The aminoglycoside 6′-N-acetyltransferase type Ib encoded by Tn1331 is evenly distributed within the cell's cytoplasm

Antimicrobial Agents and Chemotherapy

Volumn 47, Issue 9, 2003, Pages 2897-2902

  Dery, Ken J ^a,c   Søballe, Britta ^b   Witherspoon, Mavee S L ^a   Bui, Duyen ^a,b   Koch, Robert ^a   Sherratt, David J ^b   Tolmasky, Marcelo E ^a,b  

^a CALIFORNIA STATE UNIVERSITY   (United States)

^b UNIVERSITY OF OXFORD   (United Kingdom)

^c CITY OF HOPE NATIONAL MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACYLTRANSFERASE; AMINOGLYCOSIDE 6' N ACETYLTRANSFERASE IB; AMINOGLYCOSIDE ANTIBIOTIC AGENT; BETA LACTAM ANTIBIOTIC; BETA LACTAMASE; SIGNAL PEPTIDE; UNCLASSIFIED DRUG; ALKALINE PHOSPHATASE; AMINOGLYCOSIDE N(6') ACETYLTRANSFERASE; AMINOGLYCOSIDE N(6')-ACETYLTRANSFERASE; BACTERIAL DNA; BACTERIAL PROTEIN;

AAC(6') IB GENE; AMINO ACID SEQUENCE; ARTICLE; BACTERIAL GENE; BLA OXA9 GENE; BLA TEM1 GENE; CELL FRACTIONATION; CODON; CYTOPLASM; ENZYME LOCALIZATION; ESCHERICHIA COLI; FLUORESCENCE MICROSCOPY; GENE DUPLICATION; GENE FUSION; GENE ISOLATION; GENE MAPPING; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PHOA GENE; PRIORITY JOURNAL; PROMOTER REGION; SERRATIA MARCESCENS; SPHEROPLAST; TRANSLATION INITIATION; TRANSLATION REGULATION; TRANSPOSON; TRANSPOSON TN1331; ENZYMOLOGY; GENETICS; IMMUNOBLOTTING; METABOLISM; MOLECULAR GENETICS; PLASMID;

ACETYLTRANSFERASES; ALKALINE PHOSPHATASE; BACTERIAL PROTEINS; CYTOPLASM; DNA, BACTERIAL; ESCHERICHIA COLI; IMMUNOBLOTTING; MICROSCOPY, FLUORESCENCE; MOLECULAR SEQUENCE DATA; PLASMIDS; SUBCELLULAR FRACTIONS;

EID: 0041922659     PISSN: 00664804     EISSN: None     Source Type: Journal    
DOI: 10.1128/AAC.47.9.2897-2902.2003     Document Type: Article

References (51)

1. Adler, J., and E. Bibi. 2002. Membrane topology of the multidrug transporter MdfA: complementary gene fusion studies reveal a nonessential C-terminal domain. J. Bacteriol. 184:3313-3320.
2. Bachmann, B. 1996. Derivations and genotypes of some mutant derivatives of Escherichia coli K-12, p. 2460-2495. In F. C. Neidhardt, R. Curtiss III, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasanik, W. S. Reznikoff, M. Riley, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., vol. 2. ASM Press, Washington, D.C.
3. Barre, F. X., M. Aroyo, S. D. Colloms, A. Helfrich, F. Cornet, and D. J. Sherratt. 2000. FtsK functions in the processing of a Holliday junction intermediate during bacterial chromosome segregation. Genes Dev. 14:2976-2988.
4. Bowden, G. A., and G. Georgiou. 1990. Folding and aggregation of beta-lactamase in the periplasmic space of Escherichia coli. J. Biol. Chem. 265:16760-16766.
5. Boyer, H. W., and D. Roulland-Dussoix. 1969. A complementation analysis of the restriction and modification of DNA in Escherichia coli. J. Mol. Biol. 41:459-472.
6. Casin, I., F. Bordon, P. Bertin, A. Coutrot, I. Podglajen, R. Brasseur, and E. Collatz. 1998. Aminoglycoside 6′-N-acetylransferase variants of the Ib type with altered substrate profile in clinical isolates of Enterobacter cloacae and Citrobacter freundii. Antimicrob. Agents Chemother. 42:209-215.
7. PER-1-carrying plasmid pSTI1 encodes an extended-spectrum aminoglycoside 6′-N-acetyltransferase of type Ib. Antimicrob. Agents Chemother. 47:697-703.
8. Chavideh, R., S. Sholly, D. Panaite, and M. E. Tolmasky. 1999. Effects of F171 mutations in the 6′-N-acetyltransferase type Ib [AAC(6′)-Ib] enzyme on susceptibility to aminoglycosides. Antimicrob. Agents Chemother. 43:2811-2812.
9. Cohen, S., A. Chang, and L. Hsu. 1972. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proc. Natl. Acad. Sci. USA 69:2110-2114.
10. Dalbey, R. E., and C. Robinson. 1999. Protein translocation into and across the bacterial plasma membrane and the plant thylakoid membrane. Trends Biochem. Sci. 24:17-22.
11. Danese, P. N., and T. J. Silhavy. 1998. Targeting and assembly of periplasmic and outer-membrane proteins in Escherichia coli. Annu. Rev. Genet. 32:59-94.
12. DeLisa, M. P., P. Samuelson, T. Palmer, and G. Georgiou. 2002. Genetic analysis of the twin-arginine translocator secretion pathway in bacteria. J. Biol. Chem. 277:29825-29831.
13. Ekman, D. R., E. L. DiGiammarino, E. Wright, E. D. Witter, and E. H. Serpersu. 2001. Cloning, overexpression, and purification of aminoglycoside antibiotic nucleotidyltransferase (2″)-Ia: conformational studies with bound substrates. Biochemistry 40:7017-7024.
14. Feucht, A., and P. J. Lewis. 2001. Improved plasmid vectors for the production of multiple fluorescent protein fusions in Bacillus subtilis. Gene 264:289-297.
15. Fong, D. H., and A. M. Berghuis. 2002. Substrate promiscuity of an aminoglycoside antibiotic resistance enzyme via target mimicry. EMBO J. 21:2323-2331.
16. BAD promoter. J. Bacteriol. 177:4121-4130.
17. Kadonaga, J. T., A. Pluckthun, and J. R. Knowles. 1985. Signal sequence mutants of beta-lactamase. J. Biol. Chem. 260:16192-16199.
18. Kotra, L. P., J. Haddad, and S. Mobashery. 2000. Aminoglycosides: perspectives on mechanisms of action and resistance and strategies to counter resistance. Antimicrob. Agents Chemother. 44:3249-3256.
19. Manoil, C., and J. Beckwith. 1985. TnphoA: a transposon probe for protein export signals. Proc. Natl. Acad. Sci. USA 82:8129-8133.
20. Manting, E. H., and A. J. Driessen. 2000. Escherichia coli translocase: the unravelling of a molecular machine. Mol. Microbiol. 37:226-238.
21. Neuwald, A. F., and D. Landsman. 1997. GCN5-related histone N-acetyltransferases belong to a diverse superfamily that includes the yeast SPT10 protein. Trends Biochem. Sci. 22:154-155.
22. Nobuta, K., M. E. Tolmasky, L. M. Crosa, and J. H. Crosa. 1988. Sequencing and expression of the 6′-N-acetyltransferase gene of transposon Tn1331 from Klebsiella pneumoniae. J. Bacteriol. 170:3769-3773.
23. O'Farrell, P. H. 1975. High resolution two-dimensional electrophoresis of proteins. J. Biol. Chem. 250:4007-4021.
24. Panaite, D. M., and M. E. Tolmasky. 1998. Characterization of mutants of the 6′-N-acetyltransferase encoded by the multiresistance transposon Tn1331: effect of Phen171-to-Leu171 and Tyr80-to-Cys80 substitutions. Plasmid 39:123-133.
25. Perlin, M. H., and S. A. Lerner. 1981. Localization of an amikacin 3′-phosphotransferase in Escherichia coli. J. Bacteriol. 147:320-325.
26. Pluckthun, A., and J. R. Knowles. 1987. The consequences of stepwise deletions from the signal-processing site of beta-lactamase. J. Biol. Chem. 262:3951-3957.
27. Pourcher, T., E. Bibi, H. R. Kaback, and G. Leblanc. 1996. Membrane topology of the melibiose permease of Escherichia coli studied by melB-phoA fusion analysis. Biochemistry 35:4161-4168.
28. Rather, P. N., H. Munayyer, P. A. Mann, R. S. Hare, G. H. Miller, and K. J. Shaw. 1992. Genetic analysis of bacterial acetyltransferases: identification of amino acids determining the specificities of the aminoglycoside 6′-N-acetyltransferase Ib and IIa proteins. J. Bacteriol. 174:3196-3203.
29. Samuelson, J. C., M. Chen, F. Jiang, I. Moller, M. Wiedmann, A. Kuhn, G. J. Phillips, and R. E. Dalbey. 2000. YidC mediates membrane protein insertion in bacteria. Nature 406:637-641.
30. Sarno, R., G. McGillivary, D. J. Sherratt, L. Actis, and M. E. Tolmasky. 2002. Complete sequence of Klebsiella pneumoniae multiresistance plasmid pJHCMW1. Antimicrob. Agents Chemother. 46:3422-3427.
31. Shaw, K. J., P. N. Rather, R. S. Hare, and G. H. Miller. 1993. Molecular genetics of aminoglycoside resistance genes and familial relationships of the aminoglycoside-modifying enzymes. Microbiol. Rev. 57:138-163.
32. Shmara, A., N. Weinsetel, K. J. Dery, R. Chavideh, and M. E. Tolmasky. 2001. Systematic analysis of a conserved region of the aminoglycoside 6′-N-acetyltransferase type Ib. Antimicrob. Agents Chemother. 45:3287-3292.
33. Strom, M. S., and S. Lory. 1987. Mapping of export signals of Pseudomonas aeruginosa pilin with alkaline phosphatase fusions. J. Bacteriol. 169:3181-3188.
34. Studier, F., A. Rosenberg, J. Dunn, and J. Dubendorff. 1990. Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol. 185:60-89.
35. Summers, D. K., and D. J. Sherratt. 1988. Resolution of ColE1 dimers requires a DNA sequence implicated in the three-dimensional organization of the cer site. EMBO J. 7:851-858.
36. Tabor, S., and C. C. Richardson. 1985. A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes. Proc. Natl. Acad. Sci. USA 82:1074-1078.
37. Thomas, J. D., R. A. Daniel, J. Errington, and C. Robinson. 2001. Export of active green fluorescent protein to the periplasm by the twin-arginine translocase (Tat) pathway in Escherichia coli. Mol. Microbiol. 39:47-53.
38. Thompson, P. R., J. Schwartzenhauer, D. W. Hughes, A. M. Berghuis, and G. D. Wright. 1999. The COOH terminus of aminoglycoside phosphotransferase (3′)-IIIa is critical for antibiotic recognition and resistance. J. Biol. Chem. 274:30697-30706.
39. Tolmasky, M. E. 1990. Sequencing and expression of aadA, bla, and tnpR from the multiresistance transposon Tn1331. Plasmid 24:218-226.
40. Tolmasky, M. E., R. M. Chamorro, J. H. Crosa, and P. M. Marini. 1988. Transposon-mediated amikacin resistance in Klebsiella pneumoniae. Antimicrob. Agents Chemother. 32:1416-1420.
41. Tolmasky, M. E., and J. H. Crosa. 1993. Genetic organization of antibiotic resistance genes (aac(6′)-Ib, aadA, and oxa9) in the multiresistance transposon Tn1331. Plasmid 29:31-40.
42. Tolmasky, M. E., and J. H. Crosa. 1987. Tn1331, a novel multiresistance transposon encoding resistance to amikacin and ampicillin in Klebsiella pneumoniae. Antimicrob. Agents Chemother, 31:1955-1960.
43. Tran van Nhieu, G., and E. Collatz. 1987. Primary structure of an aminoglycoside 6′-N-acetyltransferase AAC(6′)-4, fused in vivo with the signal peptide of the Tn3-encoded beta-lactamase. J. Bacteriol. 169:5708-5714.
44. Vazquez-Laslop, N., H. Lee, R. Hu, and A. A. Neyfakh. 2001. Molecular sieve mechanism of selective release of cytoplasmic proteins by osmotically shocked Escherichia coli. J. Bacteriol. 183:2399-2404.
45. Vetting, M. W., S. S. Hegde, F. Javid-Majd, J. S. Blanchard, and S. L. Roderick. 2002. Aminoglycoside 2′-N-acetyltransferase from Mycobacterium tuberculosis in complex with coenzyme A and aminoglycoside substrates. Nat. Struct. Biol. 9:653-658.
46. Vliegenthart, J. S., P. A. Ketelaar-van Gaalen, J. Eelhart, and J. A. van de Klundert. 1991. Localisation of the aminoglycoside-(3)-N-acetyltransferase isoenzyme II in Escherichia coli. FEMS Microbiol. Lett. 65:101-105.
47. Witholt, B., M. Boekhout, M. Brock, J. Kingma, H. V. Heerikhuizen, and L. D. Leij. 1976. An efficient and reproducible procedure for the formation of spheroplasts from variously grown Escherichia coli. Anal. Biochem. 74:160-170.
48. Wolf, E., A. Vassilev, Y. Makino, A. Sali, Y. Nakatani, and S. K. Burley. 1998. Crystal structure of a GCN5-related N-acetyltransferase: Serratia marcescens aminoglycoside 3-N-acetyltransferase. Cell 94:439-449.
49. Woods, G., and J. Washington. 1995. Antibacterial susceptibility tests: dilution and disk diffusion methods, p. 1327-1341. In P. R. Murray, E. J. Baron, M. A. Pfaller, F. C. Tenover, and R. H. Yolken (ed.), Manual of clinical microbiology, 6th ed. ASM Press, Washington, D.C.
50. Wright, G. D. 1999. Aminoglycoside-modifying enzymes. Curr. Opin. Microbiol. 2:499-503.
51. Wybenga-Groot, L. E., K. Draker, G. D. Wright, and A. M. Berghuis. 1999. Crystal structure of an aminoglycoside 6′-N-acetyltransferase: defining the GCN5-related N-acetyltransferase superfamily fold. Struct. Fold Des. 7:497-507.