Connexin make-up of endothelial gap junctions in the rat pulmonary artery as revealed by immunoconfocal microscopy and triple-label immunogold electron microscopy

Journal of Histochemistry and Cytochemistry

Volumn 47, Issue 5, 1999, Pages 683-691

  Ko, Yu Shien ^a   Yeh, Hung I ^b   Rothery, Stephen ^a   Dupont, Emmanuel ^a   Coppen, Steven R ^a   Severs, Nicholas J ^a  

^a NATIONAL HEART AND LUNG INSTITUTE   (United Kingdom)

^b MACKAY MEMORIAL HOSPITAL   (Taiwan)

Author keywords

Confocal microscopy; Connexin; Endothelium; Gap junction; Immunogold

Indexed keywords

GAP JUNCTION PROTEIN;

ANIMAL TISSUE; ARTICLE; CELL COMMUNICATION; CONFOCAL MICROSCOPY; ELECTRON MICROSCOPY; ENDOTHELIUM CELL; GAP JUNCTION; MALE; MEMBRANE CHANNEL; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PULMONARY ARTERY; RAT; REGULATORY MECHANISM; VASCULAR ENDOTHELIUM;

ANIMALIA;

EID: 0032934609     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1177/002215549904700510     Document Type: Article

References (52)

1. Angst BD, Khan LUR, Severs NJ, Whitely K, Rothery S, Thompson RP, Magee AI, Gourdie RG (1997) Dissociated spatial patterning of gap junctions and cell adhesion junctions during postnatal differentiation of ventricular myocardium. Circ Res 80:88-94
2. Bastide B, Neyses L, Ganten D, Paul M, Willecke K, Traub O (1993) Gap junction protein connexin40 is preferentially expressed in vascular endothelium and conductive bundles of rat myocardium and is increased under hypertensive conditions. Circ Res 73:1138-1149
3. Beblo DA, Wang HZ, Beyer EC, Westphale EM, Veenstra RD (1995) Unique conductance, gating, and selective permeability properties of gap junction channels formed by connexin40. Circ Res 77:813-822
4. Brink PR, Cronin K, Banach K, Peterson E, Westphale EM, Seul KH, Ramanan SV, Beyer EC (1997) Evidence for heteromeric gap junction channels formed from rat connexin43 and human connexin37. Am J Physiol 273:C1386-1396
5. Bruzzone R, Haefliger JA, Gimlich RL, Paul DL (1993) Connexin40, a component of gap junctions in vascular endothelium, is restricted in its ability to interact with other connexins. Mol Biol Cell 4:7-19
6. Bruzzone R, White TW, Paul DL (1996) Connections with connexins: the molecular basis of direct intercellular signaling. Eur J Biochem 238:1-27
7. Bukauskas FF, Elfgang C, Willecke K, Weingart R (1995) Biophysical properties of gap junction channels formed by mouse connexin40 in induced pairs of transfected human HeLa cells. Biophys J 68:2289-2298
8. Cao F, Eckert R, Elfgang C, Nitsche JM, Snyder SA, Hülser DF, Willecke K, Nicholson BJ (1998) A quantitative analysis of connexin-specific permeability differences of gap junctions expressed in HeLa transfectants and Xenopus oocytes. J Cell Sci 111:31-43
9. 3. J Cell Sci 109:1765-1773
10. Christ GJ, Spray DC, el-Sabban M, Moore LK, Brink PR (1996) Gap junctions in vascular tissues - evaluating the role of intercellular communication in the modulation of vasomotor tone. Circ Res 79:631-646
11. Coppen SR, Dupont E, Rothery S, Severs NJ (1998) Connexin45 expression is preferentially associated with the ventricular conduction system in mouse and rat heart. Circ Res 82:232-243
12. Cowan DB, Lye SJ, Langille BL (1998) Regulation of vascular connexin43 gene expression by mechanical loads. Circ Res 82: 786-793
13. Diez JA, Ahmad S, Evans WH (1998) Biogenesis of liver gap junctions. In Werner R, ed. Gap Junctions. Amsterdam, IOS Press, 130-134
14. Elfgang C, Eckert R, Lichtenberg-Fraté H, Butterweck A, Traub O, Klein RA, Hülser DF, Willecke K (1995) Specific permeability and selective formation of gap junction channels in connexin-transfected HeLa cells. J Cell Biol 129:805-817
15. Gabriels JE, Paul DL (1998) Connexin43 is highly localized to sites of disturbed flow in rat aortic endothelium but connexin37 and connexin40 are more uniformly distributed. Circ Res 83:636-643
16. Haubrich S, Schwarz H-J, Bukauskas F, Lichtenberg-Fraté H, Traub O, Weingart R, Willecke K (1996) Incompatibility of connexin 40 and 43 hemichannels in gap junctions between mammalian cells is determined by intracellular domains. Mol Biol Cell 7:1995-2006
17. Huttner I, Walker C, Gabbiani G (1985) Aortic endothelial cell during regeneration. Remodeling of cell junctions, stress fibers, and stress fiber-membrane attachment domains. Lab Invest 53:287-302
18. Inoguchi T, Ueda F, Umeda F, Yamashita T, Nawata H (1995) Inhibition of intercellular communication via gap junction in cultured aortic endothelial cells by elevated glucose and phorbol ester. Biochem Biophys Res Commun 208:492-497
19. Jiang WG, Bryce RP, Mansel RE (1997) Gamma linolenic acid regulates gap junction communication in endothelial cells and their interaction with tumour cells. Prostaglandins Leukotrienes Essent Fatty Acids 56:307-316
20. Jiang JX, Goodenough DA (1996) Heteromeric connexons in lens gap junction channels. Proc Natl Acad Sci USA 93:1287-1291
21. Kitamura Y, Morita I, Nihei Z, Mishima Y, Murota S (1997) Effect of IL-6 on tumor cell invasion of vascular endothelial monolayers. Surg Today 27:534-541
22. Ko Y-S, Yeh H-I, Rothery S, Dupont E, Severs NJ (1998) Simultaneous localization of connexins 40, 37 and 43 in pulmonary artery endothelial gap junctions. In Werner R, ed. Gap Junctions. Amsterdam, ISO Publishers, 183-187
23. Koval M, Geist ST, Westphale EM, Kemendy AE, Civitelli R, Beyer EC, Steinberg TH (1995) Transfected connexin45 alters gap junction permeability in cells expressing endogenous connexin43. J Cell Biol 130:987-995
24. Kumar NM, Gilula NB (1996) The gap junction communication channel. Cell 84:381-388
25. Larson DM, Haudenschild CC (1988) Junctional transfer in wounded cultures of bovine aortic endothelial cells. Lab Invest 59:373-379
26. Larson DM, Haudenschild CC, Beyer EC (1990) Gap junction messenger RNA expression by vascular wall cells. Circ Res 66:1074-1080
27. Larson DM, Sheridan JD (1982) Intercellular junctions and transfer of small molecules in primary vascular endothelial cultures. J Cell Biol 92:183-189
28. Larson DM, Sheridan JD (1985) Junctional transfer in cultured vascular endothelium. II. Dye nucleotide transfer. J Membr Biol 83: 157-167
29. Larson DM, Wrobleski MJ, Sagar GDV, Westphale EM, Beyer EC (1997) Differential regulation of connexin43 and connexin37 in endothelial cells by cell density, growth, and TGF-b1. Am J Physiol 272:C405-415
30. Little TL, Beyer EC, Duling BR (1995) Connexin 43 and connexin 40 gap junctional proteins are present in arteriolar smooth muscle and endothelium in vivo. Am J Physiol 268:H729-739
31. Mazzoleni G, Camplani A, Telo P, Pozzi A, Tanganelli S, Elfgang C, Willecke K, Ragnotti G (1996) Effect of tumor-promoting and anti-promoting chemicals on the viability and junctional coupling of human HeLa cells transfected with DNAs coding for various murine connexin proteins. Comp Biochem Physiol [C] 113:247-256
32. Moreno AP, Rook MB, Fishman GI, Spray DC (1994) Gap junction channels: Distinct voltage-sensitive and -insensitive conductance states. Biophys J 67:113-119
33. Paul DL, Ebihara L, Takemoto LJ, Swenson KI, Goodenough DA (1991) Connexin46, a novel lens gap junction protein, induces voltage-gated currents in nonjunctional plasma membrane of Xenopus oocytes. J Cell Biol 115:1077-1089
34. Pepper MS, Meda P (1992) Basic fibroblast growth factor increases junctional communication and connexin 43 expression in microvascular endothelial cells. J Cell Physiol 153:196-205
35. Pepper MS, Montesano R, El Aoumari A, Gros D, Orci L, Meda P (1992) Coupling and connexin 43 expression in microvascular and large vessel endothelial cells. Am J Physiol 262:C1246-1257
36. Pepper MS, Spray DC, Chanson MC, Montesano R, Orci L, Meda P (1989) Junctional communication is induced in migrating capillary endothelial cells. J Cell Biol 109:3027-3038
37. Reed KE, Westphale EM, Larson DM, Wang H-Z, Veenstra RD, Beyer EC (1993) Molecular cloning and functional expression of human connexin37, an endothelial cell gap junction protein. J Clin Invest 91:997-1004
38. Severs NJ, Dupont E, Kaprielian RR, Yeh H-I, Rothery S (1996) Gap junctions and connexins in the cardiovascular system. In Yacoub MH, Carpentier A, Pepper J, Fabiani J-N, eds. Annual of Cardiac Surgery 1996. 9th ed. London, Current Science, 31-44
39. Spagnoli LG, Pietra GG, Villaschi S, Johns LW (1982) Morphometric analysis of gap junctions in regenerating arterial endothelium. Lab Invest 46:139-148
40. 1-connexin (connexin-32) and b2-connexin (connexin-26) can form heteromeric hemichannels. J Biol Chem 270:6768-6772
41. Stevenson S, Rothery S, Cullen MJ, Severs NJ (1998) Spatial relationship of C-terminal domains of dystrophin and beta-dystroglycan in cardiac muscle support a direct molecular interaction at the plasma membrane interface. Circ Res 82:82-93
42. Traub O, Eckert R, Lichtenberg-Fraté H, Elfgang C, Bastide B, Scheidtmann KH, Hülser DF, Willecke K (1994) Immunochemical and electrophysiological characterization of murine connexin40 and -43 in mouse tissues and transfected human cells. Eur J Cell Biol 64:101-112
43. van Rijen HVM, van Kempen MJA, Analbers LJS, Rook MB, van Ginneken ACG, Gros D, Jongsma HJ (1997) Gap junctions in human umbilical cord endothelial cells contain multiple connexins. Am J Physiol 272:C117-130
44. van Rijen HV, van Kempen MJ, Postma S, Jongsma HJ (1998) Tumour necrosis factor alpha alters the expression of connexin43, connexin40, and connexin37 in human umbilical vein endothelial cells. Cytokines 10:258-264
45. Veenstra RD (1996) Size and selectivity of gap junction channels formed from different connexins. J Bioenerg Biomembr 28:327-337
46. Veenstra RD, Wang H-Z, Beyer EC, Ramanan SV, Brink PR (1994) Connexin37 forms high conductance gap junction channels with subconductance state activity and selective dye and ionic permeabilities. Biophys J 66:1915-1928
47. Wolosin JM, Schütte M, Chen SH (1997) Connexin distribution in the rabbit and rat ciliary body - a case for heterotypic epithelial gap junctions. Invest Ophthalmol Vis Sci 38:341-348
48. Xie H, Hu VW (1994) Modulation of gap junctions in senescent endothelial cells. Exp Cell Res 214:172-176
49. Xie HQ, Laird DW, Chang TH, Hu VW (1997) A mitosis-specific phosphorylation of the gap junction protein connexin43 in human vascular cells: biochemical characterization and localization. J Cell Biol 137:203-210
50. Yeh H-I (1997) Vascular cell gap junctions: diversity of connexin expression in the healthy and injured artery. PhD Thesis, University of London
51. Yeh H-I, Dupont E, Coppen S, Rothery S, Severs NJ (1997) Gap junction localization and connexin expression in cytochemically identified endothelial cells from arterial tissue. J Histochem Cytochem 45:539-550
52. Yeh H-I, Rothery S, Dupont E, Coppen SR, Severs NJ (1998) Individual gap junction plaques contain multiple connexins in arterial endothelium. Circ Res 83:1248-1263