Structure of human pro-matrix metalloproteinase-2: Activation mechanism revealed

Science

Volumn 284, Issue 5420, 1999, Pages 1667-1670

  Morgunova, Ekaterina ^a   Tuuttila, Ari ^a   Bergmann, Ulrich ^a   Isupov, Mikhail ^b   Lindqvist, Ylva ^a   Schneider, Gunter ^a   Tryggvason, Karl ^a  

^a KAROLINSKA INSTITUTE   (Sweden)

^b UNIVERSITY OF EXETER   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

FIBRONECTIN; MATRIX METALLOPROTEINASE;

ARTICLE; CONFORMATIONAL TRANSITION; CONNECTIVE TISSUE DISEASE; ENZYME ACTIVATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME MECHANISM; ENZYME STABILITY; ENZYME STRUCTURE; EXTRACELLULAR MATRIX; HUMAN; HYDROPHOBICITY; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN TARGETING; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; CATALYTIC DOMAIN; ENZYME ACTIVATION; ENZYME PRECURSORS; FIBRONECTINS; GELATINASES; HEMOPEXIN; HUMANS; HYDROGEN BONDING; MATRIX METALLOPROTEINASE 2; METALLOENDOPEPTIDASES; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN STRUCTURE, SECONDARY;

EID: 0033523040     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.284.5420.1667     Document Type: Article

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42. XDS [W. Kabsch, J. Appl. Crystallogr. 21, 916 (1988)]; programs [CCP4, Collaborative Computational Project No. 4, Daresbury, UK, Acta Crystallogr. D 50, 760 (1994)]; SFCHECK [A. A. Vagin, J. Richelle, S. J. Wodak, ibid. 55, 191 (1999)]; AMORE [J. Navaza, Acta Crystallogr. A 50, 157 (1994)]; O [T. A. Jones, J.-Y. Zou, S. W. Cowan, M. Kjeldgaard, ibid. 47, 110 (1991)]; and X-PLOR [A. T. Brünger, X-PLOR, Version 3.1: A System for X-Ray Crystallography and NMR (Yale Univ. Press, New Haven, CT, 1992)].
43. XDS [W. Kabsch, J. Appl. Crystallogr. 21, 916 (1988)]; programs [CCP4, Collaborative Computational Project No. 4, Daresbury, UK, Acta Crystallogr. D 50, 760 (1994)]; SFCHECK [A. A. Vagin, J. Richelle, S. J. Wodak, ibid. 55, 191 (1999)]; AMORE [J. Navaza, Acta Crystallogr. A 50, 157 (1994)]; O [T. A. Jones, J.-Y. Zou, S. W. Cowan, M. Kjeldgaard, ibid. 47, 110 (1991)]; and X-PLOR [A. T. Brünger, X-PLOR, Version 3.1: A System for X-Ray Crystallography and NMR (Yale Univ. Press, New Haven, CT, 1992)].
44. XDS [W. Kabsch, J. Appl. Crystallogr. 21, 916 (1988)]; programs [CCP4, Collaborative Computational Project No. 4, Daresbury, UK, Acta Crystallogr. D 50, 760 (1994)]; SFCHECK [A. A. Vagin, J. Richelle, S. J. Wodak, ibid. 55, 191 (1999)]; AMORE [J. Navaza, Acta Crystallogr. A 50, 157 (1994)]; O [T. A. Jones, J.-Y. Zou, S. W. Cowan, M. Kjeldgaard, ibid. 47, 110 (1991)]; and X-PLOR [A. T. Brünger, X-PLOR, Version 3.1: A System for X-Ray Crystallography and NMR (Yale Univ. Press, New Haven, CT, 1992)].
45. XDS [W. Kabsch, J. Appl. Crystallogr. 21, 916 (1988)]; programs [CCP4, Collaborative Computational Project No. 4, Daresbury, UK, Acta Crystallogr. D 50, 760 (1994)]; SFCHECK [A. A. Vagin, J. Richelle, S. J. Wodak, ibid. 55, 191 (1999)]; AMORE [J. Navaza, Acta Crystallogr. A 50, 157 (1994)]; O [T. A. Jones, J.-Y. Zou, S. W. Cowan, M. Kjeldgaard, ibid. 47, 110 (1991)]; and X-PLOR [A. T. Brünger, X-PLOR, Version 3.1: A System for X-Ray Crystallography and NMR (Yale Univ. Press, New Haven, CT, 1992)].
46. XDS [W. Kabsch, J. Appl. Crystallogr. 21, 916 (1988)]; programs [CCP4, Collaborative Computational Project No. 4, Daresbury, UK, Acta Crystallogr. D 50, 760 (1994)]; SFCHECK [A. A. Vagin, J. Richelle, S. J. Wodak, ibid. 55, 191 (1999)]; AMORE [J. Navaza, Acta Crystallogr. A 50, 157 (1994)]; O [T. A. Jones, J.-Y. Zou, S. W. Cowan, M. Kjeldgaard, ibid. 47, 110 (1991)]; and X-PLOR [A. T. Brünger, X-PLOR, Version 3.1: A System for X-Ray Crystallography and NMR (Yale Univ. Press, New Haven, CT, 1992)].
47. Figures 1, 2A, 3, A and B, and 4A were made with MOLSCRIPT [P. J. Kraulis, J. Appl. Crystallogr. 24, 946 (1991)] and RASTER3D [E. A. Merrit and M. E. P. Murphy, Acta Crystallogr. D 50, 869 (1994)] . Figures 3, C and D, and 4B were made with GRASP [A. Nichols, K. A. Sharp, B. Honig, Proteins 11, 281 (1991)].
48. Figures 1, 2A, 3, A and B, and 4A were made with MOLSCRIPT [P. J. Kraulis, J. Appl. Crystallogr. 24, 946 (1991)] and RASTER3D [E. A. Merrit and M. E. P. Murphy, Acta Crystallogr. D 50, 869 (1994)] . Figures 3, C and D, and 4B were made with GRASP [A. Nichols, K. A. Sharp, B. Honig, Proteins 11, 281 (1991)].
49. Figures 1, 2A, 3, A and B, and 4A were made with MOLSCRIPT [P. J. Kraulis, J. Appl. Crystallogr. 24, 946 (1991)] and RASTER3D [E. A. Merrit and M. E. P. Murphy, Acta Crystallogr. D 50, 869 (1994)] . Figures 3, C and D, and 4B were made with GRASP [A. Nichols, K. A. Sharp, B. Honig, Proteins 11, 281 (1991)].
50. Supported by grants from the Swedish Cancer Foundation, EC project BMH4-CT 96-0012, Novo Nordisk Foundation, and Hedlund's Foundation. We thank Tiina Berg, Ilkka Miinalainen, and Kristian Tryggvason for technical assistance with insect cell cultures. We are also grateful to Richard Kahn and Tatjana Sandalova for assistance with the data collection. Beam time was provided by the ESRF.