen SR, Dupont E, Rothery S, Severs NJ. Connexin45 expression is preferentially associated with the ventricular conduction system in mouse and rat heart. Circ Res 1998; 82: 232-43
13. Kilarski WM, Rothery S, Roomans GM et al. Multiple connexins localized to individual gap-junctional plaques in human myometrial smooth muscle. Microsc Res Tech 2001; 54: 114-22
14. Ko Y-S, Coppen SR, Dupont E, Rothery S, Severs NJ. Regional differentiation of desmin, connexin43 and connexin45 expression patterns in rat aortic smooth muscle. Arterioscler Thromb Vosc Biol 2001; 21: 355-64
15. Vozzi C, Dupont E, Coppen SR, Yeh H-I, Severs NJ. Chamber-related differences in connexin expression in the human heart. J Mol Cell Cardiol 1999; 31: 991-1003
16. Bruzzone R, White TW, Paul DL. Connections with connexins. The molecular basis of direct intercellular signaling. Eur J Biochem 1996; 238: 1-27
17. Cooklin M, Wallis WRJ, Sheridan DJ, Fry CH. Changes to cell-to-cell electrical coupling associated with left ventricular hypertrophy. Circ Res 1997; 80: 765-71
18. Chapman RA, Fry CH. An analysis of the cable properties of frog ventricular myocardium. J Physiol 1978; 283: 263-82
19. Schwan HP. Determination of biological impedances. In Nastuk WL ed. Techniques in Biologicol Research. Vol. VI Part B. New York, London: Academic Press, 1963
20. Naus CCG, Hearn S, Zhu D, Nicholson BJ, Shivers RR. Ultrastructural analysis of gap junctions in C6 glioma cells transfected with connexin43 cDNA. Exp Cell Res 1993; 206: 72-84
21. Severs NJ, Rothery S, Dupont E et al. Immunocytochemical analysis of connexin expression in the healthy and diseased cardiovascular system. Microsc Res Tech 2001; 52: 301-22
22. Chomezynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 1987; 162: 156-9
23. Kilarski WM, Dupont E, Coppen SR et al. Identification of two further gap-junctional proteins, connexin40 and connexin45, in human myometrial smooth muscle cells at term. Eur J Cell Biol 1998; 75: 1-8
24. Ohba M, Sakamoto Y, Tokuno H, Tomita T. Impedance components in longitudinal direction in the guinea-pig taenia coli. J Physiol 1976; 256: 527-40
25. Sui GP, Rothery S, Dupont E, Fry CH, Severs NJ. Gap junctions and connexin expression in human sub-urothelial interstitial cells. BJU Int 2002; 90: 118-29
26. Krutovskikh VA, Troyanovsky SM, Piccoli C, Tsuda H, Asamoto M, Yamasaki H. Differential effect of subcellular localization of communication impairing gap junction protein connexin43 on tumor cell growth in vivo. Oncogene 2000; 19: 505-13
27. Ko Y-S, Yeh H-I, Rothery S, Dupont E, Coppen SR, Severs NJ. Connexin make-up of endothelial gap junctions in the rat pulmonary artery as revealed by immunoconfocal microscopy and triple-label immunogold electron microscopy. J Histochem Cytochem 1999; 47: 683-91
28. Yeh H-I, Rothery S, Dupont E, Coppen SR, Severs NJ. Individual gap junction plaques contain multiple connexins in arterial endothelium. Circ Res 1998; 83: 1248-63
29. Dupont E, Ko YS, Rothery S et al. The gap-junctional protein, connexin40, is elevated in patients susceptible to post-operative atrial fibrillation. Circulation 2001; 103: 842-9
30. Gallegos CRR, Fry CH. Alterations to the electrophysiology of isolated human detrusor smooth muscle cells in bladder disease. J Urol 1994; 151: 754-8
31. Sui GP, Wu C, Fry CH. The electrophysiological properties of cultured and freshly isolated detrusor smooth muscle cells. J Urol 2001; 165: 621-6
32. Shaw RM, Rudy Y. Ionic mechanisms of propagation in cardiac tissue. Roles of the sodium and L-type calcium currents during reduced excitability and decreased gap junction coupling. Circ Res 1997; 81: 727-41