Mutational, kinetic, and NMR studies of the roles of conserved glutamate residues and of lysine-39 in the mechanism of the MutT pyrophosphohydrolase

Biochemistry

Volumn 39, Issue 7, 2000, Pages 1655-1674

  Harris, Thomas K ^a   Wu, Gong ^a   Massiah, Michael A ^a   Mildvan, Albert S ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GLUTAMIC ACID DERIVATIVE; INORGANIC PYROPHOSPHATASE; LYSINE; MAGNESIUM; MANGANESE; METAL COMPLEX; NUCLEOSIDE TRIPHOSPHATASE; PHOSPHORUS;

ARTICLE; BINDING AFFINITY; CATALYSIS; ENZYME ACTIVATION; ENZYME ACTIVE SITE; ENZYME KINETICS; ENZYME SUBSTRATE COMPLEX; LIGAND BINDING; MUTATION; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PRIORITY JOURNAL;

ADENOSINE TRIPHOSPHATE; ASPARTIC ACID; BACTERIAL PROTEINS; BINDING, COMPETITIVE; CATALYSIS; CHLORIDES; CONSERVED SEQUENCE; DNA MUTATIONAL ANALYSIS; ESCHERICHIA COLI PROTEINS; GLUTAMIC ACID; GLUTAMINE; KINETICS; LYSINE; MAGNESIUM; MAGNESIUM CHLORIDE; MANGANESE; MANGANESE COMPOUNDS; MUTAGENESIS, SITE-DIRECTED; NITROGEN ISOTOPES; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PHOSPHORIC MONOESTER HYDROLASES; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN STRUCTURE, TERTIARY; PROTONS; PYROPHOSPHATASES; RECOMBINANT PROTEINS; SOLVENTS;

FELIS CATUS;

EID: 0034701056     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi9918745     Document Type: Article

References (46)

1. Bhatnagar, S. K., Bullions, L. C., and Bessman, M. J. (1991) J. Biol. Chem. 266, 9050-9054.
2. Weber, D. J., Abeygunawardana, C., Bessman, M. J., and Mildvan, A. S. (1993) Biochemistry 32, 13081-13088.
3. Mildvan, A. S., Weber, D. J., and Abeygunawardana, C. (1999) Adv. Enzymol. Relut. Amis Mol. Biol. 73, 183-207.
4. Yanofsky, C., Cox, E. C., and Horn, V. (1966) Proc. Natl. Acad. Sci. U.S.A. 55, 274-281.
5. Maki, H., and Sekiguchi, M. (1992) Nature 355, 273-275.
6. Cheng, K. C., Cahill, D. S., Kasai, H., Nishimura, S., and Loeb, L. A. (1992) J. Biol. Chem. 267, 166-172.
7. Mo, J. Y., Maki, H., and Sekiguchi, M. (1992) Proc. Natl. Acad. Sci. U.S.A. 89, 11021-11025.
8. Sakumi, K., Furuichi, M., Tsuzuki, T., Kakuma, T., Kawabata, S., Maki, H., and Sekiguchi, M. (1993) J. Biol. Chem. 268, 23524-23530.
9. Kakuma, T., Nishida, J., Tsuzuki, T., and Sekiguchi, M. (1995) J. Biol. Chem. 270, 25942-25948.
10. Frick, D. N., Weber, D. J., Gillespie, J. R., Bessman, M. J., and Mildvan, A. S. (1994) J. Biol. Chem. 269, 1794-1803.
11. Abeygunawardana, C., Weber, D. J., Gittis, A. G., Frick, D. N., Lin, J., Miller, A.-F., Bessman, M. J., and Mildvan, A. S. (1995) Biochemistry 34, 14997-15005.
12. Lin, J., Abeygunawardana, C., Frick, D. N., Bessman, M. J., and Mildvan, A. S. (1997) Biochemistry 36, 1199-1211.
13. Lin, J., Abeygunawardana, C., Frick, D. N., Bessman, M. J., and Mildvan, A. S. (1996) Biochemistry 35, 6715-6726.
14. Frick, D. N., Weber, D. J., Abeygunawardana, C., Gittis, A. G., Bessman, M. J., and Mildvan, A. S. (1995) Biochemistry 34, 5577-5586.
15. Harris, T. K., Wu, G., and Mildvan, A. S. (1999) FASEB J. 13, A1353.
16. Wu, G., Harris, T. K., and Mildvan, A. S. (1999) FASEB J. 13, A1353.
17. Abeygunawardana, C., Weber, D. J., Frick, D. N., Bessman, M. J., and Mildvan, A. S. (1993) Biochemistry 32, 13071-13080.
18. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) in Molecular Cloning, Cold Spring Harbor Laboratory Press, Plainview, NY.
19. Sarkar, G., and Sommer, S. S. (1990) BioTechniques 8, 404-407.
20. Barik, S. (1993) in PCR Protocol: Current Methods and Applications (White, B. A., Ed.) Vol. 15, pp 277-286, Humana Press, Inc., Totowa, NJ.
21. Barnes, W. M. (1994) Proc. Natl. Acad. Sci. U.S.A. 91, 2216-2220.
22. Cohn, M., and Townsend, J. (1954) Nature 173, 1090-1091.
23. Mildvan, A. S., and Engle, J. L. (1972) Methods Enzymol. 26C, 654-682.
24. Piotto, M., Saudek, V., and Sklenar, V. (1992) J. Biomol. NMR 2, 661-665.
25. Marion, D., Driscoll, P. C., Kay, L. E., Wingfield, P. T., Bax, A., Gronenborn, A. M., and Clore, G. M. (1989) Biochemistry 28, 6150-6156.
26. Kay, L. E., Keifer, P., and Saarinen, T. (1992) J. Am. Chem. Soc. 114, 10663-10665.
27. Levy, G. C., and Lichter, R. L. (1979) in Nitrogen-15 NMR Spectroscopy, John Wiley & Sons, New York.
28. Zhang, O., Kay, L. E., Olivier, J. P., and Foreman-Kay, J. D. (1994) J. Biomol. NMR 4, 845-858.
29. Kay, L. E. (1993) J. Am. Chem. Soc. 115, 2055-2057.
30. Johnson, B. A., and Blevins, R. A. (1994) J. Biomol. NMR 4, 603.
31. Dawson, R. M. C., Elliot, D. C., Elliot, W. H., and Jones, K. M. (1986) in Data for Biochemical Research, pp 413-415, Oxford University Press, New York.
32. Segel, I. H. (1977) in Enzyme Kinetics, pp 884-924, John Wiley & Sons, New York.
33. Mildvan, A. S., and Gupta, R. K. (1978) Methods Enzymol. 49G, 322-359.
34. Serpersu, E. H., Shortle, D., and Mildvan, A. S. (1986) Biochemistry 25, 68-77.
35. Serpersu, E. H., Shortle, D., and Mildvan, A. S. (1987) Biochemistry 26, 1289-1300.
36. Zhao, Q., Abeygunawardana, C., and Mildvan, A. S. (1997) Biochemistry 36, 3458-3472.
37. Villafranca, J. J., and Mildvan, A. S. (1972) J. Biol. Chem. 247, 3454-3463.
38. Mildvan, A. S. (1997) Proteins: Struct., Funct., Genet. 29, 401-416.
39. Cotton, F. A., and Wilkinson, G. (1980) in Advanced Inorganic Chemistry, pp 71-74, John Wiley & Sons, New York.
40. Basolo, F., and Pearson, R. G. (1967) in Mechanisms of Inorganic Reactions, pp 223-228, John Wiley & Sons, New York.
41. Focia, P. J., Craig, S. P., III, and Eakin, A. E. (1998) Biochemistry 37, 17120-17127.
42. Serpersu, E. H., McCracken, J., Peisach, J., and Mildvan, A. S. (1988) Biochemistry 27, 8034-8044.
43. Cameron, A. D., Ridderström, M., Olin, B., Kavarana, M. J., Creighton, D. J., and Mannervik, B. (1999) Biochemistry 38, 13480-13490.
44. Harris, T. K., Cole, R. N., Comer, F. I., and Mildvan, A. S. (1998) Biochemistry 37, 16828-16838.
45. Kuliopulos, A., Mildvan, A. S., Shortle, D., and Talalay, P. (1989) Biochemistry 28, 149-159.
46. Mildvan, A. S., Weber, D. J., and Kuliopulos, A. (1992) Arch. Biochem. Biophys. 294, 327-340.