Catalytic activity of the D38A mutant of 3-oxo-Δ5-steroid isomerase: Recruitment of aspartate-99 as the base

Biochemistry

Volumn 39, Issue 12, 2000, Pages 3351-3359

  Hénot, Frédéric ^a   Pollack, Ralph M ^b  

^a UNIVERSITY OF MARYLAND   (United States)

^b UNIVERSITY OF MARYLAND BIOTECHNOLOGY INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ASPARTIC ACID; STEROID DELTA ISOMERASE;

ARTICLE; CATALYSIS; COMAMONAS TESTOSTERONI; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME KINETICS; ISOMERISM; PRIORITY JOURNAL;

ALANINE; ASPARTIC ACID; BINDING SITES; CATALYSIS; COMAMONAS TESTOSTERONI; ELECTROPHORESIS, POLYACRYLAMIDE GEL; EQUILENIN; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; KINETICS; MUTAGENESIS, SITE-DIRECTED; PLASMIDS; SPECTROPHOTOMETRY, ULTRAVIOLET; STEROID ISOMERASES; TITRIMETRY;

BACTERIA (MICROORGANISMS); COMAMONAS TESTOSTERONI; FELIS CATUS;

EID: 0040182818     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi9922446     Document Type: Article

References (53)

1. Hawkinson, D. C., Eames, T. C. M., and Pollack, R. M. (1991) Biochemistry 30, 10849-10858.
2. Kuliopulos, A., Mildvan, A. S., Shortle, D., and Talalay, P. (1989) Biochemists 28, 149-159.
3. Benisek, W. F., Ogez, J. R., and Smith, S. B. (1980) Ann. N.Y. Acad. Sci. 346, 115-130.
4. Zawrotny, M. E., Hawkinson, D. C., Blotny, G., and Pollack, R. M. (1996) Biochemistry 35, 6438-6442.
5. Zeng, B., and Pollack, R. M. (1991) J. Am. Chem. Soc. 113, 3838-3842.
6. Zeng, B., Bounds, P. L., Steiner, R. F., and Pollack, R. M. (1992) Biochemistry 31, 1521-1528.
7. Petrounia, I., and Pollack, R. M. (1998) Biochemistry 37, 700-705.
8. Zhao, Q., Li, Y.-K., Mildvan, A. S., and Talalay, P. (1995) Biochemistry 34, 426-434.
9. Zhao, Q., Abeygunawardana, C., Talalay, P., and Mildvan, A. S. (1996) Proc. Natl. Acad. Sci. U.S.A. 93, 8220-8224.
10. Wu, Z. R., Ebrahimian, S., Zawrotny, M. E., Thornburg, L. D., Perez-Alvarado, G. C., Brothers, P., Pollack, R. M., and Summers, M. F. (1997) Science 276, 415-418.
11. Thornburg, L. D., Hénot, F., Bash, D. P., Hawkinson, D. C., Bartel, S. D., and Pollack, R. M. (1998) Biochemistry 37, 10499-10506.
12. Zhao, Q., Abeygunawardana, C., Gittis, A. G., and Mildvan, A. S., (1997) Biochemistry 36, 14616-14626.
13. Cho, H.-S., Ha, N.-C., Choi, G., Kim, H.-J., Lee, D., Oh, K.-S., Kim, K. S., Lee, W., Choi, K. Y., and Oh, B.-H. (1999) J. Biol. Chem. 274, 32863-32868.
14. Pollack, R. M., Thornburg, L. D., Wu, Z. R., and Summers, M. F. (1999) Arch. Biochem. Biophys. 370, 9-15.
15. Xue, L. A., Kuliopulos, A., Mildvan, A. S., and Talalay, P. (1991) Biochemistry 30, 4991-4997.
16. Pollack, R. M., Zeng, B., Mack, J. P. G., and Eldin, S. (1989) J. Am. Chem. Soc. 111, 6419-6423.
17. Brooks, B., and Benisek, W. F. (1992) Biochem. Biophys. Res. Commun. 184, 1386-1392.
18. Laemmli, U. K. (1970) Nature 227, 680-685.
19. Kawahara, F. S., Wang, S.-F., and Talalay, P. (1962) J. Biol. Chem. 237, 1500-1506.
20. Schowen, K. B., and Schowen, R. L. (1982) Methods Enzymol. 87c, 551-606.
21. Kuliopulos, A., Talalay, P., and Mildvan, A. S. (1990) Biochemistry 29, 10271-10280.
22. Hawkinson, D. C., Pollack, R. M., and Ambulos, N. P., Jr. (1994) Biochemistry 33, 12172-12183.
23. Holman, C. H., and Benisek, W. F. (1995) Biochemistry 34, 14245-14253.
24. Xue, L., Talalay, P., and Mildvan, A. S. (1990) Biochemistry 29, 7491-7500.
25. Fersht, A. R. (1984) Enzyme Structure and Mechanism, 2nd ed., W. H. Freeman, New York.
26. Knowles, J. R. (1976) Crit. Rev. Biochem. 4, 165-173.
27. Cleland, W. W. (1977) Adv. Enzymol. Relat. Areas Mol. Biol. 45, 273-388.
28. Nilsson, A., and Lindskog, S. (1967) Eur. J. Biochem. 2, 309-317.
29. Pollack, R. M., Kayser, R. H., and Bevins, C. L. (1979) Biochem. Biophvs. Res. Commun. 91, 783-790.
30. Bevins, C. L., Bantia, S., Pollack, R. M., Bounds, P. L., and Kayser, R. H. (1984) J. Am. Chem. Soc. 106, 4957-4962.
31. Bounds, P. L., and Pollack, R. M. (1987) Biochemistry 26, 2263-2269.
32. Weintraub, H., Alfsen, A., and Baulieu, E.-E. (1970) Eur. J. Biochem. 12, 217-221.
33. Pollack, R. M., Bantia, S., Bounds, P. L., and Koffman, B. D. (1986) Biochemistry 25, 1905-1911.
34. Steyaert, J., Hallenga, K., Wyns, L., and Stanssens, P. (1990) Biochemistry 29, 9064-9072.
35. Kim, Y., Misna, D., Monzingo, A. F., Ready, M. P., Frankel, A., and Robertus, J. D. (1992) Biochemistry 31, 3294-3296.
36. Mizuguchi, H., Cook, P. F., Tai, C.-H., Hasemann, C. A., and Uyeda, K. (1999) J. Biol. Chem. 274, 2166-2175.
37. Cho, H.-S., Choi, G., Choi, K. Y., and Oh, B.-H. (1998) Biochemistry 37, 8325-8330.
38. Sternberg, M. J. E., Hayes, F. R. F., Russel, A. J., Thomas, P. G., and Fersht, A. R. (1987) Nature 330, 86-88.
39. Jackson, S. E., and Fersht, A. R. (1993) Biochemistry 32, 13909-13916.
40. Loewenthal, R., Sancho, J., Reinikainen, T., and Fersht, A. R. (1993) J. Mol. Biol. 232, 574-583.
41. Nakamura, H. (1996) Q. Rev. Biophys. 29, 1-90.
42. McIntosh, L. P., Hand, G., Johnson, P. E., Joshi, M. D., Korner, M., Plesniak, L. A., Ziser, L., Wakarchuk, W. W., and Withers, S. G. (1996) Biochemistry 35, 9958-9966.
43. Inoue, M., Yamada, H., Yasukochi, T., Kuroki, R., Miki, T., Horiuchi, T., and Imoto, T. (1992) Biochemistry 31, 5545-5553.
44. Bartik, K., Redfield, C., and Dobson, C. M. (1994) Biophys. J. 66, 1180-1184.
45. Lederer, F. (1992) Protein Sci. 1, 540-548.
46. Gondry, M., and Lederer, F. (1996) Biochemistry 35, 8587-8594.
47. Li, Y. K., Kuliopulos, A., Mildvan, A. S., and Talalay, P. (1993) Biochemistry 32, 1816-1824.
48. Wang, S. F., Kawahara, F. S., and Talalay, P. (1963) J. Biol. Chem. 238, 576-585.
49. Eames, T. C., Pollack, R. M., and Steiner, R. F. (1989) Biochemistry 28, 6269-6275.
50. Wu, P., Li, Y.-K, Talalay, P., and Brand, L. (1994) Biochemistry 33, 1415-1422.
51. Bevins, C. L., Pollack, R. M., Kayser, R. L.; and Bounds, P. L. (1986) Biochemistry 25, 5159-5164.
52. Zawrotny, M. E. and Pollack, R. M., (1994) Biochemistry 33, 13896-13902.
53. Weintraub, H., Alfsen, A., Baulieu, E.-E. (1970) Eur. J. Biochem. 12, 217-221.