Purification and characterization of catalytic domains of gelatinase a with or without fibronectin insert for high-throughput inhibitor screening

Protein Expression and Purification

Volumn 27, Issue 1, 2003, Pages 63-74

  Cheng, Donghang ^a   Shen, Qiang ^a   Nan, Fajun ^a   Qian, Zhen ^a   Ye, Qi Zhuang ^a,b  

^a SHANGHAI INSTITUTE OF MATERIA MEDICA   (China)

^b UNIVERSITY OF KANSAS   (United States)

Author keywords

Catalytic domain; Fibronectin; Gene expression; High throughput screening; Human gelatinase A; Matrix metalloproteinase

Indexed keywords

BACTERIA (MICROORGANISMS);

CALCIUM; CHELATING AGENT; ENZYME INHIBITOR; FIBRONECTIN; GELATINASE A; MACROGOL DERIVATIVE; POLIDOCANOL; ZINC;

AMINO ACID SEQUENCE; ARTICLE; CHEMICAL STRUCTURE; CHEMISTRY; DRUG ANTAGONISM; DRUG EFFECT; ENZYME ACTIVE SITE; ESCHERICHIA COLI; GENETICS; HUMAN; IC 50; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; OSMOLARITY; PROTEIN RENATURATION;

AMINO ACID SEQUENCE; CALCIUM; CATALYTIC DOMAIN; CHELATING AGENTS; ENZYME INHIBITORS; ESCHERICHIA COLI; FIBRONECTINS; HUMANS; INHIBITORY CONCENTRATION 50; MATRIX METALLOPROTEINASE 2; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; OSMOLAR CONCENTRATION; POLYETHYLENE GLYCOLS; PROTEIN RENATURATION; ZINC;

EID: 0042284093     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1046-5928(02)00530-2     Document Type: Article

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