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Volumn 272, Issue 5268, 1996, Pages 1641-1643

Compensatory ahpC gene expression in isoniazid-resistant Mycobacterium tuberculosis

Author keywords

[No Author keywords available]

Indexed keywords

CATALASE; ISONIAZID; PEROXIDASE; PEROXIDE;

EID: 0029996734     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.272.5268.1641     Document Type: Article
Times cited : (384)

References (24)
  • 15
    • 15844372116 scopus 로고    scopus 로고
    • note
    • A 675-bp sequence upstream of the katG gene of MTB strain Erdman was used to drive expression of the lux gene in an integrating shuttle vector. Fidelity of constructs was determined by sequence analysis. Transformations into BCG and lux assays were done as described (23).
  • 16
    • 15844402874 scopus 로고    scopus 로고
    • personal communication
    • S. T. Cole and B. Heym, personal communication.
    • Cole, S.T.1    Heym, B.2
  • 17
    • 15844403258 scopus 로고    scopus 로고
    • note
    • Mid-log H37Rv were diluted and spread on 7H11 plates with INH (0, 0.5, 1.0, and 10 μg/ml). INH-resistant single colony isolates were re-streaked at each level on fresh plates with the same amount of INH, and then bacteria were collected and lysed as described (6). Lysates were probed by protein immunoblot with polyclonal antisera to KatG, and KatG(-) lysates were screened by two-dimensional gel electrophoresis for the up-regulation of AhpC.
  • 24
    • 15844417430 scopus 로고    scopus 로고
    • note
    • We thank Y. Zhu, J. Morgenroth, and D. Crane for technical assistance; E. Tolentino and B. Mansfield and the PathoGenesis Microchemistry Facility for help with sequencing; and J. Reid, S. Altman, L. Shapiro, S. Benkovic, H. Caldwell, and R. Belland for careful review of the manuscript.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.