Analysis of the catalytic and binding residues of the diadenosine tetraphosphate pyrophosphohydrolase from Caenorhabditis elegans by site-directed mutagenesis

Journal of Biological Chemistry

Volumn 278, Issue 7, 2003, Pages 4435-4439

  Abdelghany, Hend M ^a   Bailey, Scott ^b   Blackburn, G Michael ^c   Rafferty, John B ^b   McLennan, Alexander G ^a,d  

^a UNIVERSITY OF LIVERPOOL   (United Kingdom)

^b Dept of Molec Biol/Biotechnology ^*  (United Kingdom)

^c UNIVERSITY OF SHEFFIELD   (United Kingdom)

^d Bass Center   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING ENERGY; CATALYSIS; MUTAGENESIS; PROTEINS;

COMPLEXES;

BIOCHEMISTRY;

ADENOSINE; ALANINE; DIADENOSINE TETRAPHOSPHATE; DNA (APURINIC OR APYRIMIDINIC SITE) LYASE; GLUTAMIC ACID; GLUTATHIONE TRANSFERASE; GLYCINE; HISTIDINE; HYBRID PROTEIN; HYDROLASE; INORGANIC PYROPHOSPHATASE; LYSINE; METHIONINE; MUTANT PROTEIN; NUDIX HYDROLASE; PHOSPHATE; UNCLASSIFIED DRUG; VALINE; ACID ANHYDRIDE HYDROLASE; BIS(5' NUCLEOSYL)TETRAPHOSPHATASE (ASYMMETRICAL); BIS(5'-NUCLEOSYL)TETRAPHOSPHATASE (ASYMMETRICAL);

AMINO ACID SUBSTITUTION; ARTICLE; BINDING SITE; CAENORHABDITIS ELEGANS; CATALYSIS; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENZYME ANALYSIS; ENZYME SPECIFICITY; ENZYME SUBSTRATE COMPLEX; MICHAELIS CONSTANT; MOLECULAR STABILITY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; SITE DIRECTED MUTAGENESIS; AMINO ACID SEQUENCE; ANIMAL; CHEMISTRY; ENZYME ACTIVE SITE; ENZYMOLOGY; GENETICS; HYDROLYSIS; MOLECULAR GENETICS;

CAENORHABDITIS; CAENORHABDITIS ELEGANS; NEMATODA;

ACID ANHYDRIDE HYDROLASES; AMINO ACID SEQUENCE; ANIMALS; BINDING SITES; CAENORHABDITIS ELEGANS; CATALYTIC DOMAIN; HYDROLYSIS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; SUBSTRATE SPECIFICITY;

EID: 0038813696     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M211983200     Document Type: Article

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