RNA-dependent RNA polymerase activity of classical swine fever virus NS5B protein expressed in natural host cells

Acta Virologica

Volumn 47, Issue 2, 2003, Pages 79-85

  Xiao, M ^a,b   Chen, J ^b   Li, B ^b  

^a SHANGHAI NORMAL UNIVERSITY   (China)

^b FUDAN UNIVERSITY   (China)

Author keywords

Classical swine fever virus; NS5B gene; RNA dependent RNA polymerase

Indexed keywords

CYSTINE; DETERGENT; GLYCEROL; NS5B PROTEIN; PROTEIN; RNA DIRECTED RNA POLYMERASE; SODIUM CHLORIDE; THYMINE; UNCLASSIFIED DRUG;

ARTICLE; ENZYME ACTIVITY; HOST CELL; KIDNEY CELL; NONHUMAN; NS5B GENE; PESTIVIRUS; PROTEIN EXPRESSION; RNA SYNTHESIS; SITE DIRECTED MUTAGENESIS; SWINE; VIRUS GENE; VIRUS GENOME; WESTERN BLOTTING;

ANIMALS; BLOTTING, WESTERN; CELL LINE; CLASSICAL SWINE FEVER VIRUS; GENE EXPRESSION; GENOME, VIRAL; RNA REPLICASE; RNA, VIRAL; SWINE; TEMPLATES, GENETIC; VIRAL NONSTRUCTURAL PROTEINS;

ANIMALIA; CLASSICAL SWINE FEVER VIRUS; DNA VIRUSES; PESTIVIRUS; SUIDAE; SUS SCROFA;

EID: 0041360395     PISSN: 0001723X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (30)

1. Behrens SE, Tornei L, Francesco RD (1996): Identification and Properties of the RNA-dependent RNA polemerase of hepatitis C virus. EMBO J. 15, 12-22.
2. Deng R, Brock KV (1993): 5′ and 3′ untranslated regions of pestivirus genome: primary and secondary structure analyses. Nucleic Acids Res. 21, 1949-1957.
3. Ferrari E, Jacquelyz WM, Fang JM, Barrudy, BYZ, Lau J, Hong Z (1999): Characterization of soluble hepatitis C virus RNA-dependent RNA polymerase expressed in Escherichia coli. J. Virol. 73, 1649-1654.
4. Francki RI, Fauquet DL, Knudson DL, Brown F (1991): Classification and nomenclature of viruses. Arch. Virol. 136 (Suppl. 2), 223-233.
5. Hulst MM, van Gennip HGP, Moormann RJM (2000): Passage of classical swine fever virus in cultured swine kidney cells selects virus variants that bind to hearan sulfate due to a single amino acid change in envelope protein erns. J. Virol. 74, 9553-9561.
6. Jin L, Peterson DL (1995): Expression, isolation, and characterization of the hepatitis C virus ATPase/RNA helicase. Arch. Biochem. Biophys. 323, 47-53.
7. Kao CC, Del Vecchio AM, Zhong W (1999): De novo initiation of RNA synthesis by a recombinant flaviridae RNA-depentent RNA polymerase. Virology, 253, 1-7.
8. Le SY, Sonenberg N, Maizel JJV (1995): Unusual folding regions and ribosome landing pad within hepatitis C virus and pestivirus RNAs. Gene 154, 137-143.
9. Lohmann V, Körner F, Herian U, Bartenschlager R (1997): Biochemical Properties of hepatitis C virus NS5B RNA-dependent RNA polymerase and Identification of amino acid sequence motifs essential for enzymatic activity. J. Virol. 71, 8416-8428.
10. Lohmann V, Overton H, Bartenschlager R (1999): Selective stimulation of hepatitis C virus and pestivirus NS5B RNA polymerase activity by GTP. J. Biol. Chem. 274, 10807-10815.
11. Meyers G, Rümenapf T, Thiel H J (1989): Molecular cloning and nucleotide sequence of the genome of classical swine fever virus. Virology 171, 555-567.
12. Meyers G, Thiel HJ, Rümenapf T (1996): Classical swine fever virus: recovery of infectious viruses from cDNA constructs and generation of recombinant cytopathogenic defective interfering particles. J. Virol. 70, 1588-1596.
13. Mondelli MU, Cerino A, Boender P, Oudshoorn P, Middeldorp J, Fipaldini C, Monica N, Habets W (1994): Significance of the immune response to a major, conformational B-cell epitope on the hepatitis C virus NS3 region defined by a human monoclonal antibody. J. Virol. 68, 4829-4836.
14. Moormann RJM, van Gennip HGP, Miedema GKW, Hulst MM, van Rijn PA (1996): Infectious RNA transcribed from an engineered full-length cDNA template of the genome of a pestivirus. J. Virol. 70, 763-770.
15. Moser C, Stettler P, Trutschin JD, Hofmann MA (1999): Cytopathogenic and noncytopathogenic RNA replicons of classical swine fever virus. J. Virol. 73, 7784-7794.
16. O'Reilly EK, Kao CC (1998): Analysis of RNA-dependent RNA polymerase structure and function as guided by known polymerase structures and computer prediction of secondary structure. Virology 252, 287-303.
17. Ranjith-Kumar CT, Gutshall L, Kim MJ, Sarisky RT, Kao CC (2002): Requirements for de novo initiation of RNA synthesis by recombinant flaviviral RNA-dependent RNA polymerases. J. Virol. 76, 12526-12536,
18. Ronald H Al, Yiping Xie, Yuhuan Wang, Cyrt H, Hagedorn CH (1998): Expression of recombinant hepatitis C virus nonstructural protein 5B in Escherichia coli. Virus Res, 53, 141-149,
19. Ruggli N, Tratschin JD, Mittelholrer C, Hofmann MA (1996): Nucleotide sequence of classical swine fever virus strain Alfart/187 and transcription of infectious RNA from stably cloned full-length cDNA, J. Virol, 70, 3478-3487.
20. Sambrook J, Fritsch EF, Maniatis T (1989): Molecular Cloning: A Laboratory Manual. 2nd ed, Cold Spring Harbor Laboratory, Cold Spring Harbor.
21. Steffen S, Thiel HJ, Behrens SE (1999): The RNA-dependent RNA polymerase of different members of the family Flaviviridae exhibit similar properties in vitro. J. Gen. Virol. 80, 2583-2590.
22. Tratschin JD, Moser C, Ruggli N, Hofmann MA (1998): Classical swine fever virus leader proteinase Npro is not required for viral replication in cell culture. J. Virol. 72, 7681-7684.
23. van Regenmortel MHV, Fauquet CM, Bishop DHL (2000): Virus Taxonomy. Seventh Report of the International Committee on Tatonomy of Viruses. Academic Press, San Diego-San Frascisco-New York-Boston-London-Sydney-Tokyo.
24. Warrener P, Collett MS (1995): Pestivirus NS3 (p80) protein possesses RNA helicase activity. J. Virol, 69, 1720-1726.
25. Wengler G, Wengler G (1991): The carboxy-terminal part of the NS3 protein of the West Nile flavivirus can be isolated as a soluble protein after proteolytic cleavage and represents an RNA-stimulated NTPase. Virology 184, 707-715.
26. Yu H, Grassmann CW, Behrens SE (1999): Sequence and structural elements at the 3′ terminus of bovine viral diarrhea virus genomic RNA: functional role during RNA replication. J. Virol. 73, 3638-3648.
27. Xiao M, Zhi ZZ, Zhang CY (2001): Qualitative, quantitative and structural analysis of non-coding regions of Classical Swine Fever Virus genome, Chinese Sci. Bull, 46, 1251-1257.
28. Xiao M, Zhi ZZ, Jueping L, Zhang CY (2002a): Prediction of recognition sites of Classical Swine Fever Virus genomic replication with information analysis. Mol, Biol, 36, 34-43.
29. Xiao M, Zhang CY, Pan ZS, Wu HX, Guo JQ (2002b): Classical Swine Fever Virus NS5B-GFP Fusion Possesses an RNA-dependent RNA Polymerase Activity. Arch. Virol. 147, 1779-1787.
30. Zhong W, Gutshall LL, Del Vecchio AM (1998): Identification and characterization of an RNA-dependent RNA polymerase activity within the nonstructural protein 5B region of bovine viral diarrhea virus. J. Virol. 72, 9365-936.