Mutation of residues in the coenzyme binding pocket of Dopa decarboxylase: Effects on catalytic properties

European Journal of Biochemistry

Volumn 268, Issue 10, 2001, Pages 2975-2981

  Bertoldi, Mariarita ^a   Castellani, Silvia ^a   Voltattorni, Carla Borri ^a  

^a UNIVERSITY OF VERONA   (Italy)

Author keywords

Dopa decarboxylase; Molecular oxygen; Mutants; Nonoxidative and oxidative decarboxylation; Pyridoxal 5 phosphate

Indexed keywords

AROMATIC LEVO AMINO ACID DECARBOXYLASE; PYRIDOXAL 5 PHOSPHATE;

AEROBIC METABOLISM; ARTICLE; BINDING SITE; CATALYSIS; DECARBOXYLATION; DISSOCIATION CONSTANT; ENZYME ACTIVITY; ENZYME BINDING; MUTATION; NONHUMAN; OXIDATION; OXYGEN AFFINITY; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN LOCALIZATION;

ALGORITHMS; ANIMALS; BINDING SITES; CATALYSIS; CIRCULAR DICHROISM; DOPA DECARBOXYLASE; DOSE-RESPONSE RELATIONSHIP, DRUG; KINETICS; LEVODOPA; LIVER; MODELS, CHEMICAL; MUTAGENESIS, SITE-DIRECTED; MUTATION; OXYGEN; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY; RATS; SEROTONIN; TIME FACTORS;

EID: 0034826435     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.2001.02187.x     Document Type: Article

References (20)

1. Decarboxylation-dependent transamination catalyzed by mammalian 3,4-dihydroxyphenylalanine decarboxylase
2. Reaction of dopa decarboxylase with L-aromatic amino acids under aerobic and anaerobic conditions
3. Mechanism-based inactivation of Dopa decarboxylase by serotonin
4. Reaction of Dopa decarboxylase with α-methyldopa leads to an oxidative deamination producing 3,4-dihydrophenylacetone, an active site directed affinity label
5. Crystallographic structure of a PLP-dependent ornithine decarboxylase from Lactobacillus 30a to 3.0 Å resolution
6. Structural motifs for pyridoxal 5′-phosphate binding in decarboxylases: An analysis based on the crystal structure of the Lactobacillus 30a ornithine decarboxylase
7. Structure modelling and site-directed mutagenesis of the rat aromatic L-amino acid pyridoxal 5′-phosphate-dependent decarboxylase: A functional study
8. Site-directed mutagenesis by overlap extension using the polymerase chain reaction
9. Cloning and expression of pig kidney dopa decarboxylase: Comparison of the naturally and recombinant enzymes
10. Dissociation, unfolding and refolding trials of pig kidney 3,4-dihydroxyphenylalanine decarboxylase
11. A spectro-photometric assay for Drosophila Dopa decarboxylase
12. An investigation of the assay of dopamine trinitrobenzenesulphonic acid
13. Reaction specificity of native and nicked 3,4-dihydroxyphenylalanine decarboxylase
14. Mutation of cysteine 111 in Dopa decarboxylase leads to active site perturbation
15. Quantitative analysis of protein far UV circular dichroism spectra by neural networks
16. Functionally important residues of aromatic L-amino acid decarboxylase probed by sequence alignment and site-directed mutagenesis
17. Substitution of apolar residues in the active site of aspartate aminotransferase by histidine. Effects on reaction and substrate specificity
18. Conversion of aspartate aminotransferase into an L-aspartate β-decarboxylase by a triple active-site mutation
19. Altering the reaction specificity of eukaryotic ornithine decarboxylase