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note
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6 bacteria were injected, and after 3 to 4 days ∼109 bacteria were recovered, resulting in 10 generations of growth.
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0342849253
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note
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The sequences of the relevant genes were determined as previously described (4, 6). For each of the FusR-compensated mutants, the fusA gene was sequenced, and for each of the SmR-compensated mutants, the sequences of the rpsD, rpsE, and rpsL genes were determined. To confirm that the compensatory mutations identified by sequencing conferred compensation, they were transferred to a new genetic background by transduction using linked Tn10 elements.
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0343283950
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note
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Single-letter abbreviations for the amino acid residues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; C, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and V. Tyr.
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Supplementary data is available at Science Online at www.sciencemag.org/feature/data/1045726.shl.
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Science
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note
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-7 in the spleen.
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0342849246
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note
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gen (wt) ln(A/B) + tln2], where A is the number of mutant bacteria and B is the number of wild-type bacteria. The relative fitness was calculated as the ratio of the generation time of the wild type divided by the generation time of each individual mutant. The relative fitness of the wild type in mice was set to 1. The generation time of the wild type in LB was 26 min and its relative fitness was set to 1.
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31
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0342849244
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note
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Supported by the Swedish Medical and Natural Sciences Research Councils, the Swedish Institute for Infectious Disease Control, Lovens Pharmaceutical, and the European Union Biotechnology Research Program.
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