Genetic diversity of Borrelia burgdorferi in lyme disease patients as determined by culture versus direct PCR with clinical specimens

Journal of Clinical Microbiology

Volumn 37, Issue 3, 1999, Pages 565-569

  Liveris, Dionysios ^a   Varde, Shobha ^a   Iyer, Radha ^a   Koenig, Seth ^a   Bittker, Susan ^a   Cooper, Denise ^a   McKenna, Donna ^a   Nowakowski, John ^a   Nadelman, Robert B ^a   Wormser, Gary P ^a   Schwartz, Ira ^a  

^a NEW YORK MEDICAL COLLEGE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

RIBOSOME DNA;

ARTICLE; BACTERIUM CULTURE; BACTERIUM ISOLATE; BLOOD ANALYSIS; BORRELIA BURGDORFERI; CONTROLLED STUDY; ERYTHEMA CHRONICUM MIGRANS; GENETIC VARIABILITY; HUMAN; HUMAN TISSUE; INTERMETHOD COMPARISON; LYME DISEASE; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; SKIN BIOPSY;

BACTERIA (MICROORGANISMS); BORRELIA; BORRELIA BURGDORFERI;

EID: 0032998899     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.37.3.565-569.1999     Document Type: Article

References (33)

1. Assous, M. V., D. Postic, G. Paul, P. Névot, and G. Baranton. 1993. Western blot analysis of sera from Lyme borreliosis patients according to the genomic species of the Borrelia strains used as antigens. Eur. J. Clin. Microbiol. Infect. Dis. 12:261-268.
2. Balmelli, T., and J. C. Piffaretti. 1995. Association between different clinical manifestations of Lyme disease and different species of Borrelia burgdorferi sensu lato. Res. Microbiol. 146:329-340.
3. Baranton, G., D. Postic, I. Saint Girons, P. Boerlin, J.-C. Piffaretti, M. Assous, and P. A. D. Grimont. 1992. Delineation of Borrelia burgdorferi sensu stricto, Borrelia garinii sp. nov., and group VS461 associated with Lyme borreliosis. Int. J. Syst. Bacteriol. 42:378-383.
4. Barbour, A. G., and D. Fish. 1993. The biological and social phenomenon of Lyme disease. Science 260:1610-1616.
5. Benach, J. L., E. M. Bosler, J. P. Hanrahan, J. L. Coleman, G. S. Habicht, T. F. Bast, D. J. Cameron, J. L. Ziegler, A. G. Barbour, W. Burgdorfer, R. Edelman, and R. A. Kaslow. 1983. Spirochetes isolated from the blond of two patients with Lyme disease. N. Engl. J. Med. 308:740-742.
6. Canica, M. M., F. Nato, L. du Merle, J. C. Mazie, G. Baranton, and D. Postic. 1993. Monoclonal antibodies for identification of Borrelia afzelii sp. nov. associated with late cutaneous manifestations of Lyme borreliosis. Scand. J. Infect. Dis. 25:441-448.
7. Demarschalck, I., A. B. Messaoud, M. De Kesel, B. Hoyois, Y. Lobet, P. Hoet, G. Bigaignon, A. Bollen, and E. Godfroid. 1995. Simultaneous presence of different Borrelia burgdorferi genospecies in biological fluids of Lyme disease patients. J. Clin. Microbiol. 33:602-608.
8. Foretz, M., D. Postic, and G. Baranton. 1997. Phylogenetic analysis of Burrelia burgdorferi sensu stricto by arbitrarily primed PCR and pulsed-field gel electrophoresis. Int. J. Syst. Bacteriol. 47:11-18.
9. Fraser, C. M., S. Casjens, W. Huang, G. G. Sutton, R. Clayton, R. Lathigra, O. White, K. A. Ketchum, R. Dodson, E. K. Hickey, M. Gwinn, B. Dougherty, J.-F. Tomb, R. D. Fleischmann, D. Richardson, J. Peterson, A. R. Kerlavage, J. Quackenbush, S. Salzberg, M. Hanson, R. van Vugt, N. Palmer, M. D. Adams, J. Gocayne, J. Weidman, T. Utterback, L. Watthey, L. McDonald, P. Artiach, C. Bowman, S. Garland, C. Fujii, M. D. Cotton, K. Horst, K. Roberts, B. Hatch, H. O. Smith, and J. C. Venter. 1997. Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi. Nature 390:580-586.
10. Gazumyan, A., J. J. Schwartz, D. Liveris, and I. Schwartz. 1994. Sequence analysis of the ribosomal RNA operon of the Lyme disease spirochete, Borrelia burgdorferi. Gene 146:57-65.
11. Guttman, D. S., P. W. Wang, I. N. Wang, E. M. Bosler, B. J. Luft, and D. E. Dykhuizen. 1996. Multiple infections of Ixodes scapularis ticks by Borrelia burgdorferi as revealed by a single-strand conformation polymorphism analysis. J. Clin. Microbiol. 34:652-656.
12. Iyer, R., D. Liveris, A. Adams, and I. Schwartz. Unpublished data.
13. Kawabata, H., T. Masuzawa, and Y. Yanagihara. 1993. Genomic analysis of Borrelia japonica sp. nov. isolated from Ixodes ovatus in Japan. Microbiol. Immunol. 37:843-848.
14. Le Flech, A., D. Postic, K. Girardet, O. Peter, and G. Baranton. 1997. Characterization of Borrelia lusitaniae sp. nov. by 16S ribosomal DNA sequence analysis. Int. J. Syst. Bacteriol. 47:921-925.
15. Liveris, D., A. Gazumyan, and I. Schwartz. 1995. Molecular typing of Borrelia burgdorferi sensu lato by PCR-restriction fragment length polymorphism analysis. J. Clin. Microbiol. 33:589-595.
16. Liveris, D., and I. Schwartz. Unpublished data.
17. Liveris, D., G. P. Wormser, J. Nowakowski, R. Nadelman, S. Bittker, D. Cooper, S. Varde, F. H. Moy, G. Forseter, C. S. Pavia, and I. Schwartz. 1996. Molecular typing of Borrelia burgdorferi from Lyme disease patients by PCR-restriction fragment length polymorphism analysis. J. Clin. Microbiol. 34: 1306-1309.
18. Marconi, R. T., D. Liveris, and I. Schwartz. 1995. Identification of novel insertion elements, restriction fragment length polymorphism patterns, and discontinuous 23S rRNA in Lyme disease spirochetes: phylogenetic analyses of rRNA genes and their intergenic spacers in Borrelia japonica sp. nov. and genomic group 21038 (Borrelia andersonii sp. nov.) isolates. J. Clin. Microbiol. 33:2427-2434.
19. Mathiesen, D. A., J. H. Oliver, Jr., C. P. Kolbert, E. D. Tullson, B. J. Johnson, G. L. Campbell, P. D. Mitchell, K. D. Reed, S. R. Telford 3rd, J. F. Anderson, R. S. Lane, and D. H. Persing. 1997. Genetic heterogeneity of Borrelia burgdorferi in the United States. J. Infect. Dis. 175:98-107.
20. Nadelman, R. B., J. Nowakowski, G. Forseter, N. S. Goldberg, S. Bittker, D. Cooper, M. Aguero-Rosenfeld, and G. P. Wormser. 1996. The clinical spectrum of early Lyme borreliosis in patients with culture-confirmed erythema migrans. Am. J. Med. 100:502-508.
21. Nadelman, R., C. Pavia, L. Magnarelli, and G. Wormser. 1990. Isolation of Borrelia burgdorferi from the blood of seven patients with Lyme disease. Am. J. Med. 88:21-26.
22. Norris, D. E., B. J. B. Johnson, J. Piesman, G. O. Maupin, J. L. Clark, and W. C. Black IV. 1997. Culturing selects for specific genotypes of Borrelia burgdorferi in an enzootic cycle in Colorado. J. Clin. Microbiol. 35:2359-2364.
23. Pichon, B., E. Godfroid, B. Hoyois, A. Bollen, F. Rodhain, and C. Perez-Eid. 1995. Simultaneous infection of Ixodes ricinus nymphs by two Borrelia burgdorferi sensu lato species: possible implications for clinical manifestations. Emerg. Infect. Dis. 1:89-90.
24. Schwartz, I., S. Varde, R. B. Nadelman, G. P. Wormser, and D. Fish. 1997. Inhibition of efficient polymerase chain reaction amplification of DNA in blood-fed ticks. Am. J. Trop. Med. Hyg. 56:339-342.
25. Schwartz, I., G. P. Wormser, J. J. Schwartz, D. Cooper, P. Weissensee, A. Gazumyan, E. Zimmermann, N. S. Goldberg, S. Bittker, G. L. Campbell, and C. S. Pavia. 1992. Diagnosis of early Lyme disease by polymerase chain reaction amplification and culture of skin biopsies from erythema migrans lesions. J. Clin. Microbiol. 30:3082-3088.
26. Spach, D. H., W. C. Liles, G. L. Campbell, R. E. Quick, D. E. Anderson, and T. R. Fritsche. 1993. Tick-borne diseases in the United States, N. Engl. J. Med. 329:936-947.
27. Steere, A. C., R. L. Grodzicki, A. N. Kornblatt, J. E. Craft, A. G. Barbour, W. Burgdorfer, G. P. Schmid, E. Johnson, and S. E. Malwista. 1983. The spirochetal etiology of Lyme disease. N. Engl. J. Med. 308:733-740.
28. Strle, F., R. N. Ricken, Y. Cheng, J. Cimperman, V. Maraspin, S. Lotric-Furlan, E. Ruzic-Sabljic, and M. M. Picken. 1997. Clinical findings for patients with Lyme boneliosis caused by Borrelia burgdorferi sensu lato with genotypic and phenotypic similarities to strain 25015. Clin. Infect. Dis. 25: 273-280.
29. van Dam, A. P., H. Kuiper, K. Vos, A. Widjojokusumo, B. M. de Jongh, L. Spanjaard, A. C. P. Ramselaar, M. D. Kramer, and J. Dankert. 1993. Different genospecies of Borrelia burgdorferi are associated with distinct clinical manifestations of Lyme borreliosis. Clin. Infect. Dis. 17:708-717.
30. Walker, D. H., A. G. Barbour, J. H. Oliver, R. S. Lane, J. S. Dumler, D. T. Dennis, D. H. Persing, A. F. Azad, and E. McSweegan. 1996. Emerging bacterial zoonotic and vector-borne diseases. Ecological and epidemiological factors. JAMA 275:463-469.
31. Welsh, J., C. Pretzman, D. Postic, I. Girons, G. Baranton, and M. McClelland. 1992. Genomic fingerprinting by arbitrarily primed polymerase chain reaction resolves Borrelia burgdorferi into three distinct phyletic groups. Int. J. Syst. Bacteriol. 42:370-377.
32. Wilske, B., V. Preac-Mursic, U. B. Göbel, B. Graf, S. Jauris, E. Soutschek, E. Schwab, and G. Zumstein. 1993. An OspA serotyping system for Borrelia burgdorferi based on reactivity with monoclonal antibodies and OspA sequence analysis. J. Clin. Microbiol. 31:340-350.
33. Wormser, G. P., J. Nowakowski, R. B. Nadelman, S. Bittker, D. Cooper, and C. Pavia. 1998. Improving the yield of blood cultures for patients with early Lyme disease. J. Clin. Microbiol. 36:296-298.