Real-time PCR for simultaneous detection and quantification of Borrelia burgdorferi in field-collected Ixodes scapularis ticks from the Northeastern United States

Applied and Environmental Microbiology

Volumn 69, Issue 8, 2003, Pages 4561-4565

  Wang, Guiqing ^a   Liveris, Dionysios ^a   Brei, Brandon ^a   Wu, Hongyan ^a   Falco, Richard C ^b   Fish, Durland ^a   Schwartz, Ira ^a,c  

^a NEW YORK MEDICAL COLLEGE   (United States)

^b YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c FORDHAM UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AGGLOMERATION; ECOLOGY;

POLYMORPHISM;

MICROBIOLOGY;

BACTERIAL DNA; RNA 16S; RNA 23S;

POLYMERASE CHAIN REACTION;

ARTICLE; BACTERIUM ISOLATE; BORRELIA BURGDORFERI; IXODES SCAPULARIS; NONHUMAN; PARASITE VECTOR; POLYMERASE CHAIN REACTION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; UNITED STATES;

ANIMALS; BORRELIA BURGDORFERI; COLONY COUNT, MICROBIAL; GENOTYPE; IXODES; POLYMERASE CHAIN REACTION;

ACARI; ARACHNIDA; BACTERIA (MICROORGANISMS); BORRELIA; BORRELIA BURGDORFERI; HEXAPODA; IXODES; IXODES SCAPULARIS; SPIROCHAETALES;

EID: 0042530324     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.69.8.4561-4565.2003     Document Type: Article

References (39)

1. Brunet, L. R., A. Spielman, and S. R. Telford. 1995. Density of Lyme disease spirochetes within deer ticks collected from zoonotic sites. Am. J. Trop. Med. Hyg. 53:300-302.
2. Burgdorfer, W., J. F. Anderson, L. Gern, R. S. Lane, J. Piesman, and A. Spielman. 1991. Relationship of Borrelia burgdorferi to its arthropod vectors. Scand. J. Infect. Dis. Suppl. 77:35-40.
3. Burkot, T. R., L. Patrican, and J. Piesman. 1994. Field trial of an outer surface protein A (OspA) antigen-capture enzyme-linked immunosorbent assay (ELISA) to detect Borrelia burgdorferi in Ixodes scapularis. Am. J. Trop. Med. Hyg. 50:354-358.
4. Burkot, T. R., J. Piesman, and R. A. Wirtz. 1994. Quantitation of the Borrelia burgdorferi outer surface protein A in Ixodes scapularis: fluctuations during the tick life cycle, doubling times, and loss while feeding. J. Infect. Dis. 170:883-889.
5. Burkot, T. R., R. A. Wirtz, B. Luft, and J. Piesman. 1993. An OspA antigen-capture enzyme-linked immunosorbent assay for detecting North American isolates of Borrelia burgdorferi in larval and nymphal Ixodes dammini. J. Clin. Microbiol. 31:272-278.
6. Daniels, T. J., T. M. Boccia, S. Varde, J. Marcus, J. Le, D. J. Bucher, R. C. Falco, and I. Schwartz. 1998. Geographic risk for Lyme disease and human granulocytic ehrlichiosis in southern New York State. Appl. Environ. Microbiol. 64:4663-4669.
7. Daniels, T. J., and D. Fish. 1990. Spatial distribution and dispersal of unfed larval Ixodes dammini (Acari: Ixodidae) in southern New York, USA. Environ. Entomol. 19:1029-1033.
8. de Silva, A. M., and E. Fikrig. 1995. Growth and migration of Borrelia burgdorferi in Ixodes ticks during blood feeding. Am. J. Trop. Med. Hyg. 53:397-404.
9. de Silva, A. M., N. S. Zeidner, Y. Zhang, M. C. Dolan, J. Piesman, and E. Fikrig. 1999. Influence of outer surface protein A antibody on Borrelia burgdorferi within feeding ticks. Infect. Immun. 67:30-35.
10. Fraser, C. M., S. Casjens, W. M. Huang, G. G. Sutton, R. Clayton, R. Lathigra, O. White, K. A. Ketchum, R. Dodson, E. K. Hickey, M. Gwinn, B. Dougherty, J. F. Tomb, R. D. Fleischmann, D. Richardson, J. Peterson, A. R. Kerlavage, J. Quackenbush, S. Salzberg, M. Hanson, R. van Vugt, N. Palmer, M. D. Adams, J. Gocayne, and J. C. Venter. 1997. Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi. Nature 390:580-586.
11. Gern, L, N. Lebet, and J. Moret. 1996. Dynamics of Borrelia burgdorferi infection in nymphal Ixodes ricinus ticks during feeding. Exp. Appl. Acarol. 20:649-658.
12. Gregory, R. D., and M. E. Woolhouse. 1993. Quantification of parasite aggregation: a simulation study. Acta Trop. 54:131-139.
13. Iyer, R., D. Liveris, A. Adams, J. Nowakowski, D. McKenna, S. Bittker, D. Cooper, G. P. Wormser, and I. Schwartz. 2001. Characterization of Borrelia burgdorferi isolated from erythema migrans lesions: interrelationship of three molecular typing methods. J. Clin. Microbiol. 39:2954-2957.
14. Lebet, N., and L. Gern. 1994. Histological examination of Borrelia burgdorferi infections in unfed Ixodes ricinus nymphs. Exp. Appl. Acarol. 18:177-183.
15. Leutenegger, C. M., N. Pusterla, C. N. Mislin, R. Weber, and H. Lutz. 1999. Molecular evidence of coinfection of ticks with Borrelia burgdorferi sensu lato and the human granulocytic ehrlichiosis agent in Switzerland. J. Clin. Microbiol. 37:3390-3391.
16. Levin, M. L., and D. Fish. 2000. Acquisition of coinfection and simultaneous transmission of Borrelia burgdorferi and Ehrlichia phagocytophila by Ixodes scapularis ticks. Infect. Immun. 68:2183-2186.
17. Liveris, D., A. Gazumyan, and I. Schwartz. 1995. Molecular typing of Borrelia burgdorferi sensu lato by PCR-restriction fragment length polymorphism analysis. J. Clin. Microbiol. 33:589-595.
18. Liveris, D., S. Varde, R. Iyer, S. Koenig, S. Bittker, D. Cooper, D. McKenna, J. Nowakowski, R. B. Nadelman, G. P. Wormser, and I. Schwartz. 1999. Genetic diversity of Borrelia burgdorferi in Lyme disease patients as determined by culture versus direct PCR with clinical specimens. J. Clin. Microbiol. 37:565-569.
19. Liveris, D., G. Wang, G. Girao, D. W. Byrne, J. Nowakowski, D. McKenna, R. Nadelman, G. P. Wormser, and I. Schwartz. 2002. Quantitative detection of Borrelia burgdorferi in 2-millimeter skin samples of erythema migrans lesions: correlation of results with clinical and laboratory findings. J. Clin. Microbiol. 40:1249-1253.
20. Lord, C. C., B. Barnard, K. Day, J. W. Hargrove, J. J. McNamara, R. E. Paul, K. Trenholme, and M. E. Woolhouse. 1999. Aggregation and distribution of strains in microparasites. Philos. Trans. R. Soc. Lond. B 354:799-807.
21. Ma, Y., K. P. Seiler, E. J. Eichwald, J. H. Weis, C. Teuscher, and J. J. Weis. 1998. Distinct characteristics of resistance to Borrelia burgdorferi-induced arthritis in C57BL/6N mice. Infect. Immun. 66:161-168.
22. Mommert, S., R. Gutzmer, A. Kapp, and T. Werfel. 2001. Sensitive detection of Borrelia burgdorferi sensu lato DNA and differentiation of Borrelia species by LightCycler PCR. J. Clin. Microbiol. 39:2663-2667.
23. Morrison, T. B., Y. Ma, J. H. Weis, and J. J. Weis. 1999. Rapid and sensitive quantification of Borrelia burgdorferi-infected mouse tissues by continuous fluorescent monitoring of PCR. J. Clin. Microbiol. 37:987-992.
24. Pahl, A., U. Kuhlbrandt, K. Brune, M. Rollinghoff, and A. Gessner. 1999. Quantitative detection of Borrelia burgdorferi by real-time PCR. J. Clin. Microbiol. 37:1958-1963.
25. Parola, P., and D. Raoult. 2001. Ticks and tickborne bacterial diseases in humans: an emerging infectious threat. Clin. Infect. Dis. 32:897-928.
26. Pichon, G., H. P. Awono-Ambene, and V. Robert. 2000. High heterogeneity in the number of Plasmodium falciparum gametocytes in the bloodmeal of mosquitoes fed on the same host. Parasitology 121:115-120.
27. Piesman, J., J. R. Oliver, and R. J. Sinsky. 1990. Growth kinetics of the Lyme disease spirochete (Borrelia burgdorferi) in vector ticks (Ixodes dammini). Am. J. Trop. Med. Hyg. 42:352-357.
28. Pietila, J., Q. He, J. Oksi, and M. K. Viljanen. 2000. Rapid differentiation of Borrelia garinii from Borrelia afzelii and Borrelia burgdorferi sensu stricto by Lightcycler fluorescence melting curve analysis of a PCR product of the recA gene. J. Clin. Microbiol. 38:2756-2759.
29. Randolph, S. E., D. Miklisova, J. Lysy, D. J. Rogers, and M. Labuda. 1999. Incidence from coincidence: patterns of tick infestations on rodents facilitate transmission of tick-borne encephalitis virus. Parasitology 118:177-186.
30. Rauter, C., R. Oehme, I. Diterich, M. Engele, and T. Hartung. 2002. Distribution of clinically relevant Borrelia genospecies in ticks assessed by a novel, single-run, real-time PCR. J. Clin. Microbiol. 40:36-43.
31. Schwartz, I., S. Varde, R. B. Nadelman, G. P. Wormser, and D. Fish. 1997. Inhibition of efficient polymerase chain reaction amplification of Borrelia burgdorferi DNA in blood-fed ticks. Am. J. Trop. Med. Hyg. 56:339-342.
32. Scoles, G. A., M. Papero, L. Beati, and D. Fish. 2001. A relapsing fever group spirochete transmitted by Ixodes scapularis ticks. Vector Borne Zoonotic Dis. 1:21-34.
33. Shaw, D. J., B. T. Grenfell, and A. P. Dobson. 1998. Patterns of macroparasite aggregation in wildlife host populations. Parasitology 117:597-610.
34. Stafford, K. C., M. L. Cartter, L. A. Magnarelli, S. H. Ertel, and P. A. Mshar. 1998. Temporal correlations between tick abundance and prevalence of ticks infected with Borrelia burgdorferi and increasing incidence of Lyme disease. J. Clin. Microbiol. 36:1240-1244.
35. Straubinger, R. K. 2000. PCR-based quantification of Borrelia burgdorferi organisms in canine tissues over a 500-day postinfection period. J. Clin. Microbiol. 38:2191-2199.
36. Wang, G., C. Ojaimi, R. Iyer, V. Saksenberg, S. A. McClain, G. P. Wormser, and I. Schwartz. 2001. Impact of genotypic variation of Borrelia burgdorferi sensu stricto on kinetics of dissemination and severity of disease in C3H/HeJ mice. Infect. Immun. 69:4303-4312.
37. Wang, G., C. Ojaimi, H. Wu, V. Saksenberg, R. Iyer, D. Liveris, S. A. McClain, G. P. Wormser, and I. Schwartz. 2002. Disease severity in a murine model of Lyme borreliosis is associated with the genotype of the infecting Borrelia burgdorferi sensu stricto strain. J. Infect. Dis. 186:782-791.
38. Wittwer, C. T., K. M. Ririe, R. V. Andrew, D. A. David, R. A. Gundry, and U. J. Balis. 1997. The LightCycler: a microvolume multisample fluorimeter with rapid temperature control. BioTechniques 22:176-181.
39. Woolhouse, M. E., C. Dye, J. F. Etard, T. Smith, J. D. Charlwood, G. P. Garnett, P. Hagan, J. L. Hii, P. D. Ndhlovu, R. J. Quinnell, C. H. Watts, S. K. Chandiwana, and R. M. Anderson. 1997. Heterogeneities in the transmission of infectious agents: implications for the design of control programs. Proc. Natl. Acad. Sci. USA 94:338-342.