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Volumn 42, Issue 32, 2003, Pages 3766-3771

Single-molecule covalent chemistry with spatially separated reactants

Author keywords

Kinetics; Nanoreactors; Protein pores; Single molecule studies; Thiols

Indexed keywords

DIFFUSION; ELECTRIC CURRENTS; PORE SIZE; PROTEINS;

EID: 0042865987     PISSN: 14337851     EISSN: None     Source Type: Journal    
DOI: 10.1002/anie.200351313     Document Type: Article
Times cited : (104)

References (33)
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    • note
    • DTT is the volume of solution occupied by one DTT molecule, based on the external concentration).
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    • note
    • At high concentrations of DTT(cis), the reduction in rate most likely arises from depletion of DTNB(trans) by reaction with DTT that has reached the trans unstirred layer.
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    • note
    • To check whether the intermediate 2 was indeed formed from DTT, we carried out several controls. For example, in the absence of DTT, the reaction proceeded through the first step, but remained there indefinitely (> 60 s; Figure 2 a, upper trace). Further, when DTT was replaced with 50 μM β-mercaptoethanol (βME, cis), a monothiol, the reaction took a different course. The first step with DTNB (50 μM, trans) was observed as before, but it was followed by a step to a level where the system remained for a prolonged period (>60s), which was distinct from the level representing 2. We interpret the sequence as cleavage of 1 with βME to generate a mixed disulfide that reacts relatively slowly with a second molecule of βME.
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    • note
    • 2+ ions are picked up, and oxidized on the other side, where they are released.
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* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.